Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | N/D | - | N/D | - | |
| 10day-seedling | 2.35529 | 0.478972 | 4.16345 | 0.316409 | |
| 14day-seedling | 2.18596 | 0.0422068 | 3.66131 | 0.699975 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | N/D | - | N/D | - | |
| 42day-rosette | 1.50336 | 0 | 3.3037 | 0 | 42day-stem | -1.17473 | 0 | 0.58102 | 0 |
| 53day-rosette | N/D | - | N/D | - | 53day-stem | N/D | - | N/D | - | 53day-inflores | 0.50241 | 0.394363 | 1.85636 | 0.432636 | 53day-silique | 3.5634 | 0.832163 | 4.71558 | 0.884537 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 10day-seedling | 1 | 1.000 | 26.57 | 13.71 | 7.46e-5 | -4.13 | 8.22 | 3.36e-3 | -2.47 |
| 2 | 1.000 | 28.98 | 15.73 | 1.84e-5 | -4.73 | 9.92 | 1.04e-3 | -2.98 | |
| 3 | 1.000 | 28.22 | 16.85 | 8.47e-6 | -5.07 | 10.14 | 8.88e-4 | -3.05 | |
| 14day-seedling | 1 | 0.999 | 29.66 | 16.09 | 1.43e-5 | -4.84 | 9.45 | 1.43e-3 | -2.84 |
| 2 | 0.973 | 33.75 | 15.64 | 1.96e-5 | -4.71 | 12.74 | 1.47e-4 | -3.83 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 32.35 | 17.97 | 3.89e-6 | -5.41 | 12.28 | 2.01e-4 | -3.7 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-stem | 1 | 1.000 | 38.32 | 26.63 | 9.66e-9 | -8.17 | 21.32 | 3.82e-7 | -6.42 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-inflores | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.989 | 37.14 | 22.51 | 1.67e-7 | -6.78 | 17.95 | 3.94e-6 | -5.4 | |
| 3 | 0.997 | 34.49 | 20.66 | 6.04e-7 | -6.22 | 16.07 | 1.46e-5 | -4.84 | |
| 53day-silique | 1 | 1.000 | 29.31 | 9.46 | 1.42e-3 | -2.85 | 5.51 | 2.19e-2 | -1.66 |
| 2 | 1.000 | 32.66 | 13.37 | 9.44e-5 | -4.03 | 9.67 | 1.23e-3 | -2.91 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
NGS expression data
Expression of miR399e transcript in NGS dataSequences
miRNA transcript
GCAAUAAAGUGAGAGAGGAAAACUGAGAUUAUUUCUUGGUAGAAAGCAUUACAGGGCGAAUCCUCUAUUGGCAGUGGAAGUUGAUGACCCUUAUAUGUUAUUUUCUCAUCAUUUUCCUCUGCCAAAGGAGAUUUGCCUCGCAAUGCUUCA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCAAAGGAGAUUUGCCUCG | miRNA | 120 | 140 | |
GCAATAAAGTGAGAGAGGAAAACTGAGA | primer | 5p | 1 | 28 |
GCAGAGGAAAATGATGAGAAAATAAC | primer | 3p | 97 | 122 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR399e found in PubMed |