Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.0037 | 1.28822 | 4.07622 | 1.16002 | |
| 10day-seedling | 3.35151 | 0.868888 | 5.15966 | 0.745525 | |
| 14day-seedling | 3.66318 | 0.565385 | 4.83582 | 0.212477 | |
| 25day-aerial | 4.011 | 0.417239 | 5.79076 | 0.282983 | |
| 35day-aerial | 4.36895 | 0.367538 | 5.90609 | 0.694582 | |
| 42day-rosette | 4.67654 | 0.512842 | 6.545748 | 0.8157 | 42day-stem | 5.36085 | 0.0765139 | 7.0171711 | 0.288668 |
| 53day-rosette | 4.61124 | 0.524938 | 6.453747 | 0.493653 | 53day-stem | 5.11216 | 0.585159 | 6.482239 | 1.0357 | 53day-inflores | 5.16817 | 0.146951 | 6.556929 | 0.223642 | 53day-silique | 3.84446 | 0.371411 | 4.98705 | 0.398602 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.45 | 10.59 | 6.48e-4 | -3.19 | 10.51 | 6.88e-4 | -3.16 |
| 2 | 1.000 | 33.37 | 5.39 | 2.38e-2 | -1.62 | 5.52 | 2.17e-2 | -1.66 | |
| 3 | 1.000 | 38.15 | 13.88 | 6.64e-5 | -4.18 | 13.11 | 1.13e-4 | -3.95 | |
| 10day-seedling | 1 | 1.000 | 21.65 | 8.79 | 2.26e-3 | -2.65 | 3.29 | 1.02e-1 | -0.99 |
| 2 | 1.000 | 27 | 13.75 | 7.27e-5 | -4.14 | 7.93 | 4.09e-3 | -2.39 | |
| 3 | 1.000 | 25.19 | 13.83 | 6.89e-5 | -4.16 | 7.11 | 7.23e-3 | -2.14 | |
| 14day-seedling | 1 | 0.997 | 26.67 | 13.1 | 1.14e-4 | -3.94 | 6.46 | 1.14e-2 | -1.94 |
| 2 | 0.984 | 28.88 | 10.77 | 5.73e-4 | -3.24 | 7.86 | 4.29e-3 | -2.37 | |
| 3 | 0.994 | 27.17 | 9.38 | 1.50e-3 | -2.82 | 7.25 | 6.59e-3 | -2.18 | |
| 25day-aerial | 1 | 0.996 | 22.33 | 9.48 | 1.41e-3 | -2.85 | 3.72 | 7.61e-2 | -1.12 |
| 2 | 0.998 | 25.36 | 11.31 | 3.93e-4 | -3.41 | 5.07 | 2.98e-2 | -1.53 | |
| 3 | 0.999 | 22.71 | 8.83 | 2.20e-3 | -2.66 | 3.26 | 1.04e-1 | -0.98 | |
| 35day-aerial | 1 | 0.998 | 21.77 | 7.32 | 6.28e-3 | -2.2 | 1.78 | 2.91e-1 | -0.54 |
| 2 | 0.998 | 22.47 | 9.74 | 1.17e-3 | -2.93 | 3.61 | 8.18e-2 | -1.09 | |
| 3 | 0.000 | 22.29 | 9.09 | 1.83e-3 | -2.74 | 2.9 | 1.34e-1 | -0.87 | |
| 42day-rosette | 1 | 0.999 | 19.29 | 5.75 | 1.86e-2 | -1.73 | -1.36 | 2.56e+0 | 0.41 |
| 2 | 0.998 | 22.95 | 8.57 | 2.64e-3 | -2.58 | 2.87 | 1.36e-1 | -0.86 | |
| 3 | 1.000 | 22.36 | 8.83 | 2.19e-3 | -2.66 | 3.27 | 1.04e-1 | -0.98 | |
| 42day-stem | 1 | 0.998 | 17.11 | 5.41 | 2.34e-1 | -1.64 | 0.15 | 9.30e-1 | -0.32 |
| 2 | 0.998 | 17.74 | 5.19 | 2.73e-1 | -1.56 | -0.36 | 1.28e+0 | 0.17 | |
| 3 | 0.999 | 19.03 | 5.65 | 1.99e-1 | -1.72 | -0.66 | 1.58e+0 | 0.2 | |
| 53day-rosette | 1 | 1.000 | 19.98 | 5.94 | 1.63e-2 | -1.79 | -0.05 | 1.04e+0 | 0.02 |
| 2 | 1.000 | 21.02 | 8.73 | 2.35e-3 | -2.63 | 3.02 | 1.24e-1 | -0.91 | |
| 3 | 1.000 | 22.03 | 9.14 | 1.77e-3 | -2.75 | 2.48 | 1.79e-1 | -0.75 | |
| 53day-stem | 1 | 0.998 | 19.78 | 6.36 | 1.22e-2 | -1.91 | -0.16 | 1.12e+0 | 0.05 |
| 2 | 0.998 | 22.94 | 8.17 | 3.47e-3 | -2.46 | 3.52 | 8.74e-2 | -1.06 | |
| 3 | 1.000 | 21.89 | 4.28 | 5.13e-2 | -1.29 | 1.33 | 3.98e-1 | -0.4 | |
| 53day-inflores | 1 | 0.997 | 19.46 | 6.07 | 1.49e-2 | -1.83 | 0.91 | 5.31e-1 | -0.28 |
| 2 | 1.000 | 21.21 | 6.58 | 1.04e-2 | -1.98 | 2.03 | 2.46e-1 | -0.61 | |
| 3 | 1.000 | 19.43 | 5.61 | 2.05e-2 | -1.69 | 1.02 | 4.95e-1 | -0.31 | |
| 53day-silique | 1 | 1.000 | 29.53 | 9.69 | 1.21e-3 | -2.92 | 5.74 | 1.88e-2 | -1.73 |
| 2 | 1.000 | 31.2 | 11.9 | 2.61e-4 | -3.58 | 8.2 | 3.40e-3 | -2.47 | |
| 3 | 1.000 | 28.83 | 9.86 | 1.08e-3 | -2.97 | 6.12 | 1.43e-2 | -1.84 | |
Nothern hybridization
NGS expression data
Expression of miR172b transcript in NGS dataSequences
miRNA transcript
UAGGCGCAGCACCAUUAAGAUUCACAUGGAAAUUGAUAAAUACCCUAAAUUAGGGUUUUGAUAUGUAUAUGAGAAUCUUGAUGAUGCUGCAUCAACAAUCGACGGCUACAAA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
AGAAUCUUGAUGAUGCUGCAU | miRNA | 72 | 92 | |
TAGGCGCAGCACCATTAAGATTCAC | primer | 5p | 1 | 25 |
TTGTAGCCGTCGATTGTTG | primer | 3p | 93 | 111 |
GCAGCACCAUUAAGAUUCAC | miRNA* | * | 6 | 25 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR172b found in PubMed |