Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.29973 | 1.27562 | 4.37225 | 1.36362 | |
| 10day-seedling | 3.10499 | 0.32244 | 4.91315 | 0.214863 | |
| 14day-seedling | 3.57373 | 0.420889 | 4.74674 | 0.385003 | |
| 25day-aerial | 3.25116 | 0.154105 | 5.0317 | 0.15944 | |
| 35day-aerial | 3.13119 | 0.312867 | 4.87492 | 0.228043 | |
| 42day-rosette | 3.14309 | 0.271203 | 4.71375 | 0.335743 | 42day-stem | 3.36084 | 0.224053 | 5.08334 | 0.335994 |
| 53day-rosette | 3.62598 | 0.467977 | 5.46848 | 0.42919 | 53day-stem | 3.57506 | 0.299298 | 5.01913 | 0.215911 | 53day-inflores | 3.39105 | 0.260874 | 5.00495 | 0.323331 | 53day-silique | 4.12039 | 0.288689 | 5.26299 | 0.328876 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 29.84 | 5.98 | 1.58e-2 | -1.8 | 5.9 | 1.68e-2 | -1.77 |
| 2 | 1.000 | 41.8 | 13.82 | 6.92e-5 | -4.16 | 13.95 | 6.30e-5 | -4.2 | |
| 3 | 1.000 | 31.38 | 7.11 | 7.25e-3 | -2.14 | 6.34 | 1.24e-2 | -1.91 | |
| 10day-seedling | 1 | 1.000 | 24.57 | 11.71 | 2.99e-4 | -3.52 | 6.21 | 1.35e-2 | -1.87 |
| 2 | 1.000 | 26.7 | 13.45 | 8.91e-5 | -4.05 | 7.64 | 5.01e-3 | -2.3 | |
| 3 | 1.000 | 25.02 | 13.66 | 7.75e-5 | -4.11 | 6.94 | 8.13e-3 | -2.09 | |
| 14day-seedling | 1 | 1.000 | 26.3 | 12.72 | 1.48e-4 | -3.83 | 6.08 | 1.48e-2 | -1.83 |
| 2 | 0.999 | 29.59 | 11.48 | 3.50e-4 | -3.46 | 8.58 | 2.62e-3 | -2.58 | |
| 3 | 0.999 | 27.73 | 9.94 | 1.02e-3 | -2.99 | 7.8 | 4.50e-3 | -2.35 | |
| 25day-aerial | 1 | 1.000 | 24.72 | 11.86 | 2.68e-4 | -3.57 | 6.1 | 1.45e-2 | -1.84 |
| 2 | 0.992 | 26.67 | 12.63 | 1.58e-4 | -3.8 | 6.38 | 1.20e-2 | -1.92 | |
| 3 | 0.999 | 26.57 | 12.69 | 1.51e-4 | -3.82 | 7.13 | 7.15e-3 | -2.15 | |
| 35day-aerial | 1 | 1.000 | 26.25 | 11.79 | 2.82e-4 | -3.55 | 6.26 | 1.31e-2 | -1.88 |
| 2 | 1.000 | 26.6 | 13.88 | 6.65e-5 | -4.18 | 7.74 | 4.67e-3 | -2.33 | |
| 3 | 0.999 | 26.08 | 12.88 | 1.32e-4 | -3.88 | 6.69 | 9.65e-3 | -2.02 | |
| 42day-rosette | 1 | 1.000 | 27.38 | 13.84 | 6.80e-5 | -4.17 | 6.74 | 9.37e-3 | -2.03 |
| 2 | 1.000 | 26.56 | 12.17 | 2.16e-4 | -3.67 | 6.48 | 1.12e-2 | -1.95 | |
| 3 | 0.999 | 25.94 | 12.42 | 1.83e-4 | -3.74 | 6.85 | 8.66e-3 | -2.06 | |
| 42day-stem | 1 | 0.996 | 23.57 | 11.88 | 2.65e-1 | -3.58 | 6.57 | 1.50e-1 | -1.98 |
| 2 | 0.999 | 25.46 | 12.91 | 1.30e-1 | -3.89 | 7.37 | 6.64e-1 | -2.22 | |
| 3 | 1.000 | 24.85 | 11.47 | 3.53e-1 | -3.45 | 5.16 | 2.79e-1 | -1.55 | |
| 53day-rosette | 1 | 1.000 | 23.49 | 9.44 | 1.44e-3 | -2.84 | 3.46 | 9.12e-2 | -1.04 |
| 2 | 1.000 | 24.09 | 11.8 | 2.80e-4 | -3.55 | 6.09 | 1.47e-2 | -1.83 | |
| 3 | 1.000 | 25.27 | 12.38 | 1.88e-4 | -3.73 | 5.72 | 1.90e-2 | -1.72 | |
| 53day-stem | 1 | 1.000 | 25.92 | 12.5 | 1.73e-4 | -3.76 | 5.98 | 1.59e-2 | -1.8 |
| 2 | 1.000 | 25.82 | 11.04 | 4.74e-4 | -3.32 | 6.39 | 1.19e-2 | -1.92 | |
| 3 | 1.000 | 27.93 | 10.33 | 7.78e-4 | -3.11 | 7.37 | 6.03e-3 | -2.22 | |
| 53day-inflores | 1 | 1.000 | 26.27 | 12.88 | 1.33e-4 | -3.88 | 7.72 | 4.74e-3 | -2.32 |
| 2 | 0.999 | 25.77 | 11.14 | 4.42e-4 | -3.35 | 6.59 | 1.04e-2 | -1.98 | |
| 3 | 0.999 | 23.99 | 10.16 | 8.72e-4 | -3.06 | 5.57 | 2.10e-2 | -1.68 | |
| 53day-silique | 1 | 1.000 | 28.34 | 8.49 | 2.77e-3 | -2.56 | 4.54 | 4.29e-2 | -1.37 |
| 2 | 1.000 | 29.63 | 10.34 | 7.71e-4 | -3.11 | 6.64 | 1.00e-2 | -2 | |
| 3 | 1.000 | 28.83 | 9.86 | 1.07e-3 | -2.97 | 6.13 | 1.43e-2 | -1.85 | |
NGS expression data
Expression of miR823 transcript in NGS dataSequences
miRNA transcript
AAUUUCAGAGAUACUAAUCCAAGAGAUGGCGGAUACGUUUUACAAUCACGAAUCUUGUAUGAUCACUAACCAUUGGAACAAUAAGAGUAUAUAAUGAGUAUAUCACCAUUUAGUUCUAGUGGGUGGUGAUCAUAUAAGAUUGGUAAUGGUAACAGUGAAACAUAUCCCCCAUCUAUUGGGAUUAAUUAGUC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGGGUGGUGAUCAUAUAAGAU | miRNA | 120 | 140 | |
AGAGATGGCGGATACGTTTTACAA | primer | 5p | 22 | 45 |
ACCAATCTTATATGATCACCACCCACTA | primer | 3p | 117 | 144 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR823 found in PubMed |