Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.56171 | 0.580021 | 4.63422 | 0.584353 | |
| 10day-seedling | 1.01257 | 0.647492 | 2.82073 | 0.493196 | |
| 14day-seedling | 2.28769 | 0.220758 | 4.11548 | 0.463764 | |
| 25day-aerial | 1.20502 | 0 | 3.08435 | 0 | |
| 35day-aerial | 0.35772 | 0 | 2.20337 | 0 | |
| 42day-rosette | 1.24138 | 0.169173 | 2.92991 | 0.215751 | 42day-stem | 0.24728 | 1.17161 | 1.87904 | 1.12456 |
| 53day-rosette | 0.38331 | 1.26683 | 2.22582 | 1.12264 | 53day-stem | 1.09605 | 0.39217 | 2.5135 | 0.635424 | 53day-inflores | 1.31785 | 0.727964 | 2.69483 | 0.720112 | 53day-silique | 3.2265 | 1.02466 | 4.36909 | 1.06321 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.02 | 10.17 | 8.70e-4 | -3.06 | 10.08 | 9.24e-4 | -3.03 |
| 2 | 1.000 | 34.33 | 6.35 | 1.22e-2 | -1.91 | 6.49 | 1.11e-2 | -1.95 | |
| 3 | 1.000 | 32.05 | 7.78 | 4.55e-3 | -2.34 | 7.01 | 7.76e-3 | -2.11 | |
| 10day-seedling | 1 | 1.000 | 32.15 | 19.29 | 1.56e-6 | -5.81 | 13.79 | 7.05e-5 | -4.15 |
| 2 | 1.000 | 31.35 | 18.11 | 3.55e-6 | -5.45 | 12.29 | 1.99e-4 | -3.7 | |
| 3 | 1.000 | 33.65 | 22.28 | 1.97e-7 | -6.71 | 15.57 | 2.06e-5 | -4.69 | |
| 14day-seedling | 1 | 1.000 | 28.71 | 15.14 | 2.78e-5 | -4.56 | 8.49 | 2.78e-3 | -2.56 |
| 2 | 1.000 | 31.69 | 13.58 | 8.19e-5 | -4.09 | 10.67 | 6.13e-4 | -3.21 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 33.29 | 19.25 | 1.60e-6 | -5.79 | 13.01 | 1.21e-4 | -3.92 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.999 | 34.79 | 22.07 | 2.28e-7 | -6.64 | 15.93 | 1.60e-5 | -4.8 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.994 | 33.09 | 18.71 | 2.34e-6 | -5.63 | 13.01 | 1.21e-4 | -3.92 | |
| 3 | 1.000 | 33.05 | 19.53 | 1.32e-6 | -5.88 | 13.96 | 6.28e-5 | -4.2 | |
| 42day-stem | 1 | 1.000 | 31.37 | 19.68 | 1.20e-1 | -5.92 | 14.37 | 4.72e-1 | -4.33 |
| 2 | 0.997 | 37.73 | 25.18 | 2.62e-1 | -7.58 | 19.63 | 1.23e-1 | -5.92 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | 1.000 | 35.54 | 21.49 | 3.39e-7 | -6.47 | 15.5 | 2.15e-5 | -4.67 |
| 2 | 1.000 | 30.32 | 18.04 | 3.72e-6 | -5.43 | 12.32 | 1.95e-4 | -3.71 | |
| 3 | 1.000 | 39.3 | 26.41 | 1.12e-8 | -7.95 | 19.75 | 1.13e-6 | -5.95 | |
| 53day-stem | 1 | 0.999 | 32.44 | 19.02 | 1.88e-6 | -5.73 | 12.5 | 1.73e-4 | -3.76 |
| 2 | 1.000 | 35.88 | 21.11 | 4.43e-7 | -6.35 | 16.45 | 1.11e-5 | -4.95 | |
| 3 | 0.998 | 36.32 | 18.71 | 2.33e-6 | -5.63 | 15.76 | 1.80e-5 | -4.74 | |
| 53day-inflores | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.999 | 31.79 | 17.17 | 6.80e-6 | -5.17 | 12.61 | 1.60e-4 | -3.8 | |
| 3 | 0.995 | 34.41 | 20.58 | 6.37e-7 | -6.2 | 15.99 | 1.53e-5 | -4.81 | |
| 53day-silique | 1 | 1.000 | 28.8 | 8.96 | 2.01e-3 | -2.7 | 5.01 | 3.11e-2 | -1.51 |
| 2 | 1.000 | 35.02 | 15.73 | 1.84e-5 | -4.74 | 12.03 | 2.39e-4 | -3.62 | |
| 3 | 1.000 | 31.89 | 12.92 | 1.29e-4 | -3.89 | 9.18 | 1.72e-3 | -2.76 | |
NGS expression data
Expression of miR395f transcript in NGS dataSequences
miRNA transcript
CAGUUUGGGUUGUCGCUAAGGUCAGUUGAGUAAGAGAAGAAGCAUUUGCAAAAGAGACAUAACAUGUAAAGUCAAAUGUCCCCUUGAGUUCCCUUAAACGCUUCAUUGUUCAUACUUUGUUAUCAUCUAUCGAUCGAUCAAUCAAUCUGAUGAACACUGAAGUGUUUGGGGGGACUCUAGGUGACAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CUGAAGUGUUUGGGGGGACUC | miRNA | 157 | 177 | |
CAGTTTGGGTTGTCGCTAAGGT | primer | 5p | 1 | 22 |
ACAAAGTATGAACAATGAAGCGTTTAAG | primer | 3p | 93 | 120 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR395f found in PubMed |