Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.46031 | 0.723911 | 4.53282 | 0.663175 | |
| 10day-seedling | 2.77722 | 0.25152 | 4.58537 | 0.197372 | |
| 14day-seedling | 1.73207 | 0.734021 | 2.90482 | 1.45628 | |
| 25day-aerial | 2.33205 | 0.0482278 | 4.09414 | 0.109237 | |
| 35day-aerial | 2.93847 | 0.154208 | 4.73149 | 0.0603602 | |
| 42day-rosette | 2.56718 | 0.291954 | 4.64036 | 0.364559 | 42day-stem | 2.35529 | 0.341442 | 4.37454 | 0.102617 |
| 53day-rosette | 3.61992 | 0.700854 | 5.46243 | 0.669758 | 53day-stem | 5.55336 | 3.67442 | 6.9709104 | 4.15262 | 53day-inflores | 3.08305 | 0.0427502 | 4.51718 | 0.0849875 | 53day-silique | 3.02419 | 0.918297 | 4.16678 | 0.953293 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.19 | 10.33 | 7.77e-4 | -3.11 | 10.24 | 8.25e-4 | -3.08 |
| 2 | 1.000 | 33.71 | 5.73 | 1.88e-2 | -1.73 | 5.86 | 1.72e-2 | -1.77 | |
| 3 | 1.000 | 33.52 | 9.25 | 1.64e-3 | -2.78 | 8.48 | 2.80e-3 | -2.55 | |
| 10day-seedling | 1 | 1.000 | 25.93 | 13.07 | 1.17e-4 | -3.93 | 7.57 | 5.25e-3 | -2.28 |
| 2 | 1.000 | 27.83 | 14.59 | 4.06e-5 | -4.39 | 8.77 | 2.28e-3 | -2.64 | |
| 3 | 1.000 | 25.8 | 14.43 | 4.53e-5 | -4.34 | 7.72 | 4.75e-3 | -2.32 | |
| 14day-seedling | 1 | 1.000 | 28.38 | 14.81 | 3.48e-5 | -4.46 | 8.16 | 3.49e-3 | -2.46 |
| 2 | 0.000 | 36.24 | 18.13 | 3.49e-6 | -5.46 | 15.22 | 2.62e-5 | -4.58 | |
| 3 | 1.000 | 37.35 | 19.56 | 1.29e-6 | -5.89 | 17.42 | 5.69e-6 | -5.25 | |
| 25day-aerial | 1 | 0.996 | 28.54 | 15.68 | 1.91e-5 | -4.72 | 9.92 | 1.03e-3 | -2.99 |
| 2 | 0.984 | 29.52 | 15.48 | 2.19e-5 | -4.66 | 9.24 | 1.66e-3 | -2.78 | |
| 3 | 1.000 | 29.24 | 15.36 | 2.37e-5 | -4.62 | 9.8 | 1.12e-3 | -2.95 | |
| 35day-aerial | 1 | 0.998 | 27.37 | 12.92 | 1.29e-4 | -3.89 | 7.38 | 6.01e-3 | -2.22 |
| 2 | 1.000 | 26.62 | 13.89 | 6.57e-5 | -4.18 | 7.76 | 4.61e-3 | -2.34 | |
| 3 | 0.000 | 26.85 | 13.65 | 7.76e-5 | -4.11 | 7.47 | 5.65e-3 | -2.25 | |
| 42day-rosette | 1 | 0.999 | 27.08 | 13.54 | 8.38e-5 | -4.08 | 6.44 | 1.15e-2 | -1.94 |
| 2 | 0.998 | 28.63 | 14.24 | 5.16e-5 | -4.29 | 8.55 | 2.66e-3 | -2.57 | |
| 3 | 1.000 | 27.61 | 14.09 | 5.73e-5 | -4.24 | 8.52 | 2.72e-3 | -2.57 | |
| 42day-stem | 1 | 0.999 | 25.31 | 13.62 | 7.94e-1 | -5 | 8.4 | 3.15e-1 | -2.51 |
| 2 | 0.997 | 26.86 | 14.32 | 4.89e-1 | -4.32 | 8.77 | 2.29e-1 | -2.64 | |
| 3 | 1.000 | 28.72 | 15.33 | 2.42e-1 | -4.62 | 9.26 | 1.92e-1 | -2.72 | |
| 53day-rosette | 1 | 1.000 | 22.6 | 8.55 | 2.67e-3 | -2.57 | 2.56 | 1.70e-1 | -0.77 |
| 2 | 1.000 | 24.67 | 12.38 | 1.87e-4 | -3.73 | 6.67 | 9.85e-3 | -2.01 | |
| 3 | 1.000 | 25.65 | 12.75 | 1.45e-4 | -3.84 | 6.1 | 1.46e-2 | -1.84 | |
| 53day-stem | 1 | 0.837 | 4.13 | -9.29 | 6.25e+2 | 2.8 | -15.81 | 5.73e+4 | 4.76 |
| 2 | 1.000 | 26.71 | 11.93 | 2.55e-4 | -3.59 | 7.28 | 6.43e-3 | -2.19 | |
| 3 | 1.000 | 29.37 | 11.77 | 2.86e-4 | -3.54 | 8.82 | 2.22e-3 | -2.65 | |
| 53day-inflores | 1 | 0.993 | 26.49 | 13.09 | 1.14e-4 | -3.94 | 7.94 | 4.07e-3 | -2.39 |
| 2 | 1.000 | 27.48 | 12.85 | 1.36e-4 | -3.87 | 8.29 | 3.19e-3 | -2.5 | |
| 3 | 1.000 | 26.92 | 13.09 | 1.14e-4 | -3.94 | 8.5 | 2.76e-3 | -2.56 | |
| 53day-silique | 1 | 1.000 | 30.24 | 10.39 | 7.45e-4 | -3.13 | 6.44 | 1.15e-2 | -1.94 |
| 2 | 1.000 | 35.74 | 16.45 | 1.12e-5 | -4.95 | 12.74 | 1.46e-4 | -3.84 | |
| 3 | 1.000 | 31.75 | 12.78 | 1.42e-4 | -3.85 | 9.05 | 1.88e-3 | -2.72 | |
NGS expression data
Expression of miR853 transcript in NGS dataSequences
miRNA transcript
GAGAGGAACAAUUAGGGUUGUUUCAAACAGGGUAAAGUGUUCCUGCUGCUCCCCUCUUUAGCUUGGAGAAGCCAGUAAAUAUCUUUCUCUUCAAGGAACUUGUGGGCCUCAAGAAAACCUGCUAUAAUUCUAGUUUUUCAACAUUCCUCCUUCUGAUAUCAGCAACAACUCCUUACCCUUAAGAAUGGAGAAUGGAACUUCCUCAAAGAUAUUUACUUGGCCUCUGCAACCGGAAAGGGGAGCAGCAUGAAACUCUUUUAACCCUUACCCUGUAUUUGUCAAAAACAGAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCCCCUCUUUAGCUUGGAGAAG | miRNA | 50 | 71 | |
TTCCTGCTGCTCCCCTCTTTA | primer | 5p | 40 | 60 |
CGGTTGCAGAGGCCAAGTAAAT | primer | 3p | 211 | 232 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR853 found in PubMed |