Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.97481 | 0.221938 | 4.04732 | 0.260232 | |
| 10day-seedling | 2.80047 | 0.951718 | 4.60863 | 0.817635 | |
| 14day-seedling | 2.27828 | 0.660361 | 3.71383 | 0.138107 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | 0.62378 | 0.618949 | 2.50075 | 0.37348 | |
| 42day-rosette | 1.42978 | 0.120551 | 3.16225 | 0.148813 | 42day-stem | 0.1642 | 0.417495 | 2.18004 | 0.413994 |
| 53day-rosette | 0.84754 | 0.956034 | 2.69004 | 0.831558 | 53day-stem | 1.50884 | 1.63826 | 2.65381 | 1.27661 | 53day-inflores | 1.48217 | 0.460501 | 2.91573 | 0.405991 | 53day-silique | 3.47802 | 0.580763 | 4.6302 | 0.633137 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.72 | 10.86 | 5.38e-4 | -3.27 | 10.77 | 5.72e-4 | -3.24 |
| 2 | 1.000 | 37.4 | 9.42 | 1.46e-3 | -2.83 | 9.55 | 1.33e-3 | -2.88 | |
| 3 | 1.000 | 34.14 | 9.87 | 1.07e-3 | -2.97 | 9.1 | 1.82e-3 | -2.74 | |
| 10day-seedling | 1 | 1.000 | 23.17 | 10.31 | 7.87e-4 | -3.1 | 4.82 | 3.55e-2 | -1.45 |
| 2 | 1.000 | 28.78 | 15.53 | 2.12e-5 | -4.67 | 9.71 | 1.19e-3 | -2.92 | |
| 3 | 1.000 | 27.38 | 16.01 | 1.51e-5 | -4.82 | 9.3 | 1.59e-3 | -2.8 | |
| 14day-seedling | 1 | 0.993 | 30.81 | 17.24 | 6.48e-6 | -5.19 | 10.59 | 6.48e-4 | -3.19 |
| 2 | 0.990 | 32.24 | 14.13 | 5.56e-5 | -4.25 | 11.23 | 4.17e-4 | -3.38 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.999 | 32.92 | 20.2 | 8.29e-7 | -6.08 | 14.07 | 5.82e-5 | -4.24 | |
| 3 | 1.000 | 32.92 | 19.73 | 1.15e-6 | -5.94 | 13.54 | 8.40e-5 | -4.08 | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.997 | 33.17 | 18.79 | 2.21e-6 | -5.66 | 13.1 | 1.14e-4 | -3.94 | |
| 3 | 0.997 | 31.49 | 17.97 | 3.90e-6 | -5.41 | 12.4 | 1.85e-4 | -3.73 | |
| 42day-stem | 1 | 1.000 | 32.64 | 2.95 | 4.94e-7 | -6.36 | 15.64 | 1.96e-1 | -4.78 |
| 2 | 0.999 | 32.8 | 2.26 | 7.96e-1 | -6.99 | 14.71 | 3.73e-1 | -4.43 | |
| 3 | 1.000 | 37.14 | 23.76 | 7.46e-8 | -7.15 | 17.45 | 5.58e-6 | -5.25 | |
| 53day-rosette | 1 | 1.000 | 32 | 17.96 | 3.93e-6 | -5.41 | 11.97 | 2.50e-4 | -3.6 |
| 2 | 1.000 | 31.63 | 19.34 | 1.50e-6 | -5.82 | 13.63 | 7.91e-5 | -4.1 | |
| 3 | 1.000 | 36.91 | 24.02 | 5.89e-8 | -7.23 | 17.36 | 5.95e-6 | -5.23 | |
| 53day-stem | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 36.86 | 22.09 | 2.24e-7 | -6.65 | 17.44 | 5.64e-6 | -5.25 | |
| 3 | 1.000 | 32 | 14.39 | 4.65e-5 | -4.33 | 11.44 | 3.60e-4 | -3.44 | |
| 53day-inflores | 1 | 1.000 | 32.9 | 19.51 | 1.34e-6 | -5.87 | 14.35 | 4.78e-5 | -4.32 |
| 2 | 0.999 | 33.51 | 18.88 | 2.07e-6 | -5.68 | 14.33 | 4.87e-5 | -4.31 | |
| 3 | 0.998 | 30.43 | 16.6 | 1.01e-5 | -5 | 12.01 | 2.42e-4 | -3.62 | |
| 53day-silique | 1 | 1.000 | 30.18 | 10.34 | 7.74e-4 | -3.11 | 6.39 | 1.20e-2 | -1.92 |
| 2 | 1.000 | 32.36 | 13.06 | 1.17e-4 | -3.93 | 9.36 | 1.52e-3 | -2.82 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
NGS expression data
Expression of miR399f transcript in NGS dataSequences
miRNA transcript
AUAUGCAUUACAGGGCAAGAUCACCAUUGGCAGAGAUCUAUUACUUCAUUCUUGCAUCAUAUGCAUAAAUGUUUGUGGUGAGCUCUCUGCCAAAGGAGAUUUGCCCGGUAAUUCUCUUGCGC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCAAAGGAGAUUUGCCCGG | miRNA | 88 | 108 | |
GCATTACAGGGCAAGATCACCATTGG | primer | 5p | 5 | 30 |
GCGCAAGAGAATTACCGGGCAAATC | primer | 3p | 98 | 122 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR399f found in PubMed |