Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.48574 | 0.0324717 | 5.55825 | 0.161088 | |
| 10day-seedling | 3.32864 | 0.251788 | 5.13679 | 0.160538 | |
| 14day-seedling | 3.57783 | 0.439027 | 4.74953 | 0.379516 | |
| 25day-aerial | 3.38076 | 0.254319 | 4.99315 | 0.380124 | |
| 35day-aerial | 3.34392 | 0.206259 | 5.13575 | 0.100163 | |
| 42day-rosette | 3.37047 | 0.221623 | 5.2238 | 0.182885 | 42day-stem | 3.38688 | 0.0495498 | 5.10937 | 0.140245 |
| 53day-rosette | 3.78904 | 0.440802 | 5.63155 | 0.392156 | 53day-stem | 3.71248 | 0.565173 | 5.21086 | 0.14386 | 53day-inflores | 3.7707 | 0.0876331 | 5.205 | 0.023258 | 53day-silique | 4.30914 | 0.113565 | 5.45174 | 0.11561 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 28.8 | 4.95 | 3.24e-2 | -1.49 | 4.86 | 3.44e-2 | -1.46 |
| 2 | 1.000 | 33.13 | 5.15 | 2.81e-2 | -1.55 | 5.29 | 2.56e-2 | -1.59 | |
| 3 | 1.000 | 29.26 | 4.99 | 3.14e-2 | -1.5 | 4.22 | 5.36e-2 | -1.27 | |
| 10day-seedling | 1 | 1.000 | 24.97 | 12.11 | 2.26e-4 | -3.65 | 6.62 | 1.02e-2 | -1.99 |
| 2 | 1.000 | 24.65 | 11.41 | 3.69e-4 | -3.43 | 5.59 | 2.07e-2 | -1.68 | |
| 3 | 1.000 | 24.44 | 13.07 | 1.16e-4 | -3.93 | 6.36 | 1.22e-2 | -1.91 | |
| 14day-seedling | 1 | 0.999 | 26.33 | 12.75 | 1.45e-4 | -3.84 | 6.11 | 1.45e-2 | -1.84 |
| 2 | 0.000 | 29.61 | 11.5 | 3.45e-4 | -3.46 | 8.6 | 2.58e-3 | -2.59 | |
| 3 | 0.998 | 27.64 | 9.85 | 1.09e-3 | -2.96 | 7.71 | 4.78e-3 | -2.32 | |
| 25day-aerial | 1 | 0.999 | 24.81 | 11.95 | 2.53e-4 | -3.6 | 6.19 | 1.37e-2 | -1.86 |
| 2 | 0.995 | 26.95 | 12.9 | 1.31e-4 | -3.88 | 6.66 | 9.90e-3 | -2 | |
| 3 | 0.998 | 25.1 | 11.22 | 4.20e-4 | -3.38 | 5.65 | 1.99e-2 | -1.7 | |
| 35day-aerial | 1 | 1.000 | 25.81 | 11.36 | 3.81e-4 | -3.42 | 5.82 | 1.77e-2 | -1.75 |
| 2 | 1.000 | 25.31 | 12.59 | 1.62e-4 | -3.79 | 6.46 | 1.14e-2 | -1.94 | |
| 3 | 0.000 | 25.68 | 12.49 | 1.74e-4 | -3.76 | 6.3 | 1.27e-2 | -1.9 | |
| 42day-rosette | 1 | 1.000 | 26.17 | 12.63 | 1.57e-4 | -3.8 | 5.53 | 2.17e-2 | -1.66 |
| 2 | 1.000 | 25.64 | 11.26 | 4.07e-4 | -3.39 | 5.57 | 2.10e-2 | -1.68 | |
| 3 | 1.000 | 25.69 | 12.17 | 2.17e-4 | -3.66 | 6.6 | 1.03e-2 | -1.99 | |
| 42day-stem | 1 | 1.000 | 23.51 | 11.82 | 2.77e-1 | -3.56 | 6.59 | 1.99e-1 | -1.96 |
| 2 | 1.000 | 24.68 | 12.14 | 2.22e-1 | -3.65 | 6.59 | 1.39e-1 | -1.98 | |
| 3 | 1.000 | 25.43 | 12.52 | 2.36e-1 | -3.63 | 5.74 | 1.87e-1 | -1.73 | |
| 53day-rosette | 1 | 1.000 | 23.08 | 9.03 | 1.91e-3 | -2.72 | 3.05 | 1.21e-1 | -0.92 |
| 2 | 1.000 | 23.39 | 11.1 | 4.55e-4 | -3.34 | 5.39 | 2.39e-2 | -1.62 | |
| 3 | 1.000 | 24.76 | 11.86 | 2.68e-4 | -3.57 | 5.21 | 2.71e-2 | -1.57 | |
| 53day-stem | 1 | 0.999 | 26.25 | 12.83 | 1.38e-4 | -3.86 | 6.31 | 1.26e-2 | -1.9 |
| 2 | 1.000 | 24.83 | 10.06 | 9.40e-4 | -3.03 | 5.4 | 2.36e-2 | -1.63 | |
| 3 | 0.999 | 26.68 | 9.07 | 1.86e-3 | -2.73 | 6.12 | 1.44e-2 | -1.84 | |
| 53day-inflores | 1 | 0.999 | 24.45 | 11.06 | 4.68e-4 | -3.33 | 5.91 | 1.67e-2 | -1.78 |
| 2 | 0.999 | 25.23 | 10.6 | 6.43e-4 | -3.19 | 6.05 | 1.51e-2 | -1.82 | |
| 3 | 0.999 | 24.35 | 10.52 | 6.81e-4 | -3.17 | 5.93 | 1.64e-2 | -1.79 | |
| 53day-silique | 1 | 1.000 | 28.8 | 8.95 | 2.02e-3 | -2.69 | 5 | 3.13e-2 | -1.51 |
| 2 | 1.000 | 27.85 | 8.56 | 2.66e-3 | -2.58 | 4.85 | 3.46e-2 | -1.46 | |
| 3 | 1.000 | 28.28 | 9.31 | 1.58e-3 | -2.8 | 5.58 | 2.09e-2 | -1.68 | |
NGS expression data
Expression of miR861 transcript in NGS dataSequences
miRNA transcript
GGGGCUUUGGUGAAGGAUUAAGGCUGAUUUAUGGAGUGUAUAAAUUGGUAAAAUCUUCAUUACCAUGUAUCAUUGUUUUCGAACGAUGUCCUUGGAGAAAUAUGCGUCAAUGGAGUCCGUCUUCUUGAGAAGCAUUUUUGAGUGAGAAUGAUUCUCAUGAUGGAUAUGUCUUCAAGGACUUCUUUCAAACAAUGGUAC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
GGGCTTTGGTGAAGGATTAAGG | primer | 5p | 2 | 23 |
GACGGACTCCATTGACGCATAT | primer | 3p | 100 | 121 |
CCUUGGAGAAAUAUGCGUCAA | sRNA | 5p | 90 | 110 |
GAUGGAUAUGUCUUCAAGGAC | sRNA | 3p | 159 | 179 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR861 found in PubMed |