Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.14139 | 0.867103 | 4.2703 | 1.01237 | |
| 10day-seedling | 2.9713 | 0.76842 | 4.77946 | 0.588492 | |
| 14day-seedling | 3.63501 | 0.439601 | 4.83013 | 0.283382 | |
| 25day-aerial | 3.39533 | 0.274337 | 5.15933 | 0.308392 | |
| 35day-aerial | 3.17833 | 0.561082 | 4.97219 | 0.477361 | |
| 42day-rosette | 3.14325 | 0.755016 | 4.98477 | 0.657782 | 42day-stem | 2.57085 | 0.500992 | 4.27684 | 0.521968 |
| 53day-rosette | 3.73196 | 0.442079 | 5.57446 | 0.310133 | 53day-stem | 2.77131 | 0.902381 | 4.18896 | 0.990081 | 53day-inflores | 2.53102 | 0.263181 | 3.98574 | 0.196515 | 53day-silique | 2.97292 | 1.10786 | 4.11551 | 1.14512 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 35.39 | 11.53 | 3.37e-4 | -3.47 | 11.45 | 3.58e-4 | -3.45 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 31.73 | 7.46 | 5.68e-3 | -2.25 | 6.69 | 9.69e-3 | -2.01 | |
| 10day-seedling | 1 | 1.000 | 23.66 | 10.8 | 5.60e-4 | -3.25 | 5.31 | 2.52e-2 | -1.6 |
| 2 | 1.000 | 26.69 | 13.44 | 8.99e-5 | -4.05 | 7.63 | 5.06e-3 | -2.3 | |
| 3 | 1.000 | 27.28 | 15.91 | 1.63e-5 | -4.79 | 9.19 | 1.71e-3 | -2.77 | |
| 14day-seedling | 1 | 1.000 | 26.34 | 12.77 | 1.44e-4 | -3.84 | 6.12 | 1.44e-2 | -1.84 |
| 2 | 0.999 | 28.77 | 10.65 | 6.20e-4 | -3.21 | 7.75 | 4.65e-3 | -2.33 | |
| 3 | 1.000 | 27.68 | 9.89 | 1.05e-3 | -2.98 | 7.75 | 4.64e-3 | -2.33 | |
| 25day-aerial | 1 | 0.999 | 25.88 | 13.02 | 1.20e-4 | -3.92 | 7.26 | 6.52e-3 | -2.19 |
| 2 | 0.998 | 25.57 | 11.53 | 3.38e-4 | -3.47 | 5.29 | 2.56e-2 | -1.59 | |
| 3 | 1.000 | 25.22 | 11.34 | 3.84e-4 | -3.42 | 5.78 | 1.82e-2 | -1.74 | |
| 35day-aerial | 1 | 0.999 | 25.25 | 10.8 | 5.61e-4 | -3.25 | 5.26 | 2.61e-2 | -1.58 |
| 2 | 0.999 | 27.27 | 14.55 | 4.18e-5 | -4.38 | 8.41 | 2.93e-3 | -2.53 | |
| 3 | 0.992 | 25.91 | 12.72 | 1.48e-4 | -3.83 | 6.53 | 1.08e-2 | -1.97 | |
| 42day-rosette | 1 | 0.999 | 27.67 | 14.13 | 5.59e-5 | -4.25 | 7.02 | 7.70e-3 | -2.11 |
| 2 | 1.000 | 28.77 | 14.39 | 4.66e-5 | -4.33 | 8.7 | 2.41e-3 | -2.62 | |
| 3 | 1.000 | 23.44 | 9.92 | 1.03e-3 | -2.99 | 4.35 | 4.90e-2 | -1.31 | |
| 42day-stem | 1 | 0.997 | 26.61 | 14.92 | 3.23e-1 | -4.49 | 9.7 | 1.28e-1 | -2.89 |
| 2 | 0.999 | 28.52 | 15.98 | 1.55e-1 | -4.9 | 1.43 | 7.26e-1 | -3.14 | |
| 3 | 1.000 | 26.34 | 12.96 | 1.26e-1 | -3.9 | 6.65 | 9.97e-1 | -2.14 | |
| 53day-rosette | 1 | 1.000 | 23.95 | 9.9 | 1.05e-3 | -2.98 | 3.91 | 6.65e-2 | -1.18 |
| 2 | 1.000 | 22.41 | 10.12 | 8.97e-4 | -3.05 | 4.41 | 4.71e-2 | -1.33 | |
| 3 | 1.000 | 25.44 | 12.55 | 1.67e-4 | -3.78 | 5.89 | 1.69e-2 | -1.77 | |
| 53day-stem | 1 | 1.000 | 26.22 | 12.8 | 1.41e-4 | -3.85 | 6.28 | 1.29e-2 | -1.89 |
| 2 | 0.999 | 32.24 | 17.47 | 5.52e-6 | -5.26 | 12.82 | 1.39e-4 | -3.86 | |
| 3 | 1.000 | 29.48 | 11.88 | 2.66e-4 | -3.58 | 8.92 | 2.06e-3 | -2.69 | |
| 53day-inflores | 1 | 0.999 | 28.88 | 15.49 | 2.18e-5 | -4.66 | 10.33 | 7.75e-4 | -3.11 |
| 2 | 0.998 | 29.6 | 14.97 | 3.11e-5 | -4.51 | 10.42 | 7.31e-4 | -3.14 | |
| 3 | 1.000 | 27.68 | 13.85 | 6.77e-5 | -4.17 | 9.26 | 1.63e-3 | -2.79 | |
| 53day-silique | 1 | 1.000 | 29.53 | 9.68 | 1.22e-3 | -2.91 | 5.73 | 1.88e-2 | -1.73 |
| 2 | 1.000 | 36.33 | 17.04 | 7.41e-6 | -5.13 | 13.34 | 9.66e-5 | -4.02 | |
| 3 | 1.000 | 32.38 | 13.41 | 9.20e-5 | -4.04 | 9.68 | 1.22e-3 | -2.91 | |
NGS expression data
Expression of miR395b transcript in NGS dataSequences
miRNA transcript
AUGUCCCCAUGAGUUCCCUUUAACGCUUCAUUGUUAAAUACUCAAAGCCACAUUGGUUUGUAUACAACACUGAAGUGUUUGGGGGGACUCUUGGUGUCAUUCUGGCGUAGUGACCACCAUUGCAGCC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CUGAAGUGUUUGGGGGGACUC | miRNA | 70 | 90 | |
TCCCCATGAGTTCCCTTTAACG | primer | 5p | 4 | 25 |
GGCTGCAATGGTGGTCACTAC | primer | 3p | 107 | 127 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR395b found in PubMed |