Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
Read more
Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.54183 | 0.597116 | 4.61434 | 0.620908 | |
| 10day-seedling | 3.16179 | 0.392843 | 4.96995 | 0.210872 | |
| 14day-seedling | 3.34664 | 0.301809 | 4.51945 | 0.425325 | |
| 25day-aerial | 2.75224 | 0.554251 | 4.26831 | 0.879233 | |
| 35day-aerial | 2.74055 | 0.163943 | 4.68037 | 0.048539 | |
| 42day-rosette | 2.98662 | 0.181238 | 4.60789 | 0.301246 | 42day-stem | 3.04561 | 0.214958 | 4.59033 | 0.168661 |
| 53day-rosette | 3.4362 | 0.272202 | 5.27871 | 0.150179 | 53day-stem | 3.71233 | 0.598508 | 5.23391 | 0.120409 | 53day-inflores | 4.07577 | 0.0244745 | 5.51062 | 0.0828974 | 53day-silique | 3.78493 | 0.0736068 | 4.92752 | 0.074962 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.25 | 10.39 | 7.43e-4 | -3.13 | 10.31 | 7.90e-4 | -3.1 |
| 2 | 1.000 | 34.57 | 6.59 | 1.04e-2 | -1.98 | 6.72 | 9.45e-3 | -2.02 | |
| 3 | 1.000 | 31.79 | 7.51 | 5.48e-3 | -2.26 | 6.74 | 9.33e-3 | -2.03 | |
| 10day-seedling | 1 | 1.000 | 24.35 | 11.49 | 3.48e-4 | -3.46 | 5.99 | 1.57e-2 | -1.8 |
| 2 | 1.000 | 25.92 | 12.67 | 1.54e-4 | -3.81 | 6.86 | 8.63e-3 | -2.06 | |
| 3 | 1.000 | 25.46 | 14.09 | 5.72e-5 | -4.24 | 7.38 | 6.00e-3 | -2.22 | |
| 14day-seedling | 1 | 0.999 | 26.83 | 13.26 | 1.02e-4 | -3.99 | 6.62 | 1.02e-2 | -1.99 |
| 2 | 0.998 | 29.92 | 11.81 | 2.79e-4 | -3.55 | 8.9 | 2.09e-3 | -2.68 | |
| 3 | 0.995 | 29.13 | 11.34 | 3.86e-4 | -3.41 | 9.2 | 1.70e-3 | -2.77 | |
| 25day-aerial | 1 | 1.000 | 26.01 | 13.15 | 1.10e-4 | -3.96 | 7.39 | 5.96e-3 | -2.22 |
| 2 | 0.999 | 30.28 | 16.23 | 1.30e-5 | -4.89 | 9.99 | 9.83e-4 | -3.01 | |
| 3 | 1.000 | 26.83 | 12.95 | 1.27e-4 | -3.9 | 7.39 | 5.98e-3 | -2.22 | |
| 35day-aerial | 1 | 0.995 | 27.76 | 13.3 | 9.90e-5 | -4 | 7.77 | 4.60e-3 | -2.34 |
| 2 | 0.997 | 26.38 | 13.66 | 7.75e-5 | -4.11 | 7.52 | 5.44e-3 | -2.26 | |
| 3 | 1.000 | 27.21 | 14.02 | 6.04e-5 | -4.22 | 7.83 | 4.40e-3 | -2.36 | |
| 42day-rosette | 1 | 0.999 | 27.53 | 13.99 | 6.13e-5 | -4.21 | 6.89 | 8.44e-3 | -2.07 |
| 2 | 0.999 | 27.57 | 13.19 | 1.07e-4 | -3.97 | 7.49 | 5.55e-3 | -2.26 | |
| 3 | 1.000 | 26.34 | 12.82 | 1.39e-4 | -3.86 | 7.25 | 6.57e-3 | -2.18 | |
| 42day-stem | 1 | 0.998 | 24.93 | 13.24 | 1.33e-1 | -3.99 | 7.93 | 4.97e-1 | -2.39 |
| 2 | 0.998 | 24.9 | 12.36 | 1.93e-1 | -3.73 | 6.81 | 8.92e-1 | -2.59 | |
| 3 | 0.999 | 27.18 | 13.79 | 7.53e-1 | -4.15 | 7.48 | 5.58e-1 | -2.25 | |
| 53day-rosette | 1 | 1.000 | 25.26 | 11.21 | 4.22e-4 | -3.37 | 5.22 | 2.68e-2 | -1.57 |
| 2 | 1.000 | 23.72 | 11.43 | 3.62e-4 | -3.44 | 5.72 | 1.90e-2 | -1.72 | |
| 3 | 1.000 | 25.77 | 12.87 | 1.33e-4 | -3.88 | 6.22 | 1.34e-2 | -1.87 | |
| 53day-stem | 1 | 0.996 | 26.07 | 12.65 | 1.55e-4 | -3.81 | 6.13 | 1.42e-2 | -1.85 |
| 2 | 1.000 | 24.83 | 10.06 | 9.37e-4 | -3.03 | 5.41 | 2.36e-2 | -1.63 | |
| 3 | 1.000 | 26.55 | 8.94 | 2.04e-3 | -2.69 | 5.99 | 1.58e-2 | -1.8 | |
| 53day-inflores | 1 | 0.999 | 23.18 | 9.79 | 1.13e-3 | -2.95 | 4.64 | 4.02e-2 | -1.4 |
| 2 | 0.997 | 24.35 | 9.72 | 1.18e-3 | -2.93 | 5.17 | 2.79e-2 | -1.55 | |
| 3 | 0.997 | 23.46 | 9.63 | 1.26e-3 | -2.9 | 5.04 | 3.04e-2 | -1.52 | |
| 53day-silique | 1 | 1.000 | 30.57 | 10.72 | 5.93e-4 | -3.23 | 6.77 | 9.16e-3 | -2.04 |
| 2 | 1.000 | 29.71 | 10.42 | 7.31e-4 | -3.14 | 6.71 | 9.53e-3 | -2.02 | |
| 3 | 1.000 | 29.87 | 10.9 | 5.23e-4 | -3.28 | 7.17 | 6.94e-3 | -2.16 | |
Nothern hybridization
NGS expression data
Expression of miR172e transcript in NGS dataSequences
miRNA transcript
AAGGAUCUUUCUCUGCGGUUGAAAUAGCUAGGUUCUCGAUGAAUAGGCUAGCCUUUGGUGGAUGUUAUCAGCCAGUAGUCGCAGAUGCAGCACCAUUAAGAUUCACAAGAGAUGUGGUUCCCUUUGCUUUCGCCUCUCGAUCCGCAGAAAAGGGUUCCUUAUCGAGUGGGAAUCUUGAUGAUGCUGCAUCAGCAAAUAC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
GGAAUCUUGAUGAUGCUGCAU | miRNA | 169 | 189 | |
AAGGATCTTTCTCTGCGGTTGA | primer | 5p | 1 | 22 |
CTCGATAAGGAACCCTTTTCTGC | primer | 3p | 144 | 166 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR172e found in PubMed |