Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.65348 | 0.470917 | 3.72599 | 0.3936 | |
| 10day-seedling | 1.53203 | 0.509659 | 3.34018 | 0.369503 | |
| 14day-seedling | 2.22372 | 0 | 4.22343 | 0 | |
| 25day-aerial | 1.05039 | 0.0189068 | 2.57103 | 0.198967 | |
| 35day-aerial | 0.77498 | 0.801586 | 2.82643 | 0.609423 | |
| 42day-rosette | 0.73669 | 0.911622 | 2.75836 | 0.670686 | 42day-stem | 1.43001 | 0.186067 | 3.1466 | 0.314703 |
| 53day-rosette | 1.22124 | 0.756691 | 3.06375 | 0.616513 | 53day-stem | 2.78933 | 1.25493 | 4.23287 | 0.856902 | 53day-inflores | 1.6922 | 0.531918 | 3.23054 | 0.482641 | 53day-silique | 3.71658 | 0.540127 | 4.85917 | 0.579549 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 33.33 | 9.47 | 1.41e-3 | -2.85 | 9.38 | 1.50e-3 | -2.82 |
| 2 | 1.000 | 39.28 | 11.3 | 3.97e-4 | -3.4 | 11.43 | 3.62e-4 | -3.44 | |
| 3 | 1.000 | 36.85 | 12.58 | 1.63e-4 | -3.79 | 11.81 | 2.78e-4 | -3.56 | |
| 10day-seedling | 1 | 1.000 | 29.1 | 16.24 | 1.29e-5 | -4.89 | 10.75 | 5.81e-4 | -3.24 |
| 2 | 1.000 | 32.06 | 18.81 | 2.17e-6 | -5.66 | 13 | 1.22e-4 | -3.91 | |
| 3 | 1.000 | 30.81 | 19.44 | 1.41e-6 | -5.85 | 12.73 | 1.48e-4 | -3.83 | |
| 14day-seedling | 1 | 0.999 | 29.44 | 15.87 | 1.67e-5 | -4.78 | 9.22 | 1.67e-3 | -2.78 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.999 | 33.76 | 19.72 | 1.16e-6 | -5.94 | 13.48 | 8.77e-5 | -4.06 | |
| 3 | 0.999 | 33.69 | 19.81 | 1.09e-6 | -5.96 | 14.25 | 5.15e-5 | -4.29 | |
| 35day-aerial | 1 | 1.000 | 34.67 | 20.22 | 8.20e-7 | -6.09 | 14.68 | 3.81e-5 | -4.42 |
| 2 | 0.996 | 30.41 | 17.69 | 4.72e-6 | -5.33 | 11.56 | 3.31e-4 | -3.48 | |
| 3 | 0.999 | 34.73 | 21.54 | 3.28e-7 | -6.48 | 15.35 | 2.39e-5 | -4.62 | |
| 42day-rosette | 1 | 1.000 | 36.79 | 23.25 | 1.00e-7 | -7 | 16.14 | 1.38e-5 | -4.86 |
| 2 | 0.999 | 34.48 | 20.1 | 8.90e-7 | -6.05 | 14.41 | 4.60e-5 | -4.34 | |
| 3 | 1.000 | 30.81 | 17.29 | 6.25e-6 | -5.2 | 11.72 | 2.96e-4 | -3.53 | |
| 42day-stem | 1 | 0.997 | 30.65 | 18.96 | 1.96e-1 | -5.78 | 13.65 | 7.77e-1 | -4.2 |
| 2 | 1.000 | 30.65 | 18.19 | 3.54e-1 | -5.45 | 12.56 | 1.66e-1 | -3.79 | |
| 3 | 1.000 | 31.57 | 18.18 | 3.36e-6 | -5.47 | 11.88 | 2.66e-1 | -3.58 | |
| 53day-rosette | 1 | 1.000 | 31.89 | 17.84 | 4.27e-6 | -5.37 | 11.85 | 2.71e-4 | -3.57 |
| 2 | 1.000 | 29.94 | 17.65 | 4.85e-6 | -5.31 | 11.94 | 2.55e-4 | -3.59 | |
| 3 | 1.000 | 34.99 | 22.1 | 2.23e-7 | -6.65 | 15.44 | 2.25e-5 | -4.65 | |
| 53day-stem | 1 | 1.000 | 29.4 | 15.97 | 1.55e-5 | -4.81 | 9.45 | 1.42e-3 | -2.85 |
| 2 | 1.000 | 31.33 | 16.55 | 1.04e-5 | -4.98 | 11.9 | 2.62e-4 | -3.58 | |
| 3 | 0.998 | 26.78 | 9.18 | 1.73e-3 | -2.76 | 6.22 | 1.34e-2 | -1.87 | |
| 53day-inflores | 1 | 0.995 | 32.53 | 19.14 | 1.73e-6 | -5.76 | 13.98 | 6.18e-5 | -4.21 |
| 2 | 0.999 | 31.38 | 16.75 | 9.06e-6 | -5.04 | 12.2 | 2.13e-4 | -3.67 | |
| 3 | 0.999 | 29.53 | 15.7 | 1.88e-5 | -4.73 | 11.11 | 4.54e-4 | -3.34 | |
| 53day-silique | 1 | 1.000 | 28.7 | 8.86 | 2.16e-3 | -2.67 | 4.91 | 3.33e-2 | -1.48 |
| 2 | 1.000 | 30.96 | 11.67 | 3.07e-4 | -3.51 | 7.97 | 4.00e-3 | -2.4 | |
| 3 | 1.000 | 31.17 | 12.19 | 2.13e-4 | -3.67 | 8.46 | 2.83e-3 | -2.55 | |
NGS expression data
Expression of miR395e transcript in NGS dataSequences
miRNA transcript
AUAUAUAAAACGCCAGUCCCUCCAUUCUUUUUCAAACCCUAACACUCUUAUAAACCGAUUCGCCAAAAUGUAUCUACAAUAUAUUGAUAAUGUAAUAUCUAUAUAUUCAAACAAUCGUCGUGUUGGUCGGAUGUUUUCUAGAGUUCCUCUGAGCACUUCAUUGGAGAUACAAUUUUUUAUAAAAUAGUUUUCUACUGAAGUGUUUGGGGGAACUCCCGGGCUGAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CUGAAGUGUUUGGGGGAACUC | miRNA | 195 | 215 | |
ATATATAAAACGCCAGTCCCTCCATT | primer | 5p | 1 | 26 |
CATCCGACCAACACGACGAT | primer | 3p | 114 | 133 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR395e found in PubMed |