Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.1904 | 0.787651 | 4.26291 | 0.857666 | |
| 10day-seedling | 3.66417 | 0.947388 | 5.47232 | 0.822877 | |
| 14day-seedling | 2.76568 | 0.302011 | 3.93873 | 0.540226 | |
| 25day-aerial | 2.62915 | 0.239693 | 4.4003 | 0.310713 | |
| 35day-aerial | 2.40851 | 0.699773 | 4.30906 | 0.449712 | |
| 42day-rosette | 2.75593 | 1.11387 | 4.75763 | 0.581839 | 42day-stem | 2.3253 | 0.47547 | 4.10654 | 0.521086 |
| 53day-rosette | 2.59951 | 0.129303 | 4.44201 | 0.0177272 | 53day-stem | 2.06718 | 0.438912 | 3.48463 | 0.310799 | 53day-inflores | 2.59639 | 0.098379 | 3.83312 | 0.356877 | 53day-silique | 3.35776 | 0.287342 | 4.50036 | 0.327839 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 36.18 | 12.32 | 1.96e-4 | -3.71 | 12.23 | 2.08e-4 | -3.68 |
| 2 | 1.000 | 36.22 | 8.24 | 3.31e-3 | -2.48 | 8.37 | 3.02e-3 | -2.52 | |
| 3 | 1.000 | 31.71 | 7.44 | 5.75e-3 | -2.24 | 6.67 | 9.81e-3 | -2.01 | |
| 10day-seedling | 1 | 1.000 | 20.31 | 7.45 | 5.73e-3 | -2.24 | 1.95 | 2.58e-1 | -0.59 |
| 2 | 1.000 | 26.1 | 12.86 | 1.35e-4 | -3.87 | 7.04 | 7.58e-3 | -2.12 | |
| 3 | 1.000 | 24.31 | 12.94 | 1.27e-4 | -3.9 | 6.23 | 1.33e-2 | -1.87 | |
| 14day-seedling | 1 | 0.998 | 28.36 | 14.79 | 3.52e-5 | -4.45 | 8.15 | 3.52e-3 | -2.45 |
| 2 | 0.000 | 32.59 | 14.48 | 4.37e-5 | -4.36 | 11.58 | 3.27e-4 | -3.48 | |
| 3 | 0.998 | 30.71 | 12.92 | 1.29e-4 | -3.89 | 10.78 | 5.68e-4 | -3.25 | |
| 25day-aerial | 1 | 0.999 | 28.23 | 15.38 | 2.35e-5 | -4.63 | 9.62 | 1.27e-3 | -2.9 |
| 2 | 0.989 | 27.85 | 13.8 | 7.00e-5 | -4.15 | 7.56 | 5.30e-3 | -2.28 | |
| 3 | 0.998 | 28.17 | 14.29 | 4.98e-5 | -4.3 | 8.73 | 2.35e-3 | -2.63 | |
| 35day-aerial | 1 | 0.996 | 27.68 | 13.23 | 1.04e-4 | -3.98 | 7.69 | 4.84e-3 | -2.32 |
| 2 | 1.000 | 29.45 | 16.73 | 9.21e-6 | -5.04 | 10.59 | 6.47e-4 | -3.19 | |
| 3 | 0.000 | 27.91 | 14.72 | 3.71e-5 | -4.43 | 8.53 | 2.70e-3 | -2.57 | |
| 42day-rosette | 1 | 0.995 | 30.32 | 16.79 | 8.85e-6 | -5.05 | 9.68 | 1.22e-3 | -2.91 |
| 2 | 0.999 | 26.43 | 12.05 | 2.36e-4 | -3.63 | 6.36 | 1.22e-2 | -1.91 | |
| 3 | 0.998 | 25.4 | 11.88 | 2.66e-4 | -3.58 | 6.31 | 1.26e-2 | -1.9 | |
| 42day-stem | 1 | 0.995 | 28.37 | 16.68 | 9.55e-6 | -5.2 | 11.37 | 3.79e-1 | -3.42 |
| 2 | 0.999 | 27.66 | 15.12 | 2.82e-1 | -4.56 | 9.57 | 1.32e-1 | -2.88 | |
| 3 | 0.999 | 27.57 | 14.18 | 5.38e-1 | -4.27 | 7.88 | 4.26e-1 | -2.38 | |
| 53day-rosette | 1 | 1.000 | 28.57 | 14.52 | 4.24e-5 | -4.37 | 8.54 | 2.69e-3 | -2.57 |
| 2 | 1.000 | 26.53 | 14.24 | 5.16e-5 | -4.29 | 8.53 | 2.71e-3 | -2.57 | |
| 3 | 1.000 | 27.98 | 15.09 | 2.87e-5 | -4.54 | 8.43 | 2.90e-3 | -2.54 | |
| 53day-stem | 1 | 1.000 | 30.55 | 17.13 | 6.98e-6 | -5.16 | 10.61 | 6.40e-4 | -3.19 |
| 2 | 0.999 | 32.1 | 17.32 | 6.10e-6 | -5.21 | 12.67 | 1.53e-4 | -3.81 | |
| 3 | 0.996 | 32.31 | 14.71 | 3.74e-5 | -4.43 | 11.75 | 2.90e-4 | -3.54 | |
| 53day-inflores | 1 | 1.000 | 28.3 | 14.9 | 3.26e-5 | -4.49 | 9.75 | 1.16e-3 | -2.94 |
| 2 | 1.000 | 29.34 | 14.71 | 3.72e-5 | -4.43 | 10.16 | 8.75e-4 | -3.06 | |
| 3 | 0.993 | 28.08 | 14.25 | 5.12e-5 | -4.29 | 9.66 | 1.23e-3 | -2.91 | |
| 53day-silique | 1 | 1.000 | 30.86 | 11.01 | 4.84e-4 | -3.32 | 7.06 | 7.48e-3 | -2.13 |
| 2 | 1.000 | 32.1 | 12.81 | 1.40e-4 | -3.86 | 9.1 | 1.82e-3 | -2.74 | |
| 3 | 1.000 | 31.45 | 12.48 | 1.75e-4 | -3.76 | 8.75 | 2.33e-3 | -2.63 | |
NGS expression data
Expression of miR399c transcript in NGS dataSequences
miRNA transcript
GCGGAGCAGUAAUAGGGCAUCUUUCUAUUGGCAGGCGACUUGGCUAUUUGUAUCUUUUGUGUUCUUGACUAUUGGCUAUGUCACUUGCCAAAGGAGAGUUGCCCUGUCACUGCUUCCGCUUAAACACA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCAAAGGAGAGUUGCCCUG | miRNA | 86 | 106 | |
GCGGAGCAGTAATAGGGCATCT | primer | 5p | 1 | 22 |
TGTGTTTAAGCGGAAGCAGTGA | primer | 3p | 107 | 128 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR399c found in PubMed |