Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 6.66532 | 0.435727 | 6.737832 | 0.560851 | |
| 10day-seedling | 5.39288 | 0.467439 | 7.201035 | 0.36764 | |
| 14day-seedling | 4.61643 | 0.410938 | 6.073927 | 1.5553 | |
| 25day-aerial | 4.67741 | 0.177226 | 6.439964 | 0.122879 | |
| 35day-aerial | 5.15946 | 0.23385 | 6.9733892 | 0.921558 | |
| 42day-rosette | 5.39184 | 0.334251 | 7.234489 | 0.429203 | 42day-stem | 5.61727 | 0.195517 | 7.341614 | 0.1923 |
| 53day-rosette | 6.183924 | 0.221595 | 8.02643 | 0.0891211 | 53day-stem | 5.5038 | 0.607477 | 7.198023 | 0.431397 | 53day-inflores | 5.70032 | 0.34377 | 7.0284483 | 0.526339 | 53day-silique | 6.122001 | 0.151731 | 7.264595 | 0.119551 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 24.52 | 0.66 | 6.33e-1 | -0.2 | 0.57 | 6.72e-1 | -0.17 |
| 2 | 1.000 | 30.71 | 2.73 | 1.51e-1 | -0.82 | 2.87 | 1.37e-1 | -0.86 | |
| 3 | 1.000 | 24.22 | -0.06 | 1.04e+0 | 0.02 | -0.83 | 1.77e+0 | 0.25 | |
| 10day-seedling | 1 | 1.000 | 16.41 | 3.55 | 8.55e-2 | -1.07 | -1.95 | 3.85e+0 | 0.59 |
| 2 | 1.000 | 19.55 | 6.3 | 1.27e-2 | -1.9 | 0.49 | 7.14e-1 | -0.15 | |
| 3 | 1.000 | 17.54 | 6.17 | 1.39e-2 | -1.86 | -0.54 | 1.46e+0 | 0.16 | |
| 14day-seedling | 1 | 1.000 | 19.93 | 6.36 | 1.22e-2 | -1.91 | -0.29 | 1.22e+0 | 0.09 |
| 2 | 0.979 | 26.58 | 8.47 | 2.81e-3 | -2.55 | 5.57 | 2.11e-2 | -1.68 | |
| 3 | 0.995 | 26.45 | 8.66 | 2.47e-3 | -2.61 | 6.52 | 1.09e-2 | -1.96 | |
| 25day-aerial | 1 | 0.998 | 20.9 | 8.05 | 3.79e-3 | -2.42 | 2.29 | 2.05e-1 | -0.69 |
| 2 | 0.998 | 22.11 | 8.07 | 3.73e-3 | -2.43 | 1.82 | 2.83e-1 | -0.55 | |
| 3 | 1.000 | 20.92 | 7.04 | 7.62e-3 | -2.12 | 1.47 | 3.60e-1 | -0.44 | |
| 35day-aerial | 1 | 0.999 | 19.68 | 5.22 | 2.68e-2 | -1.57 | -0.32 | 1.24e+0 | 0.1 |
| 2 | 0.998 | 19.35 | 6.63 | 1.01e-2 | -2 | 0.5 | 7.08e-1 | -0.15 | |
| 3 | 1.000 | 19.67 | 6.48 | 1.12e-2 | -1.95 | 0.29 | 8.17e-1 | -0.09 | |
| 42day-rosette | 1 | 0.999 | 18.93 | 5.39 | 2.39e-2 | -1.62 | -1.72 | 3.29e+0 | 0.52 |
| 2 | 1.000 | 18.59 | 4.21 | 5.41e-2 | -1.27 | -1.48 | 2.79e+0 | 0.45 | |
| 3 | 0.999 | 19.95 | 6.43 | 1.16e-2 | -1.94 | 0.86 | 5.51e-1 | -0.26 | |
| 42day-stem | 1 | 1.000 | 16.55 | 4.87 | 3.44e-1 | -1.46 | -0.45 | 1.37e+0 | 0.14 |
| 2 | 0.996 | 16.4 | 3.85 | 6.92e-1 | -1.16 | -1.7 | 3.24e+0 | 0.52 | |
| 3 | 1.000 | 18.45 | 5.67 | 2.99e-1 | -1.53 | -1.25 | 2.36e+0 | 0.37 | |
| 53day-rosette | 1 | 1.000 | 16.78 | 2.73 | 1.51e-1 | -0.82 | -3.26 | 9.57e+0 | 0.98 |
| 2 | 1.000 | 14.25 | 1.97 | 2.56e-1 | -0.59 | -3.75 | 1.35e+1 | 1.13 | |
| 3 | 1.000 | 16.33 | 3.44 | 9.23e-2 | -1.03 | -3.22 | 9.32e+0 | 0.97 | |
| 53day-stem | 1 | 1.000 | 19.5 | 6.08 | 1.48e-2 | -1.83 | -0.44 | 1.35e+0 | 0.13 |
| 2 | 1.000 | 17.42 | 2.64 | 1.60e-1 | -0.79 | -2.01 | 4.04e+0 | 0.61 | |
| 3 | 1.000 | 21.21 | 3.61 | 8.21e-2 | -1.09 | 0.65 | 6.36e-1 | -0.2 | |
| 53day-inflores | 1 | 0.993 | 18.73 | 5.33 | 2.48e-2 | -1.61 | 0.18 | 8.83e-1 | -0.05 |
| 2 | 1.000 | 18.84 | 4.21 | 5.39e-2 | -1.27 | -0.34 | 1.27e+0 | 0.1 | |
| 3 | 1.000 | 17.06 | 3.23 | 1.07e-1 | -0.97 | -1.36 | 2.57e+0 | 0.41 | |
| 53day-silique | 1 | 1.000 | 23.22 | 3.37 | 9.66e-2 | -1.02 | -0.58 | 1.49e+0 | 0.17 |
| 2 | 1.000 | 21.67 | 2.38 | 1.93e-1 | -0.72 | -1.33 | 2.51e+0 | 0.4 | |
| 3 | 1.000 | 21.97 | 3 | 1.25e-1 | -0.9 | -0.73 | 1.66e+0 | 0.22 | |
NGS expression data
Expression of miR860 transcript in NGS dataSequences
miRNA transcript
UCUCCCCAAAGAUGUUCCAUGCGUUCCGGAACCUAUAGAAUCUCGUUGGUUUUGUGUUAAGUCUUUUCUUGGAGAAAUGUCUUGGUUAAAUUCAAUAUAUAUAGUCCAAUCUAUUGAAGUACUAGUACACCAGCUCUAAUAAGCUGAUGUGGGUAAGUAGUUCAAUAGAUUGGACUAUGUAUAUUAAAUUUGACUAAGAGAUAAACUAGAGAAUGGGAAUAAAGAAUGAGAAACGGU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCAAUAGAUUGGACUAUGUAU | miRNA | 162 | 182 | |
CTCCCCAAAGATGTTCCATGC | primer | 5p | 2 | 22 |
CCGTTTCTCATTCTTTATTCCCATTC | primer | 3p | 211 | 236 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR860 found in PubMed |