Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.59312 | 0.411007 | 4.66564 | 0.276244 | |
| 10day-seedling | 2.57785 | 0.294964 | 4.386 | 0.118509 | |
| 14day-seedling | 2.43554 | 0.27048 | 3.59638 | 1.26762 | |
| 25day-aerial | 2.50021 | 0.954459 | 4.31235 | 1.02687 | |
| 35day-aerial | 2.56474 | 0.787697 | 4.35672 | 0.744719 | |
| 42day-rosette | 2.47538 | 1.16733 | 4.1638 | 1.47087 | 42day-stem | 1.39559 | 1.14126 | 3.17066 | 1.23739 |
| 53day-rosette | 3.35618 | 0.642092 | 5.19869 | 0.564153 | 53day-stem | 1.77387 | 1.17821 | 3.19363 | 1.1555 | 53day-inflores | 1.22344 | 0.693116 | 2.54748 | 0.49947 | 53day-silique | 3.32381 | 0.812476 | 4.46641 | 0.852984 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 31.04 | 7.18 | 6.89e-3 | -2.16 | 7.09 | 7.32e-3 | -2.14 |
| 2 | 1.000 | 35.21 | 7.23 | 6.64e-3 | -2.18 | 7.37 | 6.05e-3 | -2.22 | |
| 3 | 1.000 | 33.84 | 9.57 | 1.31e-3 | -2.88 | 8.8 | 2.24e-3 | -2.65 | |
| 10day-seedling | 1 | 1.000 | 26.59 | 13.73 | 7.36e-5 | -4.13 | 8.24 | 3.32e-3 | -2.48 |
| 2 | 1.000 | 27.9 | 14.65 | 3.88e-5 | -4.41 | 8.84 | 2.18e-3 | -2.66 | |
| 3 | 1.000 | 27.06 | 15.69 | 1.89e-5 | -4.72 | 8.98 | 1.99e-3 | -2.7 | |
| 14day-seedling | 1 | 0.999 | 27.7 | 14.13 | 5.59e-5 | -4.25 | 7.48 | 5.60e-3 | -2.25 |
| 2 | 1.000 | 31.63 | 13.52 | 8.49e-5 | -4.07 | 10.62 | 6.36e-4 | -3.2 | |
| 3 | 0.990 | 33.47 | 15.68 | 1.91e-5 | -4.72 | 13.54 | 8.39e-5 | -4.08 | |
| 25day-aerial | 1 | 0.998 | 31.38 | 18.52 | 2.65e-6 | -5.58 | 12.77 | 1.44e-4 | -3.84 |
| 2 | 0.999 | 26.53 | 12.48 | 1.75e-4 | -3.76 | 6.24 | 1.32e-2 | -1.88 | |
| 3 | 1.000 | 27.22 | 13.34 | 9.63e-5 | -4.02 | 7.78 | 4.55e-3 | -2.34 | |
| 35day-aerial | 1 | 0.994 | 27.62 | 13.16 | 1.09e-4 | -3.96 | 7.62 | 5.07e-3 | -2.29 |
| 2 | 0.999 | 30.48 | 17.75 | 4.52e-6 | -5.34 | 11.62 | 3.17e-4 | -3.5 | |
| 3 | 0.998 | 26.48 | 13.28 | 1.00e-4 | -4 | 7.1 | 7.30e-3 | -2.14 | |
| 42day-rosette | 1 | 0.996 | 28.03 | 14.49 | 4.35e-5 | -4.36 | 7.38 | 5.99e-3 | -2.22 |
| 2 | 1.000 | 33.53 | 19.15 | 1.72e-6 | -5.76 | 13.46 | 8.88e-5 | -4.05 | |
| 3 | 1.000 | 24.97 | 11.45 | 3.57e-4 | -3.45 | 5.88 | 1.69e-2 | -1.77 | |
| 42day-stem | 1 | 0.998 | 28.36 | 16.67 | 9.59e-6 | -5.18 | 11.36 | 3.86e-1 | -3.42 |
| 2 | 0.999 | 35.43 | 22.89 | 1.29e-1 | -6.9 | 17.34 | 6.35e-1 | -5.22 | |
| 3 | 1.000 | 29.01 | 15.62 | 1.98e-1 | -4.73 | 9.32 | 1.57e-1 | -2.85 | |
| 53day-rosette | 1 | 1.000 | 23.91 | 9.86 | 1.08e-3 | -2.97 | 3.87 | 6.83e-2 | -1.17 |
| 2 | 1.000 | 24.63 | 12.35 | 1.92e-4 | -3.72 | 6.63 | 1.01e-2 | -2 | |
| 3 | 1.000 | 27 | 14.11 | 5.67e-5 | -4.25 | 7.45 | 5.72e-3 | -2.24 | |
| 53day-stem | 1 | 1.000 | 29.75 | 16.33 | 1.22e-5 | -4.91 | 9.81 | 1.12e-3 | -2.95 |
| 2 | 0.999 | 36.38 | 21.6 | 3.14e-7 | -6.5 | 16.95 | 7.90e-6 | -5.1 | |
| 3 | 0.999 | 31.67 | 14.07 | 5.82e-5 | -4.23 | 11.11 | 4.51e-4 | -3.35 | |
| 53day-inflores | 1 | 0.998 | 35.01 | 21.62 | 3.11e-7 | -6.51 | 16.46 | 1.11e-5 | -4.96 |
| 2 | 1.000 | 32.33 | 17.7 | 4.69e-6 | -5.33 | 13.15 | 1.10e-4 | -3.96 | |
| 3 | 1.000 | 31.85 | 18.03 | 3.75e-6 | -5.43 | 13.44 | 9.03e-5 | -4.04 | |
| 53day-silique | 1 | 1.000 | 28.98 | 9.14 | 1.77e-3 | -2.75 | 5.19 | 2.74e-2 | -1.56 |
| 2 | 1.000 | 33.49 | 14.19 | 5.33e-5 | -4.27 | 10.49 | 6.95e-4 | -3.16 | |
| 3 | 1.000 | 32.27 | 13.3 | 9.90e-5 | -4 | 9.57 | 1.31e-3 | -2.88 | |
NGS expression data
Expression of miR395a transcript in NGS dataSequences
miRNA transcript
UCUUCUCAAAACUUACAUCAACCUCUUGUACUCGUAUAGUCGUAUUCCACUCAUAGUACAUGAAAUACUCAAGAAACAAUAUUCUUGGUUGGAUGUCUCCUAGAGUUCCUCUGAGCACUUCAUUGGGGAUACAAUUUUUCUAAAUGAUUAUCCACUGAAGUGUUUGGGGGAACUCCCGGACCCAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CUGAAGUGUUUGGGGGAACUC | miRNA | 155 | 175 | |
TCTTCTCAAAACTTACATCAACCTCTTG | primer | 5p | 1 | 28 |
GTATCCCCAATGAAGTGCTCAGA | primer | 3p | 110 | 132 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR395a found in PubMed |