Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 2.65328 | 0.726867 | 2.72579 | 0.586321 | |
| 10day-seedling | 3.32721 | 0.163183 | 5.13536 | 0.240637 | |
| 14day-seedling | 3.85714 | 0.390165 | 4.88017 | 0.679492 | |
| 25day-aerial | 3.57047 | 0 | 5.44764 | 0 | |
| 35day-aerial | 3.07175 | 0.217384 | 4.93752 | 0.262538 | |
| 42day-rosette | 2.03084 | 0.780373 | 4.20266 | 0.695298 | 42day-stem | 1.97798 | 0.640842 | 3.86259 | 0.667768 |
| 53day-rosette | 1.01704 | 0.18918 | 2.87936 | 0.389481 | 53day-stem | 1.15853 | 1.93054 | 2.57683 | 1.49956 | 53day-inflores | 1.60526 | 0.40107 | 2.95107 | 0.451869 | 53day-silique | 2.52882 | 1.15726 | 3.67142 | 1.19675 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 37.2 | 13.34 | 9.62e-5 | -4.02 | 13.26 | 1.02e-4 | -3.99 |
| 2 | 1.000 | 40.74 | 12.77 | 1.43e-4 | -3.84 | 12.9 | 1.31e-4 | -3.88 | |
| 3 | 1.000 | 41.48 | 17.21 | 6.61e-6 | -5.18 | 16.44 | 1.13e-5 | -4.95 | |
| 10day-seedling | 1 | 1.000 | 25.47 | 12.61 | 1.60e-4 | -3.8 | 7.12 | 7.21e-3 | -2.14 |
| 2 | 1.000 | 24.83 | 11.59 | 3.25e-4 | -3.49 | 5.77 | 1.83e-2 | -1.74 | |
| 3 | 1.000 | 23.78 | 12.41 | 1.84e-4 | -3.73 | 5.69 | 1.93e-2 | -1.71 | |
| 14day-seedling | 1 | 1.000 | 24.65 | 11.08 | 4.62e-4 | -3.34 | 4.44 | 4.62e-2 | -1.34 |
| 2 | 1.000 | 29.4 | 11.29 | 3.99e-4 | -3.4 | 8.39 | 2.99e-3 | -2.52 | |
| 3 | 0.997 | 26.74 | 8.95 | 2.02e-3 | -2.69 | 6.81 | 8.91e-3 | -2.05 | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.998 | 25.44 | 11.39 | 3.72e-4 | -3.43 | 5.15 | 2.82e-2 | -1.55 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | 1.000 | 27.8 | 13.35 | 9.60e-5 | -4.02 | 7.81 | 4.46e-3 | -2.35 |
| 2 | 1.000 | 25.04 | 12.32 | 1.96e-4 | -3.71 | 6.18 | 1.38e-2 | -1.86 | |
| 3 | 0.995 | 25.91 | 12.72 | 1.48e-4 | -3.83 | 6.53 | 1.08e-2 | -1.97 | |
| 42day-rosette | 1 | 1.000 | 32.59 | 19.05 | 1.84e-6 | -5.74 | 11.95 | 2.53e-4 | -3.6 |
| 2 | 1.000 | 28.25 | 13.87 | 6.68e-5 | -4.18 | 8.18 | 3.45e-3 | -2.46 | |
| 3 | 1.000 | 26.84 | 13.32 | 9.79e-5 | -4.01 | 7.75 | 4.64e-3 | -2.33 | |
| 42day-stem | 1 | 1.000 | 29.24 | 17.55 | 5.21e-6 | -5.28 | 12.24 | 2.68e-1 | -3.68 |
| 2 | 1.000 | 29.17 | 16.63 | 9.89e-1 | -5.49 | 11.77 | 4.63e-1 | -3.33 | |
| 3 | 0.998 | 27.64 | 14.26 | 5.17e-1 | -4.29 | 7.95 | 4.43e-1 | -2.39 | |
| 53day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 32.61 | 20.32 | 7.64e-7 | -6.12 | 14.6 | 4.02e-5 | -4.4 | |
| 3 | 1.000 | 32.32 | 19.43 | 1.42e-6 | -5.85 | 12.77 | 1.43e-4 | -3.85 | |
| 53day-stem | 1 | 1.000 | 36.51 | 23.09 | 1.12e-7 | -6.95 | 16.57 | 1.03e-5 | -4.99 |
| 2 | 1.000 | 37.89 | 23.12 | 1.10e-7 | -6.96 | 18.47 | 2.76e-6 | -5.56 | |
| 3 | 1.000 | 29.6 | 12 | 2.44e-4 | -3.61 | 9.05 | 1.89e-3 | -2.72 | |
| 53day-inflores | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 33.49 | 18.86 | 2.10e-6 | -5.68 | 14.31 | 4.93e-5 | -4.31 | |
| 3 | 0.988 | 30.81 | 16.98 | 7.74e-6 | -5.11 | 12.39 | 1.86e-4 | -3.73 | |
| 53day-silique | 1 | 1.000 | 30.3 | 10.45 | 7.13e-4 | -3.15 | 6.5 | 1.10e-2 | -1.96 |
| 2 | 1.000 | 35.82 | 16.53 | 1.05e-5 | -4.98 | 12.83 | 1.38e-4 | -3.86 | |
| 3 | 1.000 | 36.54 | 17.57 | 5.13e-6 | -5.29 | 13.84 | 6.82e-5 | -4.17 | |
NGS expression data
Expression of miR169n transcript in NGS dataSequences
miRNA transcript
GAUGAAGAAGAGAGGUCUAACAUGGCGGAAAGCGUCAUGUUUAGUAGCCAAGGAUGACUUGCCUGAUCUUUUUCGCCUCCACGAUUCAAUUUCAAAUUCAUGCAUUUUGGAUUAUUAUACCUUUUAAAGUAUAAUAGGUCAAAUAUCAUGUUGAAUCUUGCGGGUUAGGUUUCAGGCAGUCUCUUUGGCUAUCUUGACAUGCUUUUUCCAUCCAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAGCCAAGGAUGACUUGCCUG | miRNA | 45 | 65 | |
TCGCCTCCACGATTCAATTT | primer | 5p | 73 | 92 |
GATAGCCAAAGAGACTGCCTGAAA | primer | 3p | 170 | 193 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169n found in PubMed |