Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.92227 | 0.341781 | 3.99478 | 0.202045 | |
| 10day-seedling | 2.81183 | 0.429062 | 4.61999 | 0.251319 | |
| 14day-seedling | 2.87362 | 0.220049 | 4.04883 | 0.578117 | |
| 25day-aerial | 2.00309 | 0.606668 | 3.76571 | 0.642766 | |
| 35day-aerial | 2.64923 | 0.171462 | 4.61481 | 0.059087 | |
| 42day-rosette | 2.7686 | 1.08842 | 4.44894 | 1.05887 | 42day-stem | 3.81158 | 0.222647 | 5.5314 | 0.159616 |
| 53day-rosette | 3.70909 | 5.07773 | 5.5516 | 5.01936 | 53day-stem | 4.27011 | 0.608093 | 5.66479 | 0.123899 | 53day-inflores | 3.83872 | 0.434099 | 5.49525 | 0.0375687 | 53day-silique | 3.69732 | 0.356629 | 4.83991 | 0.395645 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 33.87 | 10.02 | 9.64e-4 | -3.02 | 9.93 | 1.02e-3 | -2.99 |
| 2 | 1.000 | 37.18 | 9.21 | 1.69e-3 | -2.77 | 9.34 | 1.54e-3 | -2.81 | |
| 3 | 1.000 | 35.72 | 11.45 | 3.58e-4 | -3.45 | 10.68 | 6.10e-4 | -3.21 | |
| 10day-seedling | 1 | 1.000 | 25.34 | 12.48 | 1.75e-4 | -3.76 | 6.99 | 7.88e-3 | -2.1 |
| 2 | 1.000 | 27.17 | 13.92 | 6.44e-5 | -4.19 | 8.11 | 3.62e-3 | -2.44 | |
| 3 | 1.000 | 26.7 | 15.33 | 2.42e-5 | -4.62 | 8.62 | 2.54e-3 | -2.59 | |
| 14day-seedling | 1 | 1.000 | 27.81 | 14.24 | 5.17e-5 | -4.29 | 7.59 | 5.18e-3 | -2.29 |
| 2 | 0.997 | 32.1 | 13.99 | 6.16e-5 | -4.21 | 11.08 | 4.62e-4 | -3.34 | |
| 3 | 1.000 | 30.66 | 12.87 | 1.33e-4 | -3.88 | 10.73 | 5.87e-4 | -3.23 | |
| 25day-aerial | 1 | 0.999 | 31.78 | 18.93 | 2.01e-6 | -5.7 | 13.17 | 1.09e-4 | -3.96 |
| 2 | 1.000 | 29.42 | 15.38 | 2.35e-5 | -4.63 | 9.14 | 1.78e-3 | -2.75 | |
| 3 | 0.999 | 29.37 | 15.49 | 2.17e-5 | -4.66 | 9.93 | 1.02e-3 | -2.99 | |
| 35day-aerial | 1 | 0.998 | 27.91 | 13.46 | 8.88e-5 | -4.05 | 7.92 | 4.12e-3 | -2.38 |
| 2 | 0.998 | 26.58 | 13.86 | 6.72e-5 | -4.17 | 7.73 | 4.72e-3 | -2.33 | |
| 3 | 0.999 | 27.5 | 14.31 | 4.94e-5 | -4.31 | 8.12 | 3.60e-3 | -2.44 | |
| 42day-rosette | 1 | 0.996 | 24.15 | 10.61 | 6.41e-4 | -3.19 | 3.5 | 8.82e-2 | -1.05 |
| 2 | 1.000 | 32.2 | 17.82 | 4.33e-6 | -5.36 | 12.13 | 2.24e-4 | -3.65 | |
| 3 | 0.998 | 27.24 | 13.72 | 7.40e-5 | -4.13 | 8.15 | 3.51e-3 | -2.45 | |
| 42day-stem | 1 | 1.000 | 21.43 | 9.74 | 1.17e-1 | -2.93 | 4.43 | 4.65e-1 | -1.33 |
| 2 | 0.994 | 23.55 | 11.14 | 4.85e-1 | -3.31 | 5.46 | 2.27e-1 | -1.64 | |
| 3 | 0.998 | 24.41 | 11.27 | 4.79e-1 | -3.32 | 4.72 | 3.79e-1 | -1.43 | |
| 53day-rosette | 1 | 1.000 | 5.85 | -8.2 | 2.95e+2 | 2.47 | -14.19 | 1.87e+4 | 4.27 |
| 2 | 1.000 | 29.64 | 17.35 | 5.97e-6 | -5.22 | 11.64 | 3.14e-4 | -3.5 | |
| 3 | 1.000 | 36.54 | 23.65 | 7.62e-8 | -7.12 | 16.99 | 7.69e-6 | -5.11 | |
| 53day-stem | 1 | 0.999 | 24.62 | 11.2 | 4.26e-4 | -3.37 | 4.68 | 3.91e-2 | -1.41 |
| 2 | 1.000 | 23.59 | 8.82 | 2.21e-3 | -2.65 | 4.17 | 5.57e-2 | -1.25 | |
| 3 | 0.999 | 24.79 | 7.18 | 6.87e-3 | -2.16 | 4.23 | 5.33e-2 | -1.27 | |
| 53day-inflores | 1 | 0.999 | 23.58 | 10.18 | 8.60e-4 | -3.07 | 5.03 | 3.06e-2 | -1.51 |
| 2 | 0.998 | 24.29 | 9.66 | 1.24e-3 | -2.91 | 5.11 | 2.91e-2 | -1.54 | |
| 3 | 1.000 | 23.28 | 9.45 | 1.43e-3 | -2.85 | 4.86 | 3.44e-2 | -1.46 | |
| 53day-silique | 1 | 1.000 | 29.47 | 9.63 | 1.26e-3 | -2.9 | 5.68 | 1.95e-2 | -1.71 |
| 2 | 1.000 | 30.71 | 11.42 | 3.66e-4 | -3.44 | 7.71 | 4.77e-3 | -2.32 | |
| 3 | 1.000 | 30.84 | 11.87 | 2.67e-4 | -3.57 | 8.14 | 3.55e-3 | -2.45 | |
Nothern hybridization
NGS expression data
Expression of miR319a transcript in NGS dataSequences
miRNA transcript
CCAUGCAAAUAUGGAGUAGAUGUACAAACACACGCUCGGACGCAUAUUACACAUGUUCAUACACUUAAUACUCGCUGUUUUGAAUUGAUGUUUUAGGAAUAUAUAUGUAGAGAGAGCUUCCUUGAGUCCAUUCACAGGUCGUGAUAUGAUUCAAUUAGCUUCCGACUCAUUCAUCCAAAUACCGAGUCGCCAAAAUUCAAACUAGACUCGUUAAAUGAAUGAAUGAUGCGGUAGACAAAUUGGAUCAUUGAUUCUCUUUGAUUGGACUGAAGGGAGCUCCCUCU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UUGGACUGAAGGGAGCUCCCU | miRNA | 262 | 282 | |
CCATGCAAATATGGAGTAGATGTACAA | primer | 5p | 1 | 27 |
TGAATGGACTCAAGGAAGCTCTCT | primer | 3p | 111 | 134 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR319a found in PubMed |