Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.83767 | 0 | 4.86385 | 0 | |
| 10day-seedling | 3.77497 | 0.656086 | 5.58312 | 0.50947 | |
| 14day-seedling | 3.30933 | 0.261131 | 4.48122 | 0.480782 | |
| 25day-aerial | 3.38278 | 0.571161 | 5.24383 | 0.350863 | |
| 35day-aerial | 3.26537 | 0.511562 | 5.33014 | 0.444038 | |
| 42day-rosette | 3.1987 | 0.434829 | 5.06079 | 0.3319 | 42day-stem | 3.36513 | 0.335227 | 5.08783 | 0.484382 |
| 53day-rosette | 3.56497 | 0.434592 | 5.40747 | 0.359423 | 53day-stem | 4.08697 | 0.75899 | 5.50425 | 0.222295 | 53day-inflores | 3.38547 | 0.257625 | 4.84836 | 0.208601 | 53day-silique | 4.82007 | 0.219032 | 5.96267 | 0.206799 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 31.04 | 7.18 | 6.88e-3 | -2.16 | 7.1 | 7.31e-3 | -2.14 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 10day-seedling | 1 | 1.000 | 21.11 | 8.25 | 3.29e-3 | -2.48 | 2.75 | 1.48e-1 | -0.83 |
| 2 | 1.000 | 24.76 | 11.51 | 3.42e-4 | -3.47 | 5.7 | 1.92e-2 | -1.72 | |
| 3 | 1.000 | 23.75 | 12.38 | 1.88e-4 | -3.73 | 5.67 | 1.97e-2 | -1.71 | |
| 14day-seedling | 1 | 1.000 | 26.74 | 13.17 | 1.09e-4 | -3.96 | 6.52 | 1.09e-2 | -1.96 |
| 2 | 0.000 | 30.28 | 12.17 | 2.16e-4 | -3.66 | 9.27 | 1.62e-3 | -2.79 | |
| 3 | 1.000 | 29.23 | 11.44 | 3.60e-4 | -3.44 | 9.3 | 1.59e-3 | -2.8 | |
| 25day-aerial | 1 | 1.000 | 23.51 | 10.65 | 6.23e-4 | -3.21 | 4.89 | 3.38e-2 | -1.47 |
| 2 | 0.998 | 27.42 | 13.38 | 9.39e-5 | -4.03 | 7.14 | 7.11e-3 | -2.15 | |
| 3 | 0.999 | 24.92 | 11.04 | 4.75e-4 | -3.32 | 5.48 | 2.25e-2 | -1.65 | |
| 35day-aerial | 1 | 0.998 | 24.67 | 10.21 | 8.43e-4 | -3.07 | 4.68 | 3.91e-2 | -1.41 |
| 2 | 0.999 | 26.09 | 13.37 | 9.46e-5 | -4.02 | 7.24 | 6.64e-3 | -2.18 | |
| 3 | 0.999 | 23.89 | 10.69 | 6.04e-4 | -3.22 | 4.5 | 4.40e-2 | -1.36 | |
| 42day-rosette | 1 | 0.997 | 26.88 | 13.34 | 9.63e-5 | -4.02 | 6.24 | 1.33e-2 | -1.88 |
| 2 | 0.998 | 27.41 | 13.03 | 1.19e-4 | -3.92 | 7.34 | 6.17e-3 | -2.21 | |
| 3 | 1.000 | 24.55 | 11.02 | 4.80e-4 | -3.32 | 5.46 | 2.28e-2 | -1.64 | |
| 42day-stem | 1 | 1.000 | 24.93 | 13.24 | 1.32e-1 | -3.99 | 7.93 | 4.95e-1 | -2.39 |
| 2 | 1.000 | 24.5 | 11.96 | 2.51e-1 | -3.6 | 6.49 | 1.18e-1 | -1.93 | |
| 3 | 0.999 | 24.41 | 11.22 | 4.88e-1 | -3.32 | 4.72 | 3.87e-1 | -1.42 | |
| 53day-rosette | 1 | 1.000 | 23.96 | 9.91 | 1.04e-3 | -2.98 | 3.92 | 6.58e-2 | -1.18 |
| 2 | 1.000 | 23.81 | 11.52 | 3.39e-4 | -3.47 | 5.81 | 1.78e-2 | -1.75 | |
| 3 | 1.000 | 25.69 | 12.79 | 1.41e-4 | -3.85 | 6.14 | 1.42e-2 | -1.85 | |
| 53day-stem | 1 | 0.999 | 25.65 | 12.22 | 2.09e-4 | -3.68 | 5.7 | 1.92e-2 | -1.72 |
| 2 | 1.000 | 24.4 | 9.63 | 1.27e-3 | -2.9 | 4.97 | 3.18e-2 | -1.5 | |
| 3 | 1.000 | 24.79 | 7.18 | 6.89e-3 | -2.16 | 4.23 | 5.34e-2 | -1.27 | |
| 53day-inflores | 1 | 0.992 | 26.32 | 12.92 | 1.29e-4 | -3.89 | 7.77 | 4.58e-3 | -2.34 |
| 2 | 1.000 | 26.45 | 11.83 | 2.76e-4 | -3.56 | 7.27 | 6.48e-3 | -2.19 | |
| 3 | 0.999 | 24.82 | 10.99 | 4.91e-4 | -3.31 | 6.4 | 1.18e-2 | -1.93 | |
| 53day-silique | 1 | 1.000 | 27.33 | 7.48 | 5.58e-3 | -2.25 | 3.53 | 8.63e-2 | -1.06 |
| 2 | 1.000 | 25.72 | 6.42 | 1.17e-2 | -1.93 | 2.72 | 1.52e-1 | -0.82 | |
| 3 | 1.000 | 26.79 | 7.82 | 4.44e-3 | -2.35 | 4.08 | 5.89e-2 | -1.23 | |
NGS expression data
Expression of miR397a transcript in NGS dataSequences
miRNA transcript
UGAAUGAACAUCAUUGAGUGCAGCGUUGAUGUAAUUUCGUUUUGUUUUUCAUUGUUGAAUGGAUUAAAAGAAUUUAUACCAGCGUUGCGCUCAAUUAUGUUUUUCUAAUUUUCAGGAACACUUAAACAGGUAUGCGGAUCGU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCAUUGAGUGCAGCGUUGAUG | miRNA | 11 | 31 | |
GCGTTGATGTAATTTCGTTTTGTTT | primer | 5p | 23 | 47 |
ACGATCCGCATACCTGTTTAAGTG | primer | 3p | 119 | 142 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR397a found in PubMed |