Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 2.77405 | 0 | 3.00567 | 0 | |
| 10day-seedling | 3.93747 | 0.240137 | 5.74563 | 0.424542 | |
| 14day-seedling | 4.76334 | 0.376312 | 5.93656 | 0.48933 | |
| 25day-aerial | 4.61476 | 0.379774 | 6.397183 | 0.383555 | |
| 35day-aerial | 3.93676 | 0.637663 | 5.96642 | 0.336187 | |
| 42day-rosette | 3.17242 | 0.627833 | 5.15336 | 0.527195 | 42day-stem | 2.85213 | 0.359177 | 4.49559 | 0.398179 |
| 53day-rosette | 1.63898 | 0.190043 | 3.40076 | 0.248154 | 53day-stem | 3.68617 | 5.53411 | 5.10318 | 5.44743 | 53day-inflores | 2.10749 | 0.795276 | 3.54575 | 0.70415 | 53day-silique | 3.26488 | 0.814181 | 4.40747 | 0.853242 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 38.31 | 14.04 | 5.94e-5 | -4.23 | 13.27 | 1.01e-4 | -3.99 | |
| 10day-seedling | 1 | 1.000 | 23.86 | 11 | 4.89e-4 | -3.31 | 5.5 | 2.20e-2 | -1.66 |
| 2 | 1.000 | 23.36 | 10.11 | 9.02e-4 | -3.04 | 4.3 | 5.07e-2 | -1.29 | |
| 3 | 1.000 | 20.78 | 9.41 | 1.47e-3 | -2.83 | 2.69 | 1.55e-1 | -0.81 | |
| 14day-seedling | 1 | 1.000 | 21.98 | 8.4 | 2.95e-3 | -2.53 | 1.76 | 2.95e-1 | -0.53 |
| 2 | 0.982 | 25.97 | 7.86 | 4.31e-3 | -2.37 | 4.95 | 3.23e-2 | -1.49 | |
| 3 | 0.987 | 23.81 | 6.02 | 1.54e-2 | -1.81 | 3.88 | 6.78e-2 | -1.17 | |
| 25day-aerial | 1 | 0.998 | 21.91 | 9.05 | 1.89e-3 | -2.72 | 3.29 | 1.02e-1 | -0.99 |
| 2 | 0.998 | 22.2 | 8.16 | 3.50e-3 | -2.46 | 1.92 | 2.65e-1 | -0.58 | |
| 3 | 0.999 | 20.22 | 6.34 | 1.24e-2 | -1.91 | 0.77 | 5.85e-1 | -0.23 | |
| 35day-aerial | 1 | 0.999 | 24.68 | 10.23 | 8.33e-4 | -3.08 | 4.69 | 3.87e-2 | -1.41 |
| 2 | 0.999 | 21.42 | 8.7 | 2.41e-3 | -2.62 | 2.56 | 1.69e-1 | -0.77 | |
| 3 | 0.997 | 22.42 | 9.23 | 1.67e-3 | -2.78 | 3.04 | 1.21e-1 | -0.92 | |
| 42day-rosette | 1 | 0.999 | 28.66 | 15.12 | 2.80e-5 | -4.55 | 8.02 | 3.86e-3 | -2.41 |
| 2 | 0.998 | 25.9 | 11.52 | 3.40e-4 | -3.47 | 5.83 | 1.76e-2 | -1.75 | |
| 3 | 1.000 | 23.64 | 10.12 | 8.99e-4 | -3.05 | 4.55 | 4.26e-2 | -1.37 | |
| 42day-stem | 1 | 1.000 | 26.84 | 15.15 | 2.75e-1 | -4.56 | 9.84 | 1.93e-1 | -2.96 |
| 2 | 0.999 | 25.52 | 12.97 | 1.24e-1 | -3.95 | 7.42 | 5.82e-1 | -2.23 | |
| 3 | 1.000 | 26.44 | 13.55 | 1.18e-1 | -3.93 | 6.75 | 9.40e-1 | -2.32 | |
| 53day-rosette | 1 | 1.000 | 31.41 | 17.36 | 5.93e-6 | -5.23 | 11.37 | 3.77e-4 | -3.42 |
| 2 | 1.000 | 30.54 | 18.26 | 3.20e-6 | -5.5 | 12.54 | 1.68e-4 | -3.77 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | 1.000 | 38.25 | 24.82 | 3.37e-8 | -7.47 | 18.3 | 3.09e-6 | -5.51 |
| 2 | 0.000 | 4.92 | -9.86 | 9.27e+2 | 2.97 | -14.51 | 2.33e+4 | 4.37 | |
| 3 | 0.999 | 35.66 | 18.06 | 3.66e-6 | -5.44 | 15.1 | 2.84e-5 | -4.55 | |
| 53day-inflores | 1 | 0.999 | 32.33 | 18.94 | 1.99e-6 | -5.7 | 13.78 | 7.09e-5 | -4.15 |
| 2 | 0.997 | 30.72 | 16.09 | 1.44e-5 | -4.84 | 11.53 | 3.38e-4 | -3.47 | |
| 3 | 0.979 | 27.53 | 13.7 | 7.53e-5 | -4.12 | 9.11 | 1.81e-3 | -2.74 | |
| 53day-silique | 1 | 1.000 | 29.36 | 9.51 | 1.37e-3 | -2.86 | 5.56 | 2.12e-2 | -1.67 |
| 2 | 1.000 | 34.16 | 14.87 | 3.34e-5 | -4.48 | 11.16 | 4.36e-4 | -3.36 | |
| 3 | 1.000 | 31.81 | 12.84 | 1.36e-4 | -3.87 | 9.11 | 1.81e-3 | -2.74 | |
NGS expression data
Expression of miR169m transcript in NGS dataSequences
miRNA transcript
AUUUUCGCAGUUGGUUUCAAAAGUUUUUGUUAUAGUAUAUGAAAGUGAGUGUAUACAUUAUUUAGAAGGAGAAGUCAAAGAUGAAUAGAAGAAUCAUAUUUGGUAGCCAAGGAUGACUUGCCUGUUUCUUUGAGUAAAAUGGGUUAGUGUCAUGUUUGACAAGUGACUAUAAGUUAUAUCAAGCAAUGACCAUUUUACUCAUCAAAAGACAUCAGGCAGUCUCCUUGGCUAUCCUUAUAUGUUCUUCUCUCUCAUCUCAGACGUUUACCUUCAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAGCCAAGGAUGACUUGCCUG | miRNA | 104 | 124 | |
TTTTCGCAGTTGGTTTCAAAAGTT | primer | 5p | 2 | 25 |
GAAACAGGCAAGTCATCCTTGG | primer | 3p | 107 | 128 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169m found in PubMed |