Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.67642 | 1.10253 | 3.74893 | 1.24427 | |
| 10day-seedling | 3.67339 | 0.275474 | 5.48155 | 0.368223 | |
| 14day-seedling | 4.28866 | 0.129866 | 5.46448 | 0.61768 | |
| 25day-aerial | 4.36843 | 0.19427 | 6.104162 | 0.136367 | |
| 35day-aerial | 3.60506 | 0.418984 | 5.65704 | 0.146777 | |
| 42day-rosette | 3.11985 | 0.44347 | 4.71184 | 0.456117 | 42day-stem | 2.64502 | 0.261675 | 4.2112 | 0.638907 |
| 53day-rosette | 1.96635 | 0.805764 | 3.80886 | 0.821201 | 53day-stem | 2.28902 | 0.994033 | 3.70678 | 0.626586 | 53day-inflores | 1.61577 | 0.387126 | 3.26454 | 0.19633 | 53day-silique | 3.61986 | 0.737985 | 4.76246 | 0.777518 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 36.08 | 12.22 | 2.10e-4 | -3.68 | 12.13 | 2.23e-4 | -3.65 |
| 2 | 1.000 | 41.95 | 13.97 | 6.24e-5 | -4.2 | 14.1 | 5.69e-5 | -4.25 | |
| 3 | 1.000 | 31.21 | 6.93 | 8.18e-3 | -2.09 | 6.16 | 1.39e-2 | -1.86 | |
| 10day-seedling | 1 | 1.000 | 24.81 | 11.95 | 2.53e-4 | -3.6 | 6.46 | 1.14e-2 | -1.94 |
| 2 | 1.000 | 23.37 | 10.12 | 8.98e-4 | -3.05 | 4.31 | 5.05e-2 | -1.3 | |
| 3 | 1.000 | 22.45 | 11.08 | 4.62e-4 | -3.34 | 4.37 | 4.84e-2 | -1.32 | |
| 14day-seedling | 1 | 1.000 | 22.95 | 9.38 | 1.50e-3 | -2.82 | 2.73 | 1.50e-1 | -0.82 |
| 2 | 0.999 | 27.22 | 9.11 | 1.82e-3 | -2.74 | 6.2 | 1.36e-2 | -1.87 | |
| 3 | 0.999 | 26.3 | 8.51 | 2.75e-3 | -2.56 | 6.37 | 1.21e-2 | -1.92 | |
| 25day-aerial | 1 | 1.000 | 21.8 | 8.95 | 2.03e-3 | -2.69 | 3.19 | 1.10e-1 | -0.96 |
| 2 | 0.997 | 23.3 | 9.26 | 1.63e-3 | -2.79 | 3.02 | 1.24e-1 | -0.91 | |
| 3 | 1.000 | 21.9 | 8.02 | 3.86e-3 | -2.41 | 2.46 | 1.82e-1 | -0.74 | |
| 35day-aerial | 1 | 0.999 | 24.98 | 10.52 | 6.79e-4 | -3.17 | 4.99 | 3.15e-2 | -1.5 |
| 2 | 0.997 | 22.98 | 10.26 | 8.17e-4 | -3.09 | 4.12 | 5.73e-2 | -1.24 | |
| 3 | 1.000 | 23.62 | 10.43 | 7.27e-4 | -3.14 | 4.24 | 5.30e-2 | -1.28 | |
| 42day-rosette | 1 | 1.000 | 28.09 | 14.55 | 4.18e-5 | -4.38 | 7.44 | 5.75e-3 | -2.24 |
| 2 | 1.000 | 26.74 | 12.36 | 1.90e-4 | -3.72 | 6.67 | 9.83e-3 | -2.01 | |
| 3 | 0.999 | 25.25 | 11.73 | 2.94e-4 | -3.53 | 6.16 | 1.39e-2 | -1.86 | |
| 42day-stem | 1 | 1.000 | 27.13 | 15.44 | 2.25e-1 | -4.65 | 1.13 | 8.93e-1 | -3.49 |
| 2 | 1.000 | 26.74 | 14.2 | 5.33e-1 | -4.27 | 8.65 | 2.50e-1 | -2.63 | |
| 3 | 0.999 | 27.15 | 13.77 | 7.18e-1 | -4.14 | 7.46 | 5.68e-1 | -2.25 | |
| 53day-rosette | 1 | 1.000 | 27.78 | 13.73 | 7.36e-5 | -4.13 | 7.74 | 4.67e-3 | -2.33 |
| 2 | 1.000 | 31.18 | 18.89 | 2.06e-6 | -5.69 | 13.17 | 1.08e-4 | -3.97 | |
| 3 | 1.000 | 30.43 | 17.54 | 5.23e-6 | -5.28 | 10.89 | 5.28e-4 | -3.28 | |
| 53day-stem | 1 | 1.000 | 30.81 | 17.38 | 5.85e-6 | -5.23 | 10.86 | 5.36e-4 | -3.27 |
| 2 | 1.000 | 32.39 | 17.62 | 4.97e-6 | -5.3 | 12.96 | 1.25e-4 | -3.9 | |
| 3 | 1.000 | 29.45 | 11.84 | 2.73e-4 | -3.56 | 8.89 | 2.11e-3 | -2.68 | |
| 53day-inflores | 1 | 0.999 | 31.66 | 18.27 | 3.16e-6 | -5.5 | 13.12 | 1.12e-4 | -3.95 |
| 2 | 1.000 | 31.46 | 16.83 | 8.59e-6 | -5.07 | 12.27 | 2.02e-4 | -3.69 | |
| 3 | 0.991 | 30.25 | 16.42 | 1.14e-5 | -4.94 | 11.83 | 2.75e-4 | -3.56 | |
| 53day-silique | 1 | 1.000 | 28.27 | 8.42 | 2.91e-3 | -2.54 | 4.47 | 4.51e-2 | -1.35 |
| 2 | 1.000 | 31.6 | 12.31 | 1.97e-4 | -3.71 | 8.6 | 2.57e-3 | -2.59 | |
| 3 | 1.000 | 31.93 | 12.96 | 1.26e-4 | -3.9 | 9.22 | 1.67e-3 | -2.78 | |
NGS expression data
Expression of miR169l transcript in NGS dataSequences
miRNA transcript
AUGAAGAAGAGAGGUCUAAUAUGGCGAAAAGAGUCAUGUUUAAUAGCCAAGGAUGACUUGCCUGAUCUUUUUCACCUCCAUGAUUCAAUUUUAAGUUCGUGGAUUUUGGAUUAUUAUGCGUUUAAAAGGUAUAAUAAUUUGAGAUCAUGUUGAAUCUUGCGGGUUAGGUUUCAGGCAGUCUCUUUGGCUAUCUUGACAUGCUUUCUUCAUC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAGCCAAGGAUGACUUGCCUG | miRNA | 44 | 64 | |
AATAGCCAAGGATGACTTGCCTGATC | primer | 5p | 42 | 67 |
AGAGACTGCCTGAAACCTAACCCG | primer | 3p | 160 | 183 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169l found in PubMed |