Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.57922 | 0.0553535 | 4.65173 | 0.145827 | |
| 10day-seedling | 1.1666 | 0.892144 | 2.97476 | 1.01791 | |
| 14day-seedling | 1.71293 | 0.127147 | 3.15326 | 0.918694 | |
| 25day-aerial | 0.12166 | 0 | 1.76222 | 0 | |
| 35day-aerial | 1.41423 | 0.744743 | 3.01874 | 0.956192 | |
| 42day-rosette | 1.38498 | 1.29442 | 3.15842 | 1.79947 | 42day-stem | 3.24519 | 0.127768 | 4.9288 | 0.0979359 |
| 53day-rosette | 1.72478 | 0.217969 | 3.56728 | 0.214838 | 53day-stem | 2.71478 | 1.4978 | 4.13221 | 0.974784 | 53day-inflores | 2.73256 | 0.877706 | 4.10154 | 0.872377 | 53day-silique | 3.78154 | 0.339719 | 4.92413 | 0.371621 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 31.69 | 7.83 | 4.39e-3 | -2.36 | 7.75 | 4.66e-3 | -2.33 |
| 2 | 1.000 | 36.15 | 8.18 | 3.46e-3 | -2.46 | 8.31 | 3.15e-3 | -2.5 | |
| 3 | 1.000 | 32.39 | 8.12 | 3.60e-3 | -2.44 | 7.35 | 6.14e-3 | -2.21 | |
| 10day-seedling | 1 | 1.000 | 35.63 | 22.77 | 1.40e-7 | -6.85 | 17.28 | 6.30e-6 | -5.2 |
| 2 | 1.000 | 30.53 | 17.29 | 6.25e-6 | -5.2 | 11.47 | 3.52e-4 | -3.45 | |
| 3 | 1.000 | 29.45 | 18.08 | 3.61e-6 | -5.44 | 11.37 | 3.79e-4 | -3.42 | |
| 14day-seedling | 1 | 0.997 | 30.84 | 17.26 | 6.35e-6 | -5.2 | 10.62 | 6.35e-4 | -3.2 |
| 2 | 0.999 | 35.95 | 17.84 | 4.26e-6 | -5.37 | 14.94 | 3.19e-5 | -4.5 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 36.73 | 22.85 | 1.32e-7 | -6.88 | 17.29 | 6.26e-6 | -5.2 | |
| 35day-aerial | 1 | 0.995 | 34.44 | 19.98 | 9.66e-7 | -6.02 | 14.45 | 4.48e-5 | -4.35 |
| 2 | 0.999 | 28.48 | 15.75 | 1.81e-5 | -4.74 | 9.62 | 1.27e-3 | -2.9 | |
| 3 | 0.998 | 32.67 | 19.48 | 1.37e-6 | -5.86 | 13.29 | 9.99e-5 | -4 | |
| 42day-rosette | 1 | 0.998 | 26.89 | 13.35 | 9.57e-5 | -4.02 | 6.25 | 1.32e-2 | -1.88 |
| 2 | 0.998 | 34.1 | 19.72 | 1.16e-6 | -5.94 | 14.03 | 5.97e-5 | -4.22 | |
| 3 | 0.999 | 35.84 | 22.32 | 1.91e-7 | -6.72 | 16.75 | 9.08e-6 | -5.04 | |
| 42day-stem | 1 | 0.995 | 23.69 | 12 | 2.45e-1 | -3.61 | 6.69 | 9.76e-1 | -2.13 |
| 2 | 0.999 | 25.15 | 12.62 | 1.60e-1 | -3.8 | 7.62 | 7.49e-1 | -2.13 | |
| 3 | 0.998 | 26.19 | 12.81 | 1.40e-1 | -3.86 | 6.55 | 1.11e-1 | -1.96 | |
| 53day-rosette | 1 | 1.000 | 30.74 | 16.69 | 9.43e-6 | -5.03 | 10.71 | 5.99e-4 | -3.22 |
| 2 | 1.000 | 30.13 | 17.85 | 4.24e-6 | -5.37 | 12.13 | 2.23e-4 | -3.65 | |
| 3 | 1.000 | 30.92 | 18.03 | 3.74e-6 | -5.43 | 11.37 | 3.77e-4 | -3.42 | |
| 53day-stem | 1 | 0.997 | 32.13 | 18.71 | 2.34e-6 | -5.63 | 12.19 | 2.14e-4 | -3.67 |
| 2 | 0.999 | 29.9 | 15.12 | 2.80e-5 | -4.55 | 10.47 | 7.05e-4 | -3.15 | |
| 3 | 0.999 | 26.48 | 8.88 | 2.13e-3 | -2.67 | 5.92 | 1.65e-2 | -1.78 | |
| 53day-inflores | 1 | 0.995 | 25.26 | 11.87 | 2.67e-4 | -3.57 | 6.72 | 9.51e-3 | -2.02 |
| 2 | 0.998 | 32.08 | 17.45 | 5.57e-6 | -5.25 | 12.9 | 1.31e-4 | -3.88 | |
| 3 | 0.995 | 27.03 | 13.2 | 1.06e-4 | -3.97 | 8.61 | 2.55e-3 | -2.59 | |
| 53day-silique | 1 | 1.000 | 29.63 | 9.79 | 1.13e-3 | -2.95 | 5.84 | 1.75e-2 | -1.76 |
| 2 | 1.000 | 31.25 | 11.96 | 2.52e-4 | -3.6 | 8.25 | 3.28e-3 | -2.48 | |
| 3 | 1.000 | 29.3 | 10.33 | 7.77e-4 | -3.11 | 6.6 | 1.03e-2 | -1.99 | |
Nothern hybridization
NGS expression data
Expression of miR172c transcript in NGS dataSequences
miRNA transcript
AGCUACUGUUCGCUGUUGGAGCAUCAUCAAGAUUCACAAAUCAUCAAGUAUUCGUGUAAAUAAACCCAUUUAUGAUUAGAUUUUUGAUGUAUGUAUGAGAAUCUUGAUGAUGCUGCAGCUGCAAUCAGUGGCU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
AGAAUCUUGAUGAUGCUGCAG | miRNA | 98 | 118 | |
TTCGCTGTTGGAGCATCAT | primer | 5p | 9 | 27 |
GCAGCTGCAGCATCATCA | primer | 3p | 105 | 122 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR172c found in PubMed |