Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 2.98022 | 0.519534 | 3.0759 | 0.327276 | |
| 10day-seedling | 3.40169 | 0.681922 | 5.20985 | 0.566387 | |
| 14day-seedling | 3.29538 | 0.252204 | 4.46433 | 0.486749 | |
| 25day-aerial | 3.06371 | 0.62832 | 4.83345 | 0.661049 | |
| 35day-aerial | 3.74572 | 0.0845172 | 5.53755 | 0.0283154 | |
| 42day-rosette | 4.17227 | 0.647965 | 5.97516 | 0.82082 | 42day-stem | 4.96042 | 0.156725 | 6.671781 | 0.0627839 |
| 53day-rosette | 2.62556 | 0.68061 | 4.46806 | 0.575296 | 53day-stem | 5.23728 | 0.16737 | 6.73935 | 0.505145 | 53day-inflores | 4.73831 | 0.141205 | 6.143853 | 0.135762 | 53day-silique | 3.71342 | 0.358832 | 4.85602 | 0.39938 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 40.11 | 12.13 | 2.23e-4 | -3.65 | 12.27 | 2.03e-4 | -3.69 | |
| 3 | 1.000 | 38.85 | 14.57 | 4.10e-5 | -4.39 | 13.8 | 6.99e-5 | -4.16 | |
| 10day-seedling | 1 | 1.000 | 22.2 | 9.34 | 1.55e-3 | -2.81 | 3.84 | 6.97e-2 | -1.16 |
| 2 | 1.000 | 26.53 | 13.28 | 1.00e-4 | -4 | 7.47 | 5.64e-3 | -2.25 | |
| 3 | 1.000 | 24.61 | 13.24 | 1.03e-4 | -3.99 | 6.53 | 1.09e-2 | -1.96 | |
| 14day-seedling | 1 | 1.000 | 26.77 | 13.2 | 1.06e-4 | -3.97 | 6.56 | 1.06e-2 | -1.97 |
| 2 | 0.991 | 30.28 | 12.17 | 2.16e-4 | -3.66 | 9.27 | 1.62e-3 | -2.79 | |
| 3 | 1.000 | 29.33 | 11.54 | 3.35e-4 | -3.47 | 9.4 | 1.48e-3 | -2.83 | |
| 25day-aerial | 1 | 1.000 | 28.26 | 15.4 | 2.31e-5 | -4.64 | 9.64 | 1.25e-3 | -2.9 |
| 2 | 0.999 | 26.35 | 12.31 | 1.97e-4 | -3.71 | 6.07 | 1.49e-2 | -1.83 | |
| 3 | 0.998 | 25.24 | 11.36 | 3.81e-4 | -3.42 | 5.8 | 1.80e-2 | -1.74 | |
| 35day-aerial | 1 | 0.999 | 24.95 | 10.49 | 6.93e-4 | -3.16 | 4.96 | 3.22e-2 | -1.49 |
| 2 | 0.996 | 23.63 | 10.9 | 5.22e-4 | -3.28 | 4.77 | 3.66e-2 | -1.44 | |
| 3 | 0.999 | 24.23 | 11.03 | 4.77e-4 | -3.32 | 4.84 | 3.48e-2 | -1.46 | |
| 42day-rosette | 1 | - | - | 7.11 | 7.24e-3 | -2.14 | - | - | N/D |
| 2 | 1.000 | 25.77 | 11.38 | 3.74e-4 | -3.43 | 5.69 | 1.93e-2 | -1.71 | |
| 3 | 0.999 | 23.21 | 9.69 | 1.21e-3 | -2.92 | 4.12 | 5.75e-2 | -1.24 | |
| 42day-stem | 1 | 1.000 | 18.02 | 6.33 | 1.24e-1 | -1.97 | 1.23 | 4.92e-1 | -0.39 |
| 2 | 0.997 | 18.97 | 6.42 | 1.17e-1 | -1.93 | 0.87 | 5.46e-1 | -0.26 | |
| 3 | 0.999 | 20.7 | 7.31 | 6.28e-1 | -2.22 | 1.77 | 4.97e-1 | -0.33 | |
| 53day-rosette | 1 | 1.000 | 29.71 | 15.66 | 1.93e-5 | -4.72 | 9.67 | 1.22e-3 | -2.91 |
| 2 | 1.000 | 24.22 | 11.93 | 2.57e-4 | -3.59 | 6.21 | 1.35e-2 | -1.87 | |
| 3 | 1.000 | 28.89 | 16 | 1.52e-5 | -4.82 | 9.35 | 1.54e-3 | -2.81 | |
| 53day-stem | 1 | 1.000 | 19.85 | 6.42 | 1.16e-2 | -1.93 | -0.09 | 1.07e+0 | 0.03 |
| 2 | 1.000 | 20.6 | 5.83 | 1.76e-2 | -1.75 | 1.18 | 4.43e-1 | -0.35 | |
| 3 | 0.999 | 22.92 | 5.31 | 2.51e-2 | -1.6 | 2.36 | 1.95e-1 | -0.71 | |
| 53day-inflores | 1 | 0.996 | 21.33 | 7.94 | 4.07e-3 | -2.39 | 2.79 | 1.45e-1 | -0.84 |
| 2 | 1.000 | 22.21 | 7.58 | 5.23e-3 | -2.28 | 3.02 | 1.23e-1 | -0.91 | |
| 3 | 0.995 | 20.84 | 7.01 | 7.73e-3 | -2.11 | 2.42 | 1.86e-1 | -0.73 | |
| 53day-silique | 1 | 1.000 | 29.39 | 9.54 | 1.34e-3 | -2.87 | 5.59 | 2.07e-2 | -1.68 |
| 2 | 1.000 | 30.96 | 11.67 | 3.07e-4 | -3.51 | 7.97 | 4.00e-3 | -2.4 | |
| 3 | 1.000 | 30.51 | 11.54 | 3.36e-4 | -3.47 | 7.81 | 4.47e-3 | -2.35 | |
Nothern hybridization
NGS expression data
Expression of miR319b transcript in NGS dataSequences
miRNA transcript
AGAGAGCUUUCUUCGGUCCACUCAUGGAGUAAUAUGUGAGAUUUAAUUGACUCUCGACUCAUUCAUCCAAAUACCAAAUGAAAGAAUUUGUUCUCAUAUGGUAAAUGAAUGAAUGAUGCGAGAGACAAAUUGAGUCUUCACUUCUCUAUGCUUGGACUGAAGGGAGCUCCCU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UUGGACUGAAGGGAGCUCCCU | miRNA | 152 | 172 | |
AGAGCTTTCTTCGGTCCACTCAT | primer | 5p | 3 | 25 |
CAATTTGTCTCTCGCATCATTCAT | primer | 3p | 109 | 132 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR319b found in PubMed |