Transcriptome-wide RNA structure dynamicsDevelopment of NGS-based methods for tracking of RNA structure-dependent regulation of gene expression
In recent years RNA is emerging as the key regulator of gene expression. In most of known cases, the regulatory function fulfilled by RNA molecule is highly dependent on the accommodated secondary and tertiary structure.
The aim of the project is to develop both, the experimental and computational methods for identification of novel RNA-based regulatory mechanisms, relying solely on dynamic changes in RNA structure. Our research focuses on high throughput, transcriptome-wide approaches based on NGS technology.
Integrated genomics of maizeRevealing the molecular mechanisms underlying the differential herbicide resistance of maize lines
Maize is one of the most important crops in world's economy. In order to increase the production, the genetically modified lines are used, which feature increased resistance to herbicides. However similar levels of resistance can be acquired by plants in non-GMO manner, which is of special interest lately, considering public acceptance of GMO.
Within this project we are looking for genetic and molecular mechanism which could lead to gaining the herbicide resistance without introduction of foreign genes into plant system. We are trying to achieve the goal by multi-level analysis of two maize lines obtained with classical breeding methods which reveal substantial differences in glyphosate susceptibility. Our main scope is the computational analysis of the genome, epigenome and transcriptome sequencing data obtained from both lines.