Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.09484 | 0.51014 | 4.16736 | 0.396185 | |
| 10day-seedling | 3.47502 | 0.743616 | 5.28318 | 0.603011 | |
| 14day-seedling | 3.40397 | 0.151031 | 4.57678 | 0.575669 | |
| 25day-aerial | 3.58754 | 0.970072 | 5.31164 | 0.873243 | |
| 35day-aerial | 3.47833 | 0.13566 | 5.60548 | 0.184383 | |
| 42day-rosette | 3.82825 | 0.214633 | 5.37649 | 0.380967 | 42day-stem | 3.82275 | 0.278525 | 5.65268 | 0.265868 |
| 53day-rosette | 5.12113 | 0.525027 | 6.9636294 | 0.527095 | 53day-stem | 4.84394 | 0.526843 | 6.258562 | 0.154738 | 53day-inflores | 4.30367 | 0.10404 | 5.74048 | 0.116876 | 53day-silique | 4.058 | 0.482639 | 5.2006 | 0.521178 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.01 | 10.16 | 8.76e-4 | -3.06 | 10.07 | 9.30e-4 | -3.03 |
| 2 | 1.000 | 35.74 | 7.76 | 4.61e-3 | -2.34 | 7.89 | 4.20e-3 | -2.38 | |
| 3 | 1.000 | 35.31 | 11.03 | 4.77e-4 | -3.32 | 10.26 | 8.13e-4 | -3.09 | |
| 10day-seedling | 1 | 1.000 | 21.75 | 8.89 | 2.11e-3 | -2.68 | 3.39 | 9.52e-2 | -1.02 |
| 2 | 1.000 | 26.02 | 12.77 | 1.43e-4 | -3.84 | 6.95 | 8.06e-3 | -2.09 | |
| 3 | 1.000 | 24.84 | 13.48 | 8.78e-5 | -4.06 | 6.76 | 9.21e-3 | -2.04 | |
| 14day-seedling | 1 | 0.999 | 26.08 | 12.5 | 1.72e-4 | -3.76 | 5.86 | 1.72e-2 | -1.76 |
| 2 | 0.999 | 29.91 | 11.8 | 2.81e-4 | -3.55 | 8.89 | 2.10e-3 | -2.68 | |
| 3 | 0.999 | 29.32 | 11.54 | 3.37e-4 | -3.47 | 9.4 | 1.48e-3 | -2.83 | |
| 25day-aerial | 1 | 0.998 | 20.97 | 8.11 | 3.61e-3 | -2.44 | 2.36 | 1.95e-1 | -0.71 |
| 2 | 1.000 | 28.6 | 14.56 | 4.14e-5 | -4.38 | 8.32 | 3.14e-3 | -2.5 | |
| 3 | 1.000 | 25.21 | 11.33 | 3.88e-4 | -3.41 | 5.77 | 1.83e-2 | -1.74 | |
| 35day-aerial | 1 | 0.898 | 24.55 | 10.1 | 9.11e-4 | -3.04 | 4.56 | 4.23e-2 | -1.37 |
| 2 | 0.999 | 22.91 | 10.19 | 8.56e-4 | -3.07 | 4.06 | 6.00e-2 | -1.22 | |
| 3 | 0.999 | 24.66 | 11.46 | 3.54e-4 | -3.45 | 5.28 | 2.58e-2 | -1.59 | |
| 42day-rosette | 1 | 0.992 | 24.29 | 10.75 | 5.80e-4 | -3.24 | 3.65 | 7.99e-2 | -1.1 |
| 2 | 0.999 | 24.12 | 9.74 | 1.17e-3 | -2.93 | 4.05 | 6.04e-2 | -1.22 | |
| 3 | 0.999 | 24.64 | 11.12 | 4.50e-4 | -3.35 | 5.55 | 2.13e-2 | -1.67 | |
| 42day-stem | 1 | 0.999 | 21.18 | 9.49 | 1.39e-1 | -2.86 | 4.18 | 5.52e-1 | -1.26 |
| 2 | 0.997 | 23.56 | 11.18 | 4.82e-1 | -3.32 | 5.47 | 2.26e-1 | -1.65 | |
| 3 | 1.000 | 23.47 | 1.85 | 9.28e-1 | -3.36 | 3.78 | 7.29e-1 | -1.14 | |
| 53day-rosette | 1 | 1.000 | 18.29 | 4.24 | 5.29e-2 | -1.28 | -1.75 | 3.36e+0 | 0.53 |
| 2 | 1.000 | 19.73 | 7.44 | 5.76e-3 | -2.24 | 1.72 | 3.03e-1 | -0.52 | |
| 3 | 1.000 | 19.94 | 7.05 | 7.57e-3 | -2.12 | 0.39 | 7.64e-1 | -0.12 | |
| 53day-stem | 1 | 0.999 | 22 | 8.58 | 2.62e-3 | -2.58 | 2.06 | 2.40e-1 | -0.62 |
| 2 | 1.000 | 22.48 | 7.7 | 4.80e-3 | -2.32 | 3.05 | 1.21e-1 | -0.92 | |
| 3 | 1.000 | 22.81 | 5.21 | 2.71e-2 | -1.57 | 2.25 | 2.10e-1 | -0.68 | |
| 53day-inflores | 1 | 1.000 | 22.5 | 9.1 | 1.82e-3 | -2.74 | 3.95 | 6.47e-2 | -1.19 |
| 2 | 0.999 | 23.82 | 9.19 | 1.71e-3 | -2.77 | 4.63 | 4.03e-2 | -1.39 | |
| 3 | 1.000 | 22.39 | 8.56 | 2.65e-3 | -2.58 | 3.97 | 6.38e-2 | -1.2 | |
| 53day-silique | 1 | 1.000 | 27.94 | 8.09 | 3.66e-3 | -2.44 | 4.14 | 5.66e-2 | -1.25 |
| 2 | 1.000 | 30.58 | 11.29 | 4.00e-4 | -3.4 | 7.58 | 5.22e-3 | -2.28 | |
| 3 | 1.000 | 28.91 | 9.94 | 1.02e-3 | -2.99 | 6.21 | 1.35e-2 | -1.87 | |
Nothern hybridization
NGS expression data
Expression of miR172a transcript in NGS dataSequences
miRNA transcript
UGCUGUGGCAUCAUCAAGAUUCACAUCUGUUGAUGGACGGUGGUGAUUCACUCUCCACAAAGUUCUCUAUGAAAAUGAGAAUCUUGAUGAUGCUGCAUCGGCAAUCAACGACUAUUCUUUCCCUCUCUCUCUCUCUCCCUCUGUAUAGAUUAUUUGGAUUCCAUCCAGAUCUUCUUCAGGU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
AGAAUCUUGAUGAUGCUGCAU | miRNA | 78 | 98 | |
CTGTTGATGGACGGTGGTGAT | primer | 5p | 27 | 47 |
CCTGAAGAAGATCTGGATGGAATC | primer | 3p | 157 | 180 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR172a found in PubMed |