Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.49726 | 0.118638 | 3.56978 | 0.258343 | |
| 10day-seedling | 1.5515 | 0.471919 | 3.38879 | 0.212506 | |
| 14day-seedling | 2.08183 | 0 | 4.08178 | 0 | |
| 25day-aerial | 0.80518 | 0 | 2.54314 | 0 | |
| 35day-aerial | -0.51782 | 0 | 1.32833 | 0 | |
| 42day-rosette | 1.32619 | 0 | 3.00217 | 0 | 42day-stem | 0.16884 | 0.758258 | 1.8033 | 0.707407 |
| 53day-rosette | 1.08575 | 0.253953 | 2.94807 | 0.053651 | 53day-stem | 0.83849 | 1.4198 | 2.23318 | 0.948966 | 53day-inflores | 1.92022 | 0.131572 | 3.26699 | 0.113382 | 53day-silique | 3.09399 | 0.573762 | 4.23659 | 0.609214 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 35.73 | 11.87 | 2.67e-4 | -3.57 | 11.78 | 2.84e-4 | -3.55 |
| 2 | 1.000 | 39.84 | 11.86 | 2.69e-4 | -3.57 | 11.99 | 2.46e-4 | -3.61 | |
| 3 | 1.000 | 35.45 | 11.18 | 4.31e-4 | -3.37 | 10.41 | 7.34e-4 | -3.13 | |
| 10day-seedling | 1 | 1.000 | 29.85 | 16.99 | 7.68e-6 | -5.11 | 11.5 | 3.46e-4 | -3.46 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 30.58 | 19.21 | 1.65e-6 | -5.78 | 12.5 | 1.73e-4 | -3.76 | |
| 14day-seedling | 1 | 1.000 | 29.91 | 16.34 | 1.21e-5 | -4.92 | 9.69 | 1.21e-3 | -2.92 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | 0.998 | 33.42 | 20.56 | 6.45e-7 | -6.19 | 14.81 | 3.49e-5 | -4.46 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.984 | 37.7 | 24.97 | 3.04e-8 | -7.52 | 18.84 | 2.13e-6 | -5.67 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.999 | 32.37 | 18.85 | 2.12e-6 | -5.67 | 13.28 | 1.01e-4 | -4 | |
| 42day-stem | 1 | 0.999 | 32.6 | 2.91 | 5.74e-7 | -6.29 | 15.61 | 2.11e-1 | -4.7 |
| 2 | 0.995 | 37.02 | 24.47 | 4.29e-1 | -7.37 | 18.92 | 2.95e-1 | -5.7 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 31.34 | 19.05 | 1.84e-6 | -5.73 | 13.33 | 9.68e-5 | -4.01 | |
| 3 | 1.000 | 33.13 | 20.24 | 8.06e-7 | -6.09 | 13.59 | 8.13e-5 | -4.09 | |
| 53day-stem | 1 | 1.000 | 37.57 | 24.15 | 5.36e-8 | -7.27 | 17.63 | 4.92e-6 | -5.31 |
| 2 | 1.000 | 36.85 | 22.07 | 2.27e-7 | -6.64 | 17.42 | 5.70e-6 | -5.24 | |
| 3 | 1.000 | 32.76 | 15.16 | 2.73e-5 | -4.56 | 12.2 | 2.12e-4 | -3.67 | |
| 53day-inflores | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.999 | 31.81 | 17.18 | 6.72e-6 | -5.17 | 12.63 | 1.58e-4 | -3.8 | |
| 3 | 1.000 | 30.39 | 16.57 | 1.03e-5 | -4.99 | 11.98 | 2.48e-4 | -3.61 | |
| 53day-silique | 1 | 1.000 | 30.82 | 10.97 | 4.99e-4 | -3.3 | 7.02 | 7.71e-3 | -2.11 |
| 2 | 1.000 | 32.49 | 13.2 | 1.07e-4 | -3.97 | 9.49 | 1.39e-3 | -2.86 | |
| 3 | 1.000 | 33.73 | 14.76 | 3.60e-5 | -4.44 | 11.03 | 4.78e-4 | -3.32 | |
NGS expression data
Expression of miR397b transcript in NGS dataSequences
miRNA transcript
CAAAAAGAAUCUACAGCCCCCAAAAAAUAAGAUUUAGUACAAUUCCUUAAUCGUACACAGUAGUACAAGCAAGCAUUGUACUGCUUUCAAACCAAAGACACGACCAAAUGUCUUAUAAAUGAUAUUUGUGUUUAUCUCCAUGGUAAUAGAAAUGAGCAAGGAGAUAUUUUUUUCCCCUGGGUUUGAAUGAACAUCAUUGAGUGCAUCGUUGAUGUAAUUUUACUUAUUUUAUUCCAUUGUUGAAUUAAUUAAAGAAGUAUAUAUCAGCGUUGCAUUCAAUUAUGUUUUUCUA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCAUUGAGUGCAUCGUUGAUG | miRNA | 194 | 214 | |
CAAAAAGAATCTACAGCCCCCAAA | primer | 5p | 1 | 24 |
GTTCATTCAAACCCAGGGGAAA | primer | 3p | 171 | 192 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR397b found in PubMed |