Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
Read more
Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.6784 | 0.206933 | 3.77407 | 0.39919 | |
| 10day-seedling | 2.08515 | 0.746977 | 3.89331 | 0.561135 | |
| 14day-seedling | 2.58173 | 0.297697 | 3.75487 | 0.943969 | |
| 25day-aerial | 1.25537 | 0.522272 | 3.03222 | 0.666922 | |
| 35day-aerial | 1.70999 | 0.241307 | 3.49078 | 0.203629 | |
| 42day-rosette | 1.35765 | 0.954225 | 3.36157 | 0.72665 | 42day-stem | 1.46322 | 0.0830085 | 3.19762 | 0.0725432 |
| 53day-rosette | 2.37692 | 0.186424 | 4.21942 | 0.272878 | 53day-stem | 2.24302 | 1.36583 | 3.36462 | 0.865188 | 53day-inflores | 1.9749 | 0.612141 | 3.4074 | 0.508873 | 53day-silique | 3.68024 | 0.810319 | 4.82283 | 0.847727 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 39.5 | 11.52 | 3.40e-4 | -3.47 | 11.65 | 3.10e-4 | -3.51 | |
| 3 | 1.000 | 34.82 | 10.55 | 6.68e-4 | -3.18 | 9.78 | 1.14e-3 | -2.94 | |
| 10day-seedling | 1 | 1.000 | 26.83 | 13.97 | 6.23e-5 | -4.21 | 8.48 | 2.81e-3 | -2.55 |
| 2 | 1.000 | 29.34 | 16.09 | 1.43e-5 | -4.84 | 10.28 | 8.05e-4 | -3.09 | |
| 3 | 1.000 | 30.29 | 18.92 | 2.02e-6 | -5.69 | 12.2 | 2.12e-4 | -3.67 | |
| 14day-seedling | 1 | 1.000 | 27.67 | 14.09 | 5.72e-5 | -4.24 | 7.45 | 5.72e-3 | -2.24 |
| 2 | 0.998 | 32.23 | 14.12 | 5.63e-5 | -4.25 | 11.21 | 4.21e-4 | -3.38 | |
| 3 | 1.000 | 33.61 | 15.82 | 1.73e-5 | -4.76 | 13.68 | 7.63e-5 | -4.12 | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.997 | 31.9 | 17.86 | 4.21e-6 | -5.38 | 11.61 | 3.19e-4 | -3.5 | |
| 3 | 1.000 | 34.19 | 20.31 | 7.69e-7 | -6.11 | 14.75 | 3.64e-5 | -4.44 | |
| 35day-aerial | 1 | 1.000 | 31.45 | 16.99 | 7.66e-6 | -5.12 | 11.46 | 3.55e-4 | -3.45 |
| 2 | 0.998 | 29.96 | 17.24 | 6.48e-6 | -5.19 | 11.1 | 4.55e-4 | -3.34 | |
| 3 | 0.999 | 31.68 | 18.49 | 2.72e-6 | -5.57 | 12.3 | 1.98e-4 | -3.7 | |
| 42day-rosette | 1 | 1.000 | 32.64 | 19.1 | 1.77e-6 | -5.75 | 12 | 2.44e-4 | -3.61 |
| 2 | 0.999 | 29.79 | 15.41 | 2.30e-5 | -4.64 | 9.72 | 1.19e-3 | -2.93 | |
| 3 | 1.000 | 33.63 | 20.11 | 8.84e-7 | -6.05 | 14.54 | 4.19e-5 | -4.38 | |
| 42day-stem | 1 | 1.000 | 29.8 | 18.11 | 3.53e-6 | -5.45 | 12.83 | 1.40e-1 | -3.85 |
| 2 | 0.998 | 30.83 | 18.28 | 3.13e-1 | -5.54 | 12.73 | 1.47e-1 | -3.83 | |
| 3 | 0.999 | 32.05 | 18.66 | 2.50e-6 | -5.62 | 12.36 | 1.98e-1 | -3.72 | |
| 53day-rosette | 1 | 1.000 | 28.81 | 14.76 | 3.60e-5 | -4.44 | 8.77 | 2.29e-3 | -2.64 |
| 2 | 1.000 | 28.28 | 16 | 1.53e-5 | -4.82 | 10.28 | 8.03e-4 | -3.1 | |
| 3 | 1.000 | 28.2 | 15.31 | 2.45e-5 | -4.61 | 8.66 | 2.48e-3 | -2.61 | |
| 53day-stem | 1 | 1.000 | 34.12 | 20.7 | 5.88e-7 | -6.23 | 14.18 | 5.40e-5 | -4.27 |
| 2 | 1.000 | 29.72 | 14.94 | 3.17e-5 | -4.5 | 10.29 | 7.98e-4 | -3.1 | |
| 3 | 1.000 | 29.36 | 11.76 | 2.89e-4 | -3.54 | 8.8 | 2.24e-3 | -2.65 | |
| 53day-inflores | 1 | 0.998 | 32.41 | 19.02 | 1.88e-6 | -5.73 | 13.87 | 6.70e-5 | -4.17 |
| 2 | 1.000 | 29.88 | 15.25 | 2.56e-5 | -4.59 | 10.7 | 6.02e-4 | -3.22 | |
| 3 | 1.000 | 29.64 | 15.81 | 1.74e-5 | -4.76 | 11.22 | 4.20e-4 | -3.38 | |
| 53day-silique | 1 | 1.000 | 27.91 | 8.06 | 3.74e-3 | -2.43 | 4.11 | 5.78e-2 | -1.24 |
| 2 | 1.000 | 30.99 | 11.7 | 3.01e-4 | -3.52 | 7.99 | 3.92e-3 | -2.41 | |
| 3 | 1.000 | 32.29 | 13.32 | 9.77e-5 | -4.01 | 9.59 | 1.30e-3 | -2.89 | |
NGS expression data
Expression of miR399a transcript in NGS dataSequences
miRNA transcript
CAUUGUACCGUGGAAUAUGCUUCUUUCCAAGUUUAUAUAACAUCAAAUUUGCAAGUUACAAUUGUGAACAUGUGAGAUGGAUCAAAGGUGGAAAUGCAUUACAGGGUAAGAUCUCUAUUGGCAGGAAACCAUUACUUAGAUCUUUGCAUCUCUUUAUGCAUUGCUUUUAAUUAGUGAGUUAUCUGCCAAAGGAGAUUUGCCCUGUAAUUCUUCU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCAAAGGAGAUUUGCCCUG | miRNA | 184 | 204 | |
CATTGTACCGTGGAATATGCTTCTT | primer | 5p | 1 | 25 |
CCTGCCAATAGAGATCTTACCCTGTA | primer | 3p | 100 | 125 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR399a found in PubMed |