Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 1.43852 | 0 | 1.67015 | 0 | |
| 10day-seedling | 2.01784 | 0.285884 | 3.82599 | 0.173497 | |
| 14day-seedling | 1.34453 | 0 | 3.34468 | 0 | |
| 25day-aerial | 0.09382 | 1.11607 | 1.8006 | 1.0706 | |
| 35day-aerial | 0.67965 | 0.219921 | 2.45014 | 0.214086 | |
| 42day-rosette | 0.69135 | 0.714502 | 2.5431 | 0.85823 | 42day-stem | 1.09525 | 0.526623 | 2.87514 | 0.369605 |
| 53day-rosette | 1.17109 | 0.584462 | 3.0136 | 0.478631 | 53day-stem | 1.99354 | 1.22907 | 3.16143 | 0.490632 | 53day-inflores | 1.47315 | 0.935844 | 2.62389 | 0.4355 | 53day-silique | 3.11165 | 0 | 4.30086 | 0 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 42.75 | 18.47 | 2.74e-6 | -5.56 | 17.71 | 4.68e-6 | -5.33 | |
| 10day-seedling | 1 | 1.000 | 29.25 | 16.39 | 1.17e-5 | -4.93 | 10.9 | 5.25e-4 | -3.28 |
| 2 | 1.000 | 28.94 | 15.69 | 1.89e-5 | -4.72 | 9.88 | 1.06e-3 | -2.97 | |
| 3 | 1.000 | 28.94 | 17.57 | 5.14e-6 | -5.29 | 10.86 | 5.39e-4 | -3.27 | |
| 14day-seedling | 1 | 1.000 | 32.36 | 18.79 | 2.21e-6 | -5.66 | 12.14 | 2.21e-4 | -3.66 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | 9.999 | 38.4 | 25.55 | 2.04e-8 | -7.69 | 19.79 | 1.11e-6 | -5.96 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.999 | 34.2 | 20.32 | 7.64e-7 | -6.12 | 14.76 | 3.61e-5 | -4.44 | |
| 35day-aerial | 1 | 0.999 | 34.99 | 20.53 | 6.59e-7 | -6.18 | 15 | 3.06e-5 | -4.51 |
| 2 | 1.000 | 33.31 | 20.59 | 6.36e-7 | -6.2 | 14.45 | 4.46e-5 | -4.35 | |
| 3 | 0.999 | 35.02 | 21.83 | 2.69e-7 | -6.57 | 15.64 | 1.96e-5 | -4.71 | |
| 42day-rosette | 1 | 0.999 | 33.15 | 19.61 | 1.25e-6 | -5.9 | 12.5 | 1.72e-4 | -3.76 |
| 2 | 0.996 | 38.07 | 23.69 | 7.41e-8 | -7.13 | 17.99 | 3.83e-6 | -5.42 | |
| 3 | 1.000 | 33.01 | 19.49 | 1.36e-6 | -5.87 | 13.92 | 6.46e-5 | -4.19 | |
| 42day-stem | 1 | 0.999 | 29.29 | 17.6 | 5.49e-1 | -5.3 | 12.29 | 2.32e-1 | -3.7 |
| 2 | 1.000 | 32.54 | 20 | 9.55e-1 | -6.2 | 14.45 | 4.47e-1 | -4.35 | |
| 3 | 0.998 | 34.04 | 2.66 | 6.37e-7 | -6.22 | 14.35 | 4.78e-1 | -4.33 | |
| 53day-rosette | 1 | 1.000 | 31.65 | 17.6 | 5.04e-6 | -5.3 | 11.61 | 3.20e-4 | -3.5 |
| 2 | 1.000 | 31.33 | 19.05 | 1.85e-6 | -5.73 | 13.33 | 9.70e-5 | -4.01 | |
| 3 | 1.000 | 34.33 | 21.44 | 3.51e-7 | -6.46 | 14.79 | 3.54e-5 | -4.45 | |
| 53day-stem | 1 | 0.999 | 34.44 | 21.02 | 4.70e-7 | -6.33 | 14.5 | 4.31e-5 | -4.37 |
| 2 | 1.000 | 30.7 | 15.93 | 1.60e-5 | -4.79 | 11.28 | 4.03e-4 | -3.39 | |
| 3 | 1.000 | 30.55 | 12.94 | 1.27e-4 | -3.9 | 9.99 | 9.83e-4 | -3.01 | |
| 53day-inflores | 1 | 0.999 | 34.17 | 20.78 | 5.55e-7 | -6.26 | 15.63 | 1.98e-5 | -4.7 |
| 2 | 1.000 | 34.07 | 19.44 | 1.40e-6 | -5.85 | 14.89 | 3.30e-5 | -4.48 | |
| 3 | 0.994 | 28.68 | 14.85 | 3.38e-5 | -4.47 | 10.26 | 8.13e-4 | -3.09 | |
| 53day-silique | 1 | 1.000 | 32.76 | 12.92 | 1.29e-4 | -3.89 | 8.97 | 2.00e-3 | -2.7 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
NGS expression data
Expression of miR837 transcript in NGS dataSequences
miRNA transcript
AAGUCAUGCCCUUCAUCAUUGGUAUUUAUUCAUCCAUAUUCUUUCUUAUAUAUAUAUAUAUAUAUUUUUUUUUUUUGAUAAAUACUUUUGUUUGCUUACAAGUAUAAAGCCAUCAUGUUUUUUUCAUCAGUUUCUUGUUCGUUUCAAAAUUAUGAUUUUUGUUUACUUACACUCAUAAUCUUGAAACGAACAAAAAACUGAUGGAAAAACAUGAGGACUUUAUACUUGUAAAUAAAUAAAAAUAUUUAUUAGAAA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
AGTCATGCCCTTCATCATTGGTA | primer | 5p | 2 | 24 |
TGAAACGAACAAGAAACTGATGAAAA | primer | 3p | 121 | 146 |
AUCAGUUUCUUGUUCGUUUCA | sRNA | 5p | 126 | 146 |
AAACGAACAAAAAACUGAUGG | sRNA | 3p | 184 | 204 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR837 found in PubMed |