Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.21517 | 0 | 3.44679 | 0 | |
| 10day-seedling | -0.01186 | 0.0536589 | 1.7963 | 0.140149 | |
| 14day-seedling | 0.61828 | 0 | 2.61844 | 0 | |
| 25day-aerial | N/D | - | N/D | - | |
| 35day-aerial | -0.22967 | 0 | 1.62612 | 0 | |
| 42day-rosette | N/D | - | N/D | - | 42day-stem | -0.52842 | 0.830238 | 1.10626 | 0.77908 |
| 53day-rosette | -0.40546 | 0.505367 | 1.43704 | 0.368663 | 53day-stem | 0.54721 | 0.750315 | 1.68872 | 0.0264294 | 53day-inflores | -0.58386 | 0 | 0.80141 | 0 | 53day-silique | 2.08507 | 0.47148 | 3.22767 | 0.504243 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 36.85 | 12.57 | 1.64e-4 | -3.78 | 11.8 | 2.80e-4 | -3.55 | |
| 10day-seedling | 1 | 1.000 | 35.97 | 23.11 | 1.10e-7 | -6.96 | 17.62 | 4.98e-6 | -5.3 |
| 2 | 1.000 | 36.55 | 23.3 | 9.67e-8 | -7.01 | 17.49 | 5.44e-6 | -5.26 | |
| 3 | 1.000 | 34.84 | 23.47 | 8.63e-8 | -7.06 | 16.75 | 9.05e-6 | -5.04 | |
| 14day-seedling | 1 | 1.000 | 34.77 | 21.2 | 4.15e-7 | -6.38 | 14.56 | 4.15e-5 | -4.38 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.998 | 36.71 | 23.98 | 6.02e-8 | -7.22 | 17.85 | 4.23e-6 | -5.37 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 42day-stem | 1 | 0.999 | 34.75 | 23.59 | 1.14e-7 | -6.94 | 17.75 | 4.54e-6 | -5.34 |
| 2 | 0.000 | 39.5 | 26.96 | 7.67e-1 | -8.12 | 21.49 | 3.59e-1 | -6.44 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | 1.000 | 38.77 | 24.72 | 3.62e-8 | -7.44 | 18.73 | 2.30e-6 | -5.64 |
| 2 | 1.000 | 35.15 | 22.86 | 1.31e-7 | -6.88 | 17.15 | 6.88e-6 | -5.16 | |
| 3 | 1.000 | 39.11 | 26.22 | 1.28e-8 | -7.89 | 19.56 | 1.29e-6 | -5.89 | |
| 53day-stem | 1 | 0.999 | 36.62 | 23.2 | 1.04e-7 | -6.98 | 16.68 | 9.53e-6 | -5.02 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 37.28 | 19.67 | 1.20e-6 | -5.92 | 16.72 | 9.27e-6 | -5.03 | |
| 53day-inflores | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 39.01 | 25.18 | 2.63e-8 | -7.58 | 20.59 | 6.33e-7 | -6.2 | |
| 53day-silique | 1 | 1.000 | 34.86 | 15.01 | 3.02e-5 | -4.52 | 11.06 | 4.67e-4 | -3.33 |
| 2 | 1.000 | 37.35 | 18.06 | 3.66e-6 | -5.44 | 14.36 | 4.77e-5 | -4.32 | |
| 3 | 1.000 | 34.88 | 15.91 | 1.63e-5 | -4.79 | 12.17 | 2.16e-4 | -3.66 | |
NGS expression data
Expression of miR169g transcript in NGS dataSequences
miRNA transcript
GAUGCGUCGAUGCCUUCAGAGUACGAAUAUUAUUCACAUAAAAUUCUUAUUCGAAUUUGAUAAUAUAAGGUCAGCCAAUCUUUUAAAGUAAUUAUAUUCUUCAAUAUACGGUUGUGGUCAAAAUUCCAUUUUAUUUUGUAGCUUGCAUGCACUACUAGUUUAAAACCAUGCAUGGAUUUAUUGCAUAUAAUAACAUUAUAUGAAUUUUCAAUUAAAUUAAUCCACACAUUUCCCAUUUCAAUAUGCCUAUAAAUACCUUCAUCACGAGUAUGACAAGAUCACAAGACAAGAAAAGAAAGGUAGAGAAAACAUGAUAAUGAUGAUUACGAUGAUGAGAGUCUCUAGUUGUAUCAGAGGGUCUUGCAUGGAAGAAUAGAGAAUGAGGUUGAGCCAAGGAUGACUUGCCGGGUUUUUUUACCAAUGAAUCUAAUUAACUGAUUCUGGUGUCCGGCAAGUUGACCUUGGCUCUGUUUCCUUCUCUUCUUUUGGAUGUCAGACUCCAAGAUAUCUAUCAUCAUGAAUCGUGAUCAAACUUUGUAAUUUCAUUGAAAUGUGUUUUUCUUGAUGCGAAUUUUUUGGCUUACGGUUUUUCGAUUUGAAUGAUCAGAUUUUUGUUUUUGCA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGAGCCAAGGAUGACUUGCCG | miRNA | 387 | 407 | |
ATGCGTCGATGCCTTCAGAG | primer | 5p | 2 | 21 |
TCTTGTCTTGTGATCTTGTCATACTCG | primer | 3p | 265 | 291 |
UCCGGCAAGUUGACCUUGGCU | miRNA* | * | 447 | 467 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169g found in PubMed |