Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.5367 | 0.182289 | 3.60921 | 0.063022 | |
| 10day-seedling | 3.54828 | 0.399005 | 5.35644 | 0.268055 | |
| 14day-seedling | 4.41933 | 1.38514 | 6.013453 | 2.53181 | |
| 25day-aerial | 3.10124 | 0.0160704 | 4.80994 | 0.0484505 | |
| 35day-aerial | 3.1249 | 0.4146 | 4.91631 | 0.315064 | |
| 42day-rosette | 2.92445 | 0.356478 | 4.7696 | 0.614067 | 42day-stem | 3.57563 | 0.143037 | 5.29891 | 0.203248 |
| 53day-rosette | 3.27984 | 0.633139 | 5.12234 | 0.575749 | 53day-stem | 4.18003 | 0.790199 | 5.59778 | 0.29246 | 53day-inflores | 3.90104 | 0.145307 | 5.33595 | 0.156676 | 53day-silique | 3.84585 | 0.418453 | 4.98845 | 0.458205 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 35.37 | 11.51 | 3.42e-4 | -3.47 | 11.43 | 3.63e-4 | -3.44 |
| 2 | 1.000 | 38.87 | 10.89 | 5.25e-4 | -3.28 | 11.03 | 4.79e-4 | -3.32 | |
| 3 | 1.000 | 36.38 | 12.11 | 2.27e-4 | -3.64 | 11.34 | 3.87e-4 | -3.41 | |
| 10day-seedling | 1 | 1.000 | 22.82 | 9.96 | 1.01e-3 | -3 | 4.46 | 4.53e-2 | -1.34 |
| 2 | 1.000 | 25.24 | 11.99 | 2.45e-4 | -3.61 | 6.18 | 1.38e-2 | -1.86 | |
| 3 | 1.000 | 23.82 | 12.45 | 1.79e-4 | -3.75 | 5.73 | 1.88e-2 | -1.73 | |
| 14day-seedling | 1 | 0.621 | 13.9 | 0.33 | 7.98e-1 | -0.1 | -6.32 | 7.98e+1 | 1.9 |
| 2 | 0.999 | 28.21 | 10.1 | 9.12e-4 | -3.04 | 7.19 | 6.83e-3 | -2.17 | |
| 3 | 0.998 | 28.9 | 11.11 | 4.52e-4 | -3.35 | 8.97 | 1.99e-3 | -2.7 | |
| 25day-aerial | 1 | 0.998 | 25.76 | 12.9 | 1.31e-4 | -3.88 | 7.14 | 7.08e-3 | -2.15 |
| 2 | 0.999 | 26.99 | 12.94 | 1.27e-4 | -3.9 | 6.7 | 9.61e-3 | -2.02 | |
| 3 | 1.000 | 26.89 | 13.01 | 1.22e-4 | -3.92 | 7.44 | 5.75e-3 | -2.24 | |
| 35day-aerial | 1 | 0.999 | 25.81 | 11.35 | 3.83e-4 | -3.42 | 5.81 | 1.78e-2 | -1.75 |
| 2 | 1.000 | 26.76 | 14.03 | 5.96e-5 | -4.22 | 7.9 | 4.19e-3 | -2.38 | |
| 3 | 0.999 | 26.43 | 13.23 | 1.04e-4 | -3.98 | 7.05 | 7.56e-3 | -2.12 | |
| 42day-rosette | 1 | 0.995 | 25.7 | 12.16 | 2.18e-4 | -3.66 | 5.06 | 3.01e-2 | -1.52 |
| 2 | 0.999 | 28.77 | 14.39 | 4.65e-5 | -4.33 | 8.7 | 2.40e-3 | -2.62 | |
| 3 | 1.000 | 27.56 | 14.04 | 5.95e-5 | -4.23 | 8.47 | 2.82e-3 | -2.55 | |
| 42day-stem | 1 | 0.999 | 23.43 | 11.74 | 2.92e-1 | -3.53 | 6.43 | 1.16e-1 | -1.94 |
| 2 | 0.995 | 23.37 | 1.83 | 5.49e-1 | -3.26 | 5.28 | 2.57e-1 | -1.59 | |
| 3 | 0.998 | 24.93 | 11.55 | 3.34e-1 | -3.48 | 5.24 | 2.64e-1 | -1.58 | |
| 53day-rosette | 1 | 1.000 | 24.07 | 10.02 | 9.62e-4 | -3.02 | 4.03 | 6.11e-2 | -1.21 |
| 2 | 1.000 | 25.24 | 12.95 | 1.26e-4 | -3.9 | 7.24 | 6.62e-3 | -2.18 | |
| 3 | 1.000 | 26.99 | 14.1 | 5.70e-5 | -4.24 | 7.44 | 5.75e-3 | -2.24 | |
| 53day-stem | 1 | 1.000 | 25.71 | 12.29 | 2.00e-4 | -3.7 | 5.77 | 1.84e-2 | -1.74 |
| 2 | 1.000 | 23.39 | 8.61 | 2.55e-3 | -2.59 | 3.96 | 6.42e-2 | -1.19 | |
| 3 | 0.999 | 24.8 | 7.2 | 6.81e-3 | -2.17 | 4.24 | 5.28e-2 | -1.28 | |
| 53day-inflores | 1 | 0.999 | 23.84 | 10.45 | 7.15e-4 | -3.15 | 5.3 | 2.54e-2 | -1.59 |
| 2 | 0.998 | 25.31 | 10.68 | 6.10e-4 | -3.21 | 6.12 | 1.43e-2 | -1.84 | |
| 3 | 1.000 | 23.58 | 9.75 | 1.16e-3 | -2.94 | 5.16 | 2.79e-2 | -1.55 | |
| 53day-silique | 1 | 1.000 | 28.73 | 8.88 | 2.12e-3 | -2.67 | 4.93 | 3.28e-2 | -1.48 |
| 2 | 1.000 | 30.41 | 11.12 | 4.49e-4 | -3.35 | 7.42 | 5.85e-3 | -2.23 | |
| 3 | 1.000 | 30.4 | 11.43 | 3.63e-4 | -3.44 | 7.7 | 4.82e-3 | -2.32 | |
Nothern hybridization
NGS expression data
Expression of miR319c transcript in NGS dataSequences
miRNA transcript
UAGAUAUAGAAGGAGAUUCUUUCAGUCCAGUCAUGGAUAGAAAAAGAAGAGGGUAGAAAUAUCUGCCGACUCAUCCAUCCAAACACUCGUGGUAGAGAAACGAUAAAUUUAAACCGCAGUGACUGUGUGAAUGAUGCGGGAGAUAUUUUUGAUCCUUCUUUAUCUGUGUUUGGACUGAAGGGAGCUCCUUCUUUUUCUA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UUGGACUGAAGGGAGCUCCUU | miRNA | 170 | 190 | |
TCATGGATAGAAAAAGAAGAGGGTAGAA | primer | 5p | 31 | 58 |
AAAAATATCTCCCGCATCATTCAC | primer | 3p | 127 | 150 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR319c found in PubMed |