Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.06161 | 0.927845 | 3.13412 | 1.06589 | |
| 10day-seedling | 3.42866 | 0.0209545 | 5.23681 | 0.209735 | |
| 14day-seedling | 2.79581 | 1.14562 | 3.96888 | 1.79739 | |
| 25day-aerial | 4.02183 | 0.557717 | 5.75614 | 0.557666 | |
| 35day-aerial | 4.18093 | 0.1338 | 5.61165 | 0.473242 | |
| 42day-rosette | 2.8531 | 0.534693 | 4.70506 | 0.526508 | 42day-stem | 2.47645 | 0.485599 | 4.29997 | 0.406486 |
| 53day-rosette | 1.71313 | 0.785235 | 3.55563 | 0.758977 | 53day-stem | 1.35677 | 0.220965 | 2.79725 | 0.595069 | 53day-inflores | 1.70788 | 0.528343 | 3.13827 | 0.454328 | 53day-silique | 3.06059 | 0.262493 | 4.20319 | 0.295564 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 38.75 | 14.89 | 3.29e-5 | -4.48 | 14.8 | 3.50e-5 | -4.46 |
| 2 | 1.000 | 42.81 | 14.83 | 3.42e-5 | -4.47 | 14.97 | 3.12e-5 | -4.51 | |
| 3 | 1.000 | 33.8 | 9.52 | 1.36e-3 | -2.87 | 8.75 | 2.32e-3 | -2.64 | |
| 10day-seedling | 1 | 1.000 | 24.75 | 11.89 | 2.63e-4 | -3.58 | 6.4 | 1.18e-2 | -1.93 |
| 2 | 1.000 | 25.16 | 11.91 | 2.59e-4 | -3.59 | 6.1 | 1.46e-2 | -1.84 | |
| 3 | 1.000 | 23.15 | 11.78 | 2.84e-4 | -3.55 | 5.07 | 2.97e-2 | -1.53 | |
| 14day-seedling | 1 | 0.999 | 23.52 | 9.95 | 1.01e-3 | -3 | 3.31 | 1.01e-1 | -1 |
| 2 | 0.985 | 35.63 | 17.52 | 5.32e-6 | -5.27 | 14.62 | 3.98e-5 | -4.4 | |
| 3 | 0.996 | 32.21 | 14.42 | 4.55e-5 | -4.34 | 12.28 | 2.00e-4 | -3.7 | |
| 25day-aerial | 1 | 0.999 | 24.87 | 12.01 | 2.42e-4 | -3.62 | 6.25 | 1.31e-2 | -1.88 |
| 2 | 0.998 | 23.13 | 9.08 | 1.85e-3 | -2.73 | 2.84 | 1.40e-1 | -0.85 | |
| 3 | 1.000 | 22.46 | 8.58 | 2.61e-3 | -2.58 | 3.02 | 1.23e-1 | -0.91 | |
| 35day-aerial | 1 | 0.996 | 23.59 | 9.13 | 1.78e-3 | -2.75 | 3.6 | 8.26e-2 | -1.08 |
| 2 | 0.997 | 21.8 | 9.08 | 1.84e-3 | -2.73 | 2.95 | 1.29e-1 | -0.89 | |
| 3 | 1.000 | 22.85 | 9.66 | 1.24e-3 | -2.91 | 3.47 | 9.02e-2 | -1.04 | |
| 42day-rosette | 1 | 0.999 | 27.66 | 14.12 | 5.62e-5 | -4.25 | 7.01 | 7.74e-3 | -2.11 |
| 2 | 0.996 | 29.67 | 15.29 | 2.50e-5 | -4.6 | 9.6 | 1.29e-3 | -2.89 | |
| 3 | 1.000 | 25.35 | 11.83 | 2.75e-4 | -3.56 | 6.26 | 1.31e-2 | -1.88 | |
| 42day-stem | 1 | 0.998 | 27.43 | 15.75 | 1.83e-1 | -4.74 | 1.43 | 7.25e-1 | -3.14 |
| 2 | 0.997 | 25.86 | 13.32 | 9.83e-1 | -4.86 | 7.77 | 4.59e-1 | -2.34 | |
| 3 | 0.999 | 26.54 | 13.16 | 1.95e-1 | -3.97 | 6.85 | 8.67e-1 | -2.62 | |
| 53day-rosette | 1 | 1.000 | 28.6 | 14.55 | 4.16e-5 | -4.38 | 8.56 | 2.64e-3 | -2.58 |
| 2 | 1.000 | 31.26 | 18.97 | 1.94e-6 | -5.71 | 13.26 | 1.02e-4 | -3.99 | |
| 3 | 1.000 | 32.05 | 19.16 | 1.71e-6 | -5.77 | 12.5 | 1.72e-4 | -3.76 | |
| 53day-stem | 1 | 1.000 | 32.38 | 18.96 | 1.96e-6 | -5.71 | 12.44 | 1.80e-4 | -3.75 |
| 2 | 1.000 | 32.67 | 17.9 | 4.09e-6 | -5.39 | 13.25 | 1.03e-4 | -3.99 | |
| 3 | 9.999 | 36.76 | 19.15 | 1.72e-6 | -5.76 | 16.2 | 1.33e-5 | -4.88 | |
| 53day-inflores | 1 | 1.000 | 32.6 | 19.21 | 1.65e-6 | -5.78 | 14.05 | 5.88e-5 | -4.23 |
| 2 | 1.000 | 32.44 | 17.81 | 4.35e-6 | -5.36 | 13.25 | 1.02e-4 | -3.99 | |
| 3 | 0.999 | 29.55 | 15.72 | 1.85e-5 | -4.73 | 11.13 | 4.46e-4 | -3.35 | |
| 53day-silique | 1 | 1.000 | 32.08 | 12.23 | 2.08e-4 | -3.68 | 8.28 | 3.21e-3 | -2.49 |
| 2 | 1.000 | 32.34 | 13.05 | 1.18e-4 | -3.93 | 9.35 | 1.54e-3 | -2.81 | |
| 3 | 1.000 | 32.95 | 13.98 | 6.21e-5 | -4.21 | 10.24 | 8.25e-4 | -3.08 | |
NGS expression data
Expression of miR169k transcript in NGS dataSequences
miRNA transcript
UUAGAAGGAGAUGUCAAAGAUGAAUAGGAGAACAAUAUUUGGUAGCCAAGGAUGACUUGCCUGCUUCUCUGAACAAAAUGGUCGAUGUCAUGUUUUGAAGUGACUAUAAGUUAUACCAAGAAAUGACCAUUUUGUUUAUAAAUAGACAUCAGGCAGUCUCCUUGGCUAUCCUUAUAUGUUCUUCUUUCUCAACUCAGAUAUUUACCUUCAUCCAAAAUAAGAUAGACAUGACAACAAAUAUAAUGGUGAGCGAAUGUUGACGAUUUUUGGAGGCU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAGCCAAGGAUGACUUGCCUG | miRNA | 43 | 63 | |
TTCTCTGAACAAAATGGTCGATGTC | primer | 5p | 65 | 89 |
GCCTCCAAAAATCGTCAACATTC | primer | 3p | 252 | 274 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169k found in PubMed |