Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 2.78661 | 0.27809 | 2.85912 | 0.394487 | |
| 10day-seedling | 0.45817 | 0.331647 | 2.26632 | 0.181621 | |
| 14day-seedling | 0.70252 | 0 | 2.70274 | 0 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | -0.30624 | 0.476263 | 1.43238 | 0.332324 | |
| 42day-rosette | -0.36259 | 0.727287 | 1.72666 | 0.195701 | 42day-stem | -0.05701 | 0.430415 | 1.73859 | 0.319139 |
| 53day-rosette | 0.30107 | 0.501687 | 2.14357 | 0.367631 | 53day-stem | 0.14943 | 1.4437 | 1.97181 | 0.136515 | 53day-inflores | 1.02355 | 0.379025 | 2.45849 | 0.480022 | 53day-silique | 2.26252 | 0.302963 | 3.40511 | 0.281267 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 37.39 | 13.53 | 8.46e-5 | -4.07 | 13.44 | 8.98e-5 | -4.05 |
| 2 | 1.000 | 43.04 | 15.06 | 2.93e-5 | -4.53 | 15.19 | 2.67e-5 | -4.57 | |
| 3 | 1.000 | 37.67 | 13.4 | 9.25e-5 | -4.03 | 12.63 | 1.58e-4 | -3.8 | |
| 10day-seedling | 1 | 1.000 | 33.39 | 20.53 | 6.61e-7 | -6.18 | 15.04 | 2.98e-5 | -4.53 |
| 2 | 1.000 | 35.22 | 21.97 | 2.43e-7 | -6.61 | 16.16 | 1.37e-5 | -4.86 | |
| 3 | 1.000 | 34.06 | 22.69 | 1.47e-7 | -6.83 | 15.98 | 1.55e-5 | -4.81 | |
| 14day-seedling | 1 | 0.999 | 34.49 | 20.92 | 5.04e-7 | -6.3 | 14.28 | 5.04e-5 | -4.3 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | 0.999 | 36.88 | 22.43 | 1.78e-7 | -6.75 | 16.89 | 8.24e-6 | -5.08 |
| 2 | 0.994 | 37.66 | 24.94 | 3.11e-8 | -7.51 | 18.8 | 2.18e-6 | -5.66 | |
| 3 | 0.998 | 36.64 | 23.45 | 8.72e-8 | -7.06 | 17.26 | 6.36e-6 | -5.2 | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.998 | 37.13 | 22.75 | 1.42e-7 | -6.85 | 17.06 | 7.33e-6 | -5.13 | |
| 3 | 1.000 | 37.07 | 23.54 | 8.17e-8 | -7.09 | 17.98 | 3.88e-6 | -5.41 | |
| 42day-stem | 1 | 0.998 | 35.03 | 23.33 | 9.46e-8 | -7.24 | 18.24 | 3.75e-6 | -5.43 |
| 2 | 0.998 | 34.35 | 21.85 | 2.73e-1 | -6.56 | 16.26 | 1.28e-1 | -4.89 | |
| 3 | 1.000 | 37.84 | 24.47 | 4.33e-8 | -7.36 | 18.15 | 3.43e-6 | -5.46 | |
| 53day-rosette | 1 | 1.000 | 36.5 | 22.45 | 1.74e-7 | -6.76 | 16.46 | 1.11e-5 | -4.96 |
| 2 | 1.000 | 32.78 | 20.5 | 6.76e-7 | -6.17 | 14.78 | 3.55e-5 | -4.45 | |
| 3 | 1.000 | 36.7 | 23.81 | 6.79e-8 | -7.17 | 17.15 | 6.86e-6 | -5.16 | |
| 53day-stem | 1 | 0.998 | 36.97 | 23.54 | 8.18e-8 | -7.09 | 17.02 | 7.50e-6 | -5.12 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.000 | 36.94 | 19.34 | 1.51e-6 | -5.82 | 16.38 | 1.17e-5 | -4.93 | |
| 53day-inflores | 1 | 1.000 | 31.79 | 18.4 | 2.89e-6 | -5.54 | 13.25 | 1.03e-4 | -3.99 |
| 2 | 0.999 | 35.21 | 20.59 | 6.36e-7 | -6.2 | 16.03 | 1.49e-5 | -4.83 | |
| 3 | 0.996 | 34.4 | 20.57 | 6.40e-7 | -6.19 | 15.98 | 1.54e-5 | -4.81 | |
| 53day-silique | 1 | 1.000 | 36.16 | 16.31 | 1.23e-5 | -4.91 | 12.36 | 1.90e-4 | -3.72 |
| 2 | 1.000 | 33.87 | 14.58 | 4.10e-5 | -4.39 | 10.87 | 5.34e-4 | -3.27 | |
| 3 | 1.000 | 35.29 | 16.32 | 1.22e-5 | -4.91 | 12.59 | 1.62e-4 | -3.79 | |
NGS expression data
Expression of miR831 transcript in NGS dataSequences
miRNA transcript
GCACAAUGCGGACAGUGCUACAAGAAGAGAUUUAUAGAGUCUUAAGAGUCUCAAGAAGCGUACAAGGAGAUGAGGAGUGAAUCACCAAAACAAGUGGUUUUGGUUUGUGAAUCACCAAAACAAGUGGUUUUGGUUUGUGAAUCAGUAUGGUUUACCCAAAACACUUGUUUGGUGUGUUUACUUCUGAUCUCUUCGUACUCUUCUUGCGAUUCUAUGACACUCUACAUGUAACAUCGGCAUGGUUUACCAAAAAGCCU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGAUCUCUUCGUACUCUUCUUG | miRNA | 185 | 206 | |
CAATGCGGACAGTGCTACAAGA | primer | 5p | 4 | 25 |
CGAAGAGATCAGAAGTAAACACACCAA | primer | 3p | 169 | 195 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR831 found in PubMed |