Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
Read more
Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.05929 | 0.675043 | 5.18819 | 0.820314 | |
| 10day-seedling | 3.24201 | 0.215911 | 5.05017 | 0.0596663 | |
| 14day-seedling | 2.79107 | 0.185315 | 3.93754 | 0.758784 | |
| 25day-aerial | 2.82634 | 0.483346 | 4.73275 | 0.304583 | |
| 35day-aerial | 3.3806 | 0.294414 | 5.11465 | 0.696498 | |
| 42day-rosette | 3.34277 | 0.520547 | 5.16289 | 0.298923 | 42day-stem | 2.69161 | 0.106598 | 4.49269 | 0.356866 |
| 53day-rosette | 3.60914 | 0.28525 | 5.45164 | 0.218909 | 53day-stem | 3.21404 | 0.50074 | 4.62975 | 0.03818 | 53day-inflores | 2.68358 | 0.12976 | 4.11831 | 0.0288116 | 53day-silique | 3.7344 | 0.72393 | 4.89072 | 0.770455 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 31.89 | 8.03 | 3.82e-3 | -2.42 | 7.95 | 4.06e-3 | -2.39 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 29.13 | 4.86 | 3.44e-2 | -1.46 | 4.09 | 5.86e-2 | -1.23 | |
| 10day-seedling | 1 | 1.000 | 24.63 | 11.77 | 2.87e-4 | -3.54 | 6.27 | 1.29e-2 | -1.89 |
| 2 | 1.000 | 25.73 | 12.48 | 1.75e-4 | -3.76 | 6.67 | 9.83e-3 | -2.01 | |
| 3 | 1.000 | 24.57 | 13.2 | 1.06e-4 | -3.97 | 6.49 | 1.11e-2 | -1.95 | |
| 14day-seedling | 1 | 0.998 | 27.77 | 14.2 | 5.32e-5 | -4.27 | 7.55 | 5.32e-3 | -2.27 |
| 2 | 0.000 | 31.4 | 13.29 | 1.00e-4 | -4 | 10.38 | 7.49e-4 | -3.13 | |
| 3 | 0.999 | 32.25 | 14.46 | 4.44e-5 | -4.35 | 12.32 | 1.96e-4 | -3.71 | |
| 25day-aerial | 1 | 1.000 | 26.31 | 13.45 | 8.95e-5 | -4.05 | 7.69 | 4.85e-3 | -2.31 |
| 2 | 0.998 | 28.74 | 14.7 | 3.76e-5 | -4.42 | 8.46 | 2.85e-3 | -2.55 | |
| 3 | 0.999 | 25.89 | 12.01 | 2.42e-4 | -3.62 | 6.45 | 1.14e-2 | -1.94 | |
| 35day-aerial | 1 | 1.000 | 24.61 | 10.15 | 8.78e-4 | -3.06 | 4.62 | 4.07e-2 | -1.39 |
| 2 | 0.998 | 23.87 | 11.14 | 4.42e-4 | -3.35 | 5.01 | 3.10e-2 | -1.51 | |
| 3 | 0.999 | 26.25 | 13.06 | 1.17e-4 | -3.93 | 6.87 | 8.55e-3 | -2.07 | |
| 42day-rosette | 1 | 0.999 | 27.64 | 14.1 | 5.68e-5 | -4.25 | 7 | 7.82e-3 | -2.11 |
| 2 | 0.998 | 25.91 | 11.53 | 3.39e-4 | -3.47 | 5.83 | 1.75e-2 | -1.76 | |
| 3 | 0.999 | 24.34 | 10.82 | 5.54e-4 | -3.26 | 5.25 | 2.63e-2 | -1.58 | |
| 42day-stem | 1 | 0.999 | 26.4 | 14.71 | 3.72e-1 | -4.43 | 9.44 | 1.48e-1 | -2.84 |
| 2 | 1.000 | 26.59 | 14.42 | 5.93e-1 | -4.23 | 8.49 | 2.77e-1 | -2.56 | |
| 3 | 0.999 | 26.76 | 13.38 | 9.40e-1 | -4.27 | 7.71 | 7.44e-1 | -2.13 | |
| 53day-rosette | 1 | 1.000 | 25.94 | 11.9 | 2.62e-4 | -3.58 | 5.91 | 1.67e-2 | -1.78 |
| 2 | 1.000 | 22.46 | 10.17 | 8.65e-4 | -3.06 | 4.46 | 4.55e-2 | -1.34 | |
| 3 | 1.000 | 24.61 | 11.72 | 2.96e-4 | -3.53 | 5.07 | 2.99e-2 | -1.52 | |
| 53day-stem | 1 | 1.000 | 27.65 | 14.23 | 5.21e-5 | -4.28 | 7.71 | 4.78e-3 | -2.32 |
| 2 | 1.000 | 27.37 | 12.6 | 1.61e-4 | -3.79 | 7.95 | 4.06e-3 | -2.39 | |
| 3 | 1.000 | 28.51 | 10.9 | 5.22e-4 | -3.28 | 7.95 | 4.05e-3 | -2.39 | |
| 53day-inflores | 1 | 1.000 | 28.23 | 14.84 | 3.42e-5 | -4.47 | 9.68 | 1.22e-3 | -2.91 |
| 2 | 1.000 | 28.71 | 14.08 | 5.76e-5 | -4.24 | 9.53 | 1.35e-3 | -2.87 | |
| 3 | 0.999 | 27.93 | 14.1 | 5.71e-5 | -4.24 | 9.51 | 1.37e-3 | -2.86 | |
| 53day-silique | 1 | 1.000 | 28.99 | 9.15 | 1.76e-3 | -2.75 | 5.2 | 2.73e-2 | -1.56 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 31.52 | 12.55 | 1.67e-4 | -3.78 | 8.82 | 2.22e-3 | -2.65 | |
NGS expression data
Expression of miR398a transcript in NGS dataSequences
miRNA transcript
GCAGUGAAAGCAAAGUAUGGAGAAGAAGAGAAGAACAACAGGAGGUGAAAUUUCAAAGGAGUGGCAUGUGAACACAUAUCCUAUGGUUUCUUCAAAUUUCCAUUGAAACCAUUGAGUUUUGUGUUCUCAGGUCACCCCUUUGAAUCUCCCCUGUUCCA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGUGUUCUCAGGUCACCCCUU | miRNA | 120 | 140 | |
GCAGTGAAAGCAAAGTATGGAG | primer | 5p | 1 | 22 |
TGGAACAGGGGAGATTCAAAGGG | primer | 3p | 136 | 158 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR398a found in PubMed |