Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.0313 | 0.350355 | 3.1602 | 0.495626 | |
| 10day-seedling | 2.32394 | 0.347583 | 4.1321 | 0.455974 | |
| 14day-seedling | 1.8837 | 1.00182 | 2.78948 | 1.99162 | |
| 25day-aerial | 2.11394 | 1.0414 | 4.10755 | 1.15604 | |
| 35day-aerial | 2.33736 | 0.215965 | 3.93958 | 0.529199 | |
| 42day-rosette | 1.33832 | 0.442314 | 3.2288 | 0.474075 | 42day-stem | 1.37547 | 0.146076 | 3.1156 | 0.329321 |
| 53day-rosette | N/D | - | N/D | - | 53day-stem | 1.18912 | 1.22327 | 2.38654 | 0.141403 | 53day-inflores | -0.14586 | 0.028282 | 1.31627 | 0.149312 | 53day-silique | 2.13905 | 0.923874 | 3.28165 | 0.964432 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 37.86 | 14.01 | 6.08e-5 | -4.22 | 13.92 | 6.45e-5 | -4.19 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 36.63 | 12.36 | 1.90e-4 | -3.72 | 11.59 | 3.24e-4 | -3.49 | |
| 10day-seedling | 1 | 1.000 | 29.63 | 16.77 | 8.95e-6 | -5.05 | 11.28 | 4.03e-4 | -3.39 |
| 2 | 1.000 | 27.73 | 14.48 | 4.37e-5 | -4.36 | 8.67 | 2.45e-3 | -2.61 | |
| 3 | 1.000 | 26.72 | 15.35 | 2.40e-5 | -4.62 | 8.63 | 2.52e-3 | -2.6 | |
| 14day-seedling | 1 | 0.997 | 26.79 | 13.22 | 1.05e-4 | -3.98 | 6.58 | 1.05e-2 | -1.98 |
| 2 | 0.999 | 37.65 | 19.54 | 1.31e-6 | -5.88 | 16.64 | 9.81e-6 | -5.01 | |
| 3 | 0.989 | 35.99 | 18.21 | 3.31e-6 | -5.48 | 16.07 | 1.46e-5 | -4.84 | |
| 25day-aerial | 1 | 0.994 | 32.66 | 19.8 | 1.09e-6 | -5.96 | 14.04 | 5.93e-5 | -4.23 |
| 2 | 0.997 | 27.6 | 13.55 | 8.32e-5 | -4.08 | 7.31 | 6.30e-3 | -2.2 | |
| 3 | 0.999 | 26.81 | 12.93 | 1.28e-4 | -3.89 | 7.37 | 6.06e-3 | -2.22 | |
| 35day-aerial | 1 | 0.999 | 29.54 | 15.08 | 2.88e-5 | -4.54 | 9.55 | 1.34e-3 | -2.87 |
| 2 | 0.952 | 29.04 | 16.32 | 1.22e-5 | -4.91 | 10.18 | 8.59e-4 | -3.07 | |
| 3 | 0.999 | 28.15 | 14.96 | 3.14e-5 | -4.5 | 8.77 | 2.29e-3 | -2.64 | |
| 42day-rosette | 1 | 0.999 | 32.92 | 19.38 | 1.47e-6 | -5.83 | 12.27 | 2.02e-4 | -3.69 |
| 2 | 0.995 | 34.29 | 19.91 | 1.02e-6 | -5.99 | 14.21 | 5.26e-5 | -4.28 | |
| 3 | 0.999 | 30.19 | 16.66 | 9.64e-6 | -5.02 | 11.1 | 4.57e-4 | -3.34 | |
| 42day-stem | 1 | 0.999 | 30.83 | 19.14 | 1.73e-1 | -5.76 | 13.83 | 6.86e-1 | -4.16 |
| 2 | 0.999 | 31.27 | 18.73 | 2.30e-1 | -5.64 | 13.18 | 1.75e-1 | -3.97 | |
| 3 | 0.998 | 31.32 | 17.95 | 3.97e-6 | -5.47 | 11.63 | 3.15e-1 | -3.52 | |
| 53day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | 0.985 | 35.6 | 22.18 | 2.11e-7 | -6.68 | 15.66 | 1.93e-5 | -4.71 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.960 | 34.02 | 16.41 | 1.15e-5 | -4.94 | 13.46 | 8.88e-5 | -4.05 | |
| 53day-inflores | 1 | 0.999 | 37.05 | 23.66 | 7.56e-8 | -7.12 | 18.5 | 2.69e-6 | -5.57 |
| 2 | 1.000 | 38.47 | 23.84 | 6.66e-8 | -7.18 | 19.28 | 1.57e-6 | -5.81 | |
| 3 | 0.982 | 37.12 | 23.29 | 9.76e-8 | -7.01 | 18.7 | 2.35e-6 | -5.63 | |
| 53day-silique | 1 | 1.000 | 32.46 | 12.61 | 1.60e-4 | -3.8 | 8.66 | 2.47e-3 | -2.61 |
| 2 | 1.000 | 37.4 | 18.11 | 3.53e-6 | -5.45 | 14.41 | 4.60e-5 | -4.34 | |
| 3 | 1.000 | 36.69 | 17.72 | 4.63e-6 | -5.33 | 13.99 | 6.15e-5 | -4.21 | |
NGS expression data
Expression of miR169i transcript in NGS dataSequences
miRNA transcript
GAAGGAGAUGUCAAAGAUGAAUAGAAGAAUCAUAUUUGGUAGCCAAGGAUGACUUGCCUGACUCUUUGUGUAAAAUGUUUAGUGUCUUGUUUGAAGUCACUAUAAGUUGUAUCAAGCAAUGACCAUUUUGCUUAUAAAAAAGAUAUCAGGCAGUCUCCUUGGCUAUCCUUAUAUGUUCUUCUCUUUCAUCUCAGACAUUCACCUUCAUACAAAAUUAAAGAGGCACGACAUGAAUGAUCAUGGAAAGAGACAAUGUUGAUGACUUUGAGCUUUUUGCAUAUUGUCUAUAUAUAUGUAAUACAUUUUACGUUCAUGGAAGCUGUUUAAGACAAAUAUGUUCUAUAUCAGACAUAUAGACGUAAAUUUAGCUAAUGAUAACUGAAAUUCAAAUUUCUUUGACAUUGUUAGUGGCAACACUUGACGUGAAAG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UAGCCAAGGAUGACUUGCCUG | miRNA | 40 | 60 | |
GATGACTTTGAGCTTTTTGCATATTG | primer | 5p | 258 | 283 |
TTTCACGTCAAGTGTTGCCACTA | primer | 3p | 406 | 428 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169i found in PubMed |