Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.19394 | 0.809049 | 5.26645 | 0.901238 | |
| 10day-seedling | 4.50732 | 0.558501 | 6.315478 | 0.384283 | |
| 14day-seedling | 4.29224 | 0.318138 | 5.46693 | 0.698427 | |
| 25day-aerial | 4.76402 | 0.571529 | 6.815199 | 0.818271 | |
| 35day-aerial | 4.90968 | 0.598433 | 6.736583 | 0.519027 | |
| 42day-rosette | 4.67842 | 0.348077 | 6.521135 | 0.262331 | 42day-stem | 4.12973 | 0.371534 | 5.87287 | 0.495544 |
| 53day-rosette | 5.26289 | 0.381716 | 7.105398 | 0.35562 | 53day-stem | 4.38365 | 0.80523 | 5.68968 | 0.428676 | 53day-inflores | 3.59331 | 0.118068 | 5.05239 | 0.172014 | 53day-silique | 5.19517 | 0.213694 | 6.337767 | 0.178701 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 28 | 4.15 | 5.65e-2 | -1.25 | 4.06 | 6.00e-2 | -1.22 |
| 2 | 1.000 | 37.06 | 9.08 | 1.85e-3 | -2.73 | 9.22 | 1.68e-3 | -2.77 | |
| 3 | 1.000 | 29.04 | 4.77 | 3.66e-2 | -1.44 | 4 | 6.24e-2 | -1.2 | |
| 10day-seedling | 1 | 1.000 | 19.21 | 6.35 | 1.22e-2 | -1.91 | 0.86 | 5.51e-1 | -0.26 |
| 2 | 1.000 | 21.68 | 8.43 | 2.90e-3 | -2.54 | 2.62 | 1.63e-1 | -0.79 | |
| 3 | 1.000 | 21.43 | 10.06 | 9.39e-4 | -3.03 | 3.34 | 9.85e-2 | -1.01 | |
| 14day-seedling | 1 | 0.999 | 22.74 | 9.17 | 1.73e-3 | -2.76 | 2.53 | 1.73e-1 | -0.76 |
| 2 | 0.988 | 28.06 | 9.95 | 1.01e-3 | -3 | 7.05 | 7.56e-3 | -2.12 | |
| 3 | 1.000 | 25.63 | 7.84 | 4.36e-3 | -2.36 | 5.7 | 1.92e-2 | -1.72 | |
| 25day-aerial | 1 | 0.999 | 18.41 | 5.56 | 2.12e-2 | -1.67 | -0.2 | 1.15e+0 | 0.06 |
| 2 | 0.999 | 23.35 | 9.31 | 1.58e-3 | -2.8 | 3.06 | 1.20e-1 | -0.92 | |
| 3 | 0.999 | 20.53 | 6.65 | 9.95e-3 | -2 | 1.09 | 4.70e-1 | -0.33 | |
| 35day-aerial | 1 | 0.999 | 19.35 | 4.89 | 3.37e-2 | -1.47 | -0.65 | 1.57e+0 | 0.19 |
| 2 | 0.998 | 21.58 | 8.86 | 2.15e-3 | -2.67 | 2.73 | 1.51e-1 | -0.82 | |
| 3 | 0.995 | 19.88 | 6.69 | 9.70e-3 | -2.01 | 0.5 | 7.07e-1 | -0.15 | |
| 42day-rosette | 1 | 1.000 | 22.06 | 8.52 | 2.72e-3 | -2.56 | 1.42 | 3.75e-1 | -0.43 |
| 2 | 0.999 | 22.61 | 8.23 | 3.33e-3 | -2.48 | 2.54 | 1.72e-1 | -0.76 | |
| 3 | 0.999 | 19.91 | 6.39 | 1.19e-2 | -1.92 | 0.82 | 5.66e-1 | -0.25 | |
| 42day-stem | 1 | 1.000 | 21.62 | 9.93 | 1.28e-1 | -2.99 | 4.62 | 4.80e-1 | -1.39 |
| 2 | 0.999 | 22.86 | 1.33 | 7.82e-1 | -3.17 | 4.77 | 3.66e-1 | -1.44 | |
| 3 | 0.999 | 21.54 | 8.15 | 3.51e-1 | -2.45 | 1.85 | 2.78e-1 | -0.56 | |
| 53day-rosette | 1 | 1.000 | 18.37 | 4.32 | 5.01e-2 | -1.3 | -1.67 | 3.18e+0 | 0.5 |
| 2 | 1.000 | 18.61 | 6.33 | 1.25e-2 | -1.9 | 0.61 | 6.55e-1 | -0.18 | |
| 3 | 1.000 | 19.56 | 6.67 | 9.85e-3 | -2.01 | - | - | N/D | |
| 53day-stem | 1 | 0.998 | 23.47 | 10.05 | 9.44e-4 | -3.03 | 3.53 | 8.66e-2 | -1.06 |
| 2 | 0.999 | 24.35 | 9.58 | 1.31e-3 | -2.88 | 4.92 | 3.30e-2 | -1.48 | |
| 3 | 1.000 | 23.29 | 5.69 | 1.94e-2 | -1.71 | 2.73 | 1.51e-1 | -0.82 | |
| 53day-inflores | 1 | 0.998 | 24.76 | 11.37 | 3.78e-4 | -3.42 | 6.22 | 1.35e-2 | -1.87 |
| 2 | 0.998 | 26.31 | 11.68 | 3.05e-4 | -3.52 | 7.12 | 7.17e-3 | -2.14 | |
| 3 | 0.998 | 24.49 | 10.66 | 6.19e-4 | -3.21 | 6.07 | 1.49e-2 | -1.83 | |
| 53day-silique | 1 | 1.000 | 26.53 | 6.68 | 9.75e-3 | -2.01 | 2.73 | 1.51e-1 | -0.82 |
| 2 | 1.000 | 24.55 | 5.26 | 2.61e-2 | -1.58 | 1.56 | 3.40e-1 | -0.47 | |
| 3 | 1.000 | 25.02 | 6.04 | 1.52e-2 | -1.82 | 2.31 | 2.01e-1 | -0.7 | |
NGS expression data
Expression of miR398c transcript in NGS dataSequences
miRNA transcript
GAAGGAUAUCGAAACUCAAACUGUAACAGUCCUUUUAUUACUGGUUUAGAAGAUAGAUAAAUAUUGUUAAGGUAGUGGAUCUCGACAGGGUUGAUAUGAGAACACACGAGCAAUCAACGGCUAUAACGACGCUACGUCAUUGUUACAGCUCUCGUUUCAUGUGUUCUCAGGUCACCCCUGCUGAGCUCUUUCUCUACCGUCCAUGUUUUAUCAACGCCGUGGCCCGUGGCUUCUAUUCAUUUACCAAAUGGU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGUGUUCUCAGGUCACCCCUG | miRNA | 160 | 180 | |
GAAGGATATCGAAACTCAAACTGTAACA | primer | 5p | 1 | 28 |
CGTCGTTATAGCCGTTGATTGC | primer | 3p | 110 | 131 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR398c found in PubMed |