Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 6.166637 | 0.186946 | 6.239149 | 0.325298 | |
| 10day-seedling | 4.27982 | 0.386765 | 6.087975 | 0.236611 | |
| 14day-seedling | 4.83801 | 0.710548 | 6.011367 | 0.238657 | |
| 25day-aerial | 4.55936 | 0.161293 | 6.321891 | 0.0683132 | |
| 35day-aerial | 4.52472 | 0.270671 | 6.321975 | 0.203927 | |
| 42day-rosette | 4.68121 | 0.158288 | 6.525383 | 0.134632 | 42day-stem | 4.60828 | 0.121265 | 6.33079 | 0.137401 |
| 53day-rosette | 4.99508 | 0.233716 | 6.83758 | 0.185551 | 53day-stem | 4.84628 | 0.583787 | 6.321661 | 0.0695445 | 53day-inflores | 4.81342 | 0.0751395 | 6.307023 | 0.0564237 | 53day-silique | 4.92439 | 0.119485 | 6.066987 | 0.0790697 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 26.62 | 2.77 | 1.47e-1 | -0.83 | 2.68 | 1.56e-1 | -0.81 |
| 2 | 1.000 | 31.37 | 3.39 | 9.54e-2 | -1.02 | 3.52 | 8.69e-2 | -1.06 | |
| 3 | 1.000 | 26.42 | 2.15 | 2.26e-1 | -0.65 | 1.38 | 3.84e-1 | -0.42 | |
| 10day-seedling | 1 | 1.000 | 20.48 | 7.62 | 5.09e-3 | -2.29 | 2.13 | 2.29e-1 | -0.64 |
| 2 | 1.000 | 22.61 | 9.36 | 1.52e-3 | -2.82 | 3.55 | 8.54e-2 | -1.07 | |
| 3 | 1.000 | 21.49 | 10.13 | 8.95e-4 | -3.05 | 3.41 | 9.39e-2 | -1.03 | |
| 14day-seedling | 1 | 0.998 | 22.9 | 9.33 | 1.56e-3 | -2.81 | 2.68 | 1.56e-1 | -0.81 |
| 2 | 0.996 | 25.21 | 7.1 | 7.30e-3 | -2.14 | 4.19 | 5.46e-2 | -1.26 | |
| 3 | 0.996 | 22.65 | 4.86 | 3.43e-2 | -1.46 | 2.73 | 1.51e-1 | -0.82 | |
| 25day-aerial | 1 | 0.996 | 21 | 8.15 | 3.53e-3 | -2.45 | 2.39 | 1.91e-1 | -0.72 |
| 2 | 0.998 | 22.67 | 8.62 | 2.54e-3 | -2.6 | 2.38 | 1.92e-1 | -0.72 | |
| 3 | 0.999 | 21.43 | 7.55 | 5.32e-3 | -2.27 | 1.99 | 2.52e-1 | -0.6 | |
| 35day-aerial | 1 | 0.999 | 21.77 | 7.31 | 6.28e-3 | -2.2 | 1.78 | 2.92e-1 | -0.54 |
| 2 | 1.000 | 21.88 | 9.16 | 1.75e-3 | -2.76 | 3.03 | 1.23e-1 | -0.91 | |
| 3 | 0.999 | 21.33 | 8.14 | 3.55e-3 | -2.45 | 1.95 | 2.59e-1 | -0.59 | |
| 42day-rosette | 1 | 0.998 | 21.81 | 8.27 | 3.25e-3 | -2.49 | 1.16 | 4.47e-1 | -0.35 |
| 2 | 0.999 | 21.59 | 7.21 | 6.75e-3 | -2.17 | 1.52 | 3.49e-1 | -0.46 | |
| 3 | 0.998 | 21.14 | 7.62 | 5.09e-3 | -2.29 | 2.05 | 2.42e-1 | -0.62 | |
| 42day-stem | 1 | 0.998 | 19.75 | 8.66 | 3.75e-1 | -2.43 | 2.75 | 1.49e-1 | -0.83 |
| 2 | 0.999 | 20.04 | 7.5 | 5.53e-1 | -2.26 | 1.95 | 2.59e-1 | -0.59 | |
| 3 | 1.000 | 21.66 | 8.28 | 3.22e-1 | -2.49 | 1.98 | 2.56e-1 | -0.59 | |
| 53day-rosette | 1 | 1.000 | 19.89 | 5.84 | 1.75e-2 | -1.76 | -0.15 | 1.11e+0 | 0.04 |
| 2 | 1.000 | 19.04 | 6.76 | 9.25e-3 | -2.03 | 1.04 | 4.86e-1 | -0.31 | |
| 3 | 1.000 | 20.28 | 7.38 | 5.99e-3 | -2.22 | 0.73 | 6.04e-1 | -0.22 | |
| 53day-stem | 1 | 0.999 | 22.45 | 9.03 | 1.92e-3 | -2.72 | 2.51 | 1.76e-1 | -0.75 |
| 2 | 1.000 | 21.48 | 6.71 | 9.57e-3 | -2.02 | 2.05 | 2.41e-1 | -0.62 | |
| 3 | 1.000 | 22.76 | 5.15 | 2.81e-2 | -1.55 | 2.2 | 2.18e-1 | -0.66 | |
| 53day-inflores | 1 | 0.999 | 20.91 | 7.51 | 5.47e-3 | -2.26 | 2.36 | 1.95e-1 | -0.71 |
| 2 | 1.000 | 21.63 | 7 | 7.79e-3 | -2.11 | 2.45 | 1.83e-1 | -0.74 | |
| 3 | 0.999 | 20.51 | 6.68 | 9.75e-3 | -2.01 | 2.09 | 2.35e-1 | -0.63 | |
| 53day-silique | 1 | 1.000 | 27.2 | 7.35 | 6.12e-3 | -2.21 | 3.4 | 9.46e-2 | -1.02 |
| 2 | 1.000 | 25.95 | 6.66 | 9.92e-3 | -2 | 2.95 | 1.29e-1 | -0.89 | |
| 3 | 1.000 | 25.65 | 6.68 | 9.77e-3 | -2.01 | 2.94 | 1.30e-1 | -0.89 | |
NGS expression data
Expression of miR168a transcript in NGS dataSequences
miRNA transcript
CACCAUCGGGCUCGGAUUCGCUUGGUGCAGGUCGGGAACCAAUUCGGCUGACACAGCCUCGUGACUUUUAAACCUUUAUUGGUUUGUGAGCAGGGAUUGGAUCCCGCCUUGCAUCAACUGAAUCGGAUCCUCGAGGUG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGCUUGGUGCAGGUCGGGAA | miRNA | 18 | 38 | |
ATTCGCTTGGTGCAGGTC | primer | 5p | 16 | 33 |
TCCGATTCAGTTGATGCAAG | primer | 3p | 108 | 127 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR168a found in PubMed |