Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.74799 | 0.308053 | 3.8769 | 0.453324 | |
| 10day-seedling | 3.20113 | 0.357708 | 5.00929 | 0.444843 | |
| 14day-seedling | 3.4967 | 0.384613 | 4.94834 | 0.762866 | |
| 25day-aerial | 3.79034 | 0.227228 | 5.52935 | 0.27396 | |
| 35day-aerial | 3.60168 | 0.162387 | 5.37504 | 0.296395 | |
| 42day-rosette | 2.63633 | 0.503321 | 4.48359 | 0.256927 | 42day-stem | 2.18714 | 0.272414 | 3.92293 | 0.399776 |
| 53day-rosette | 1.63027 | 0.851662 | 3.47277 | 0.784806 | 53day-stem | N/D | - | N/D | - | 53day-inflores | 1.1003 | 0.0493851 | 2.53518 | 0.150482 | 53day-silique | 3.32001 | 0.486842 | 4.4626 | 0.524654 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 35.38 | 11.53 | 3.39e-4 | -3.47 | 11.44 | 3.60e-4 | -3.44 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 34.35 | 10.08 | 9.24e-4 | -3.03 | 9.31 | 1.58e-3 | -2.8 | |
| 10day-seedling | 1 | 1.000 | 26.66 | 13.8 | 6.99e-5 | -4.16 | 8.31 | 3.15e-3 | -2.5 |
| 2 | 1.000 | 24.68 | 11.43 | 3.63e-4 | -3.44 | 5.62 | 2.04e-2 | -1.69 | |
| 3 | 1.000 | 24 | 12.63 | 1.58e-4 | -3.8 | 5.91 | 1.66e-2 | -1.78 | |
| 14day-seedling | 1 | 1.000 | 24.21 | 10.64 | 6.28e-4 | -3.2 | 3.99 | 6.28e-2 | -1.2 |
| 2 | 0.000 | 28.39 | 10.28 | 8.05e-4 | -3.09 | 7.37 | 6.03e-3 | -2.22 | |
| 3 | 0.996 | 28.9 | 11.11 | 4.51e-4 | -3.35 | 8.97 | 1.99e-3 | -2.7 | |
| 25day-aerial | 1 | 0.998 | 24.29 | 11.44 | 3.61e-4 | -3.44 | 5.68 | 1.96e-2 | -1.71 |
| 2 | 0.999 | 24.64 | 10.6 | 6.44e-4 | -3.19 | 4.36 | 4.88e-2 | -1.31 | |
| 3 | 1.000 | 23.81 | 9.93 | 1.03e-3 | -2.99 | 4.36 | 4.86e-2 | -1.31 | |
| 35day-aerial | 1 | 0.991 | 26.31 | 11.85 | 2.71e-4 | -3.57 | 6.31 | 1.26e-2 | -1.9 |
| 2 | 1.000 | 23.49 | 10.76 | 5.75e-4 | -3.24 | 4.63 | 4.04e-2 | -1.39 | |
| 3 | 0.999 | 24.43 | 11.24 | 4.13e-4 | -3.38 | 5.05 | 3.01e-2 | -1.52 | |
| 42day-rosette | 1 | 0.999 | 29.82 | 16.28 | 1.26e-5 | -4.9 | 9.18 | 1.73e-3 | -2.76 |
| 2 | 1.000 | 28.5 | 14.12 | 5.62e-5 | -4.25 | 8.43 | 2.91e-3 | -2.54 | |
| 3 | 0.999 | 26.56 | 13.04 | 1.19e-4 | -3.93 | 7.47 | 5.63e-3 | -2.25 | |
| 42day-stem | 1 | 0.999 | 28.72 | 17.32 | 7.46e-6 | -5.13 | 11.72 | 2.96e-1 | -3.53 |
| 2 | 0.999 | 27.84 | 15.3 | 2.49e-1 | -4.64 | 9.75 | 1.16e-1 | -2.93 | |
| 3 | 0.998 | 28.88 | 15.52 | 2.16e-1 | -4.67 | 9.19 | 1.77e-1 | -2.77 | |
| 53day-rosette | 1 | 1.000 | 28.78 | 14.73 | 3.67e-5 | -4.44 | 8.74 | 2.33e-3 | -2.63 |
| 2 | 1.000 | 30.8 | 18.51 | 2.68e-6 | -5.57 | 12.79 | 1.41e-4 | -3.85 | |
| 3 | 1.000 | 33.16 | 20.27 | 7.91e-7 | -6.1 | 13.61 | 7.98e-5 | -4.1 | |
| 53day-stem | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-inflores | 1 | 0.998 | 32.8 | 19.41 | 1.44e-6 | -5.84 | 14.26 | 5.11e-5 | -4.29 |
| 2 | 0.999 | 34.32 | 19.69 | 1.18e-6 | -5.93 | 15.14 | 2.77e-5 | -4.56 | |
| 3 | 0.999 | 33.52 | 19.69 | 1.18e-6 | -5.93 | 15.1 | 2.84e-5 | -4.55 | |
| 53day-silique | 1 | 1.000 | 30.28 | 10.43 | 7.24e-4 | -3.14 | 6.48 | 1.12e-2 | -1.95 |
| 2 | 1.000 | 31.96 | 12.67 | 1.54e-4 | -3.81 | 8.96 | 2.01e-3 | -2.7 | |
| 3 | 1.000 | 32.55 | 13.58 | 8.19e-5 | -4.09 | 9.84 | 1.09e-3 | -2.96 | |
NGS expression data
Expression of miR169j transcript in NGS dataSequences
miRNA transcript
GAGUAUAAUGAGGAAGAGAGGUCUAACAUGGCGAAAAGAGUCAUGUUUAGUAGCCAAGGAUGACUUGCCUGAUCUUUUUCACCUCCAUGAUUCAAUUUGUAAUUCAUGGGUUUUGGAUUAUUAUACAUUCAAAAGUAUAAUAAUUUGAAAUCAUGUUGAAUCUUGCGGGUUAGGUUUCAGGCAGUCUCCUUGGCUAUCUUGACAUGCUUUUUUCAUUCACG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAGCCAAGGAUGACUUGCCUG | miRNA | 51 | 71 | |
TAGTAGCCAAGGATGACTTGCCTGATC | primer | 5p | 48 | 74 |
AGCCAAGGAGACTGCCTGAAACC | primer | 3p | 173 | 195 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169j found in PubMed |