Expression
Switch to: PP2A
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-9).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 6.73929 | 0.966515 | 6.81181 | 0.874424 | |
| 10day-seedling | 3.39893 | 1.11242 | 5.20709 | 1.07607 | |
| 14day-seedling | 3.54536 | 0.435987 | 4.51988 | 1.38911 | |
| 25day-aerial | 3.50915 | 0.609513 | 5.31893 | 0.528753 | |
| 35day-aerial | 3.47107 | 0 | 5.33354 | 0 | |
| 42day-rosette | 3.85089 | 0.697587 | 5.74224 | 0.392991 | 42day-stem | 3.51293 | 0.380964 | 5.26147 | 0.593138 |
| 53day-rosette | 3.47325 | 0.423732 | 5.23503 | 0.481843 | 53day-stem | 3.75989 | 1.42843 | 5.20358 | 1.26523 | 53day-inflores | 3.64736 | 0.0235892 | 5.0066 | 0.456415 | 53day-silique | 5.03175 | 0.659481 | 6.17435 | 0.699873 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 33.21 | 9.35 | 1.53e-3 | -2.81 | 9.26 | 1.63e-3 | -2.79 |
| 2 | 1.000 | 31.78 | 3.8 | 7.17e-2 | -1.14 | 3.94 | 6.53e-2 | -1.18 | |
| 3 | 1.000 | 33.65 | 9.38 | 1.50e-3 | -2.82 | 8.61 | 2.56e-3 | -2.59 | |
| 10day-seedling | 1 | 1.000 | 27.73 | 14.87 | 3.35e-5 | -4.47 | 9.37 | 1.51e-3 | -2.82 |
| 2 | 1.000 | 35.5 | 22.25 | 2.00e-7 | -6.7 | 16.44 | 1.12e-5 | -4.95 | |
| 3 | 1.000 | 30.07 | 18.7 | 2.35e-6 | -5.63 | 11.99 | 2.46e-4 | -3.61 | |
| 14day-seedling | 1 | 1.000 | 30.02 | 16.45 | 1.12e-5 | -4.95 | 9.8 | 1.12e-3 | -2.95 |
| 2 | 0.997 | 37.04 | 18.93 | 2.00e-6 | -5.7 | 16.03 | 1.50e-5 | -4.82 | |
| 3 | 0.994 | 36.76 | 18.97 | 1.95e-6 | -5.71 | 16.83 | 8.58e-6 | -5.07 | |
| 25day-aerial | 1 | 0.998 | 32.84 | 19.98 | 9.64e-7 | -6.02 | 14.22 | 5.22e-5 | -4.28 |
| 2 | 0.995 | 31.83 | 17.78 | 4.44e-6 | -5.35 | 11.54 | 3.36e-4 | -3.47 | |
| 3 | 1.000 | 30.36 | 16.48 | 1.09e-5 | -4.96 | 10.92 | 5.16e-4 | -3.29 | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.997 | 31.56 | 18.37 | 2.96e-6 | -5.53 | 12.18 | 2.16e-4 | -3.67 | |
| 42day-rosette | 1 | 0.997 | 32.28 | 18.74 | 2.28e-6 | -5.64 | 11.64 | 3.14e-4 | -3.5 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 28.99 | 15.47 | 2.21e-5 | -4.66 | 9.9 | 1.05e-3 | -2.98 | |
| 42day-stem | 1 | 0.999 | 30.81 | 19.12 | 1.75e-6 | -5.76 | 13.81 | 6.95e-1 | -4.16 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.999 | 30.72 | 17.33 | 6.59e-6 | -5.22 | 11.26 | 4.80e-1 | -3.32 | |
| 53day-rosette | 1 | 1.000 | 31.41 | 17.36 | 5.93e-6 | -5.23 | 11.38 | 3.76e-4 | -3.42 |
| 2 | 1.000 | 31.64 | 19.35 | 1.49e-6 | -5.83 | 13.64 | 7.84e-5 | -4.11 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | 0.997 | 30.73 | 17.3 | 6.18e-6 | -5.21 | 10.78 | 5.67e-4 | -3.25 |
| 2 | 0.997 | 36.85 | 22.07 | 2.27e-7 | -6.64 | 17.42 | 5.71e-6 | -5.24 | |
| 3 | 0.998 | 30.19 | 12.59 | 1.63e-4 | -3.79 | 9.63 | 1.26e-3 | -2.9 | |
| 53day-inflores | 1 | 1.000 | 31.17 | 17.78 | 4.45e-6 | -5.35 | 12.62 | 1.58e-4 | -3.8 |
| 2 | 0.999 | 32.49 | 17.86 | 4.20e-6 | -5.38 | 13.3 | 9.88e-5 | -4.01 | |
| 3 | 0.998 | 30.55 | 16.72 | 9.27e-6 | -5.03 | 12.13 | 2.23e-4 | -3.65 | |
| 53day-silique | 1 | 1.000 | 30.5 | 10.65 | 6.21e-4 | -3.21 | 6.7 | 9.60e-3 | -2.02 |
| 2 | 1.000 | 33.74 | 14.45 | 4.47e-5 | -4.35 | 10.75 | 5.83e-4 | -3.23 | |
| 3 | 1.000 | 33.42 | 14.44 | 4.48e-5 | -4.35 | 10.71 | 5.96e-4 | -3.22 | |
NGS expression data
Expression of miR395d transcript in NGS dataSequences
miRNA transcript
UUAUGUUCAUGGUGGUAACUAGCAACAACACAAUAAUAAUUCGACUAGAUGUCCUCUAGAGUUCUCCUGAACACUUCAUUGGAAAUUUGUUAUUCAGUAAGCUAACAGUUAAUUCCACUGAAGUGUUUGGGGGAACUCCCGAUGUCAUUUGGUGA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CUGAAGUGUUUGGGGGAACUC | miRNA | 118 | 138 | |
TTATGTTCATGGTGGTAACTAGCAA | primer | 5p | 1 | 25 |
TCACCAAATGACATCGGGAGTTCC | primer | 3p | 132 | 155 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR395d found in PubMed |