Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.60165 | 0.834824 | 3.67416 | 0.976059 | |
| 10day-seedling | 1.87053 | 0.223748 | 3.67869 | 0.27048 | |
| 14day-seedling | 2.17253 | 0.566748 | 3.22251 | 1.24453 | |
| 25day-aerial | 4.60197 | 3.71164 | 6.073669 | 3.3076 | |
| 35day-aerial | 1.89126 | 0.234859 | 3.68481 | 0.29371 | |
| 42day-rosette | 1.16413 | 0 | 3.5266 | 0 | 42day-stem | 1.19097 | 0.552061 | 2.92355 | 0.487891 |
| 53day-rosette | 2.12444 | 0.676927 | 3.98676 | 0.476626 | 53day-stem | 3.81159 | 5.72306 | 5.22659 | 6.20144 | 53day-inflores | 1.18057 | 1.51255 | 2.6154 | 1.41349 | 53day-silique | 3.30209 | 0.621456 | 4.44468 | 0.660316 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 36.33 | 12.47 | 1.76e-4 | -3.75 | 12.39 | 1.87e-4 | -3.73 |
| 2 | 1.000 | 41.25 | 13.27 | 1.01e-4 | -4 | 13.41 | 9.20e-5 | -4.04 | |
| 3 | 1.000 | 32.39 | 8.12 | 3.59e-3 | -2.44 | 7.35 | 6.13e-3 | -2.21 | |
| 10day-seedling | 1 | 1.000 | 30.41 | 17.55 | 5.21e-6 | -5.28 | 12.06 | 2.35e-4 | -3.63 |
| 2 | 1.000 | 29.44 | 16.19 | 1.34e-5 | -4.87 | 10.37 | 7.54e-4 | -3.12 | |
| 3 | 1.000 | 28.75 | 17.38 | 5.86e-6 | -5.23 | 10.67 | 6.14e-4 | -3.21 | |
| 14day-seedling | 1 | 1.000 | 27.99 | 14.42 | 4.57e-5 | -4.34 | 7.77 | 4.57e-3 | -2.34 |
| 2 | 0.999 | 36.22 | 18.11 | 3.53e-6 | -5.45 | 15.21 | 2.65e-5 | -4.58 | |
| 3 | 0.997 | 33.35 | 15.56 | 2.07e-5 | -4.69 | 13.42 | 9.10e-5 | -4.04 | |
| 25day-aerial | 1 | 1.000 | 28.58 | 15.72 | 1.85e-5 | -4.73 | 9.96 | 1.00e-3 | -3 |
| 2 | 0.357 | 10.69 | -3.35 | 1.02e+1 | 1.01 | -9.59 | 7.73e+2 | 2.89 | |
| 3 | 1.000 | 28.31 | 14.43 | 4.55e-5 | -4.34 | 8.86 | 2.15e-3 | -2.67 | |
| 35day-aerial | 1 | 0.997 | 31.67 | 17.22 | 6.56e-6 | -5.18 | 11.68 | 3.05e-4 | -3.52 |
| 2 | 1.000 | 30.32 | 17.6 | 5.04e-6 | -5.3 | 11.47 | 3.54e-4 | -3.45 | |
| 3 | 1.000 | 29.27 | 16.08 | 1.44e-5 | -4.84 | 9.89 | 1.05e-3 | -2.98 | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 30.41 | 16.89 | 8.26e-6 | -5.08 | 11.32 | 3.92e-4 | -3.41 | |
| 42day-stem | 1 | 0.998 | 31.69 | 20 | 9.55e-7 | -6.2 | 14.69 | 3.79e-1 | -4.42 |
| 2 | 0.998 | 30.49 | 17.94 | 3.96e-1 | -5.42 | 12.4 | 1.86e-1 | -3.73 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 26.89 | 14.61 | 4.01e-5 | -4.4 | 8.89 | 2.11e-3 | -2.68 | |
| 3 | 1.000 | 30.68 | 17.79 | 4.42e-6 | -5.35 | 11.13 | 4.46e-4 | -3.35 | |
| 53day-stem | 1 | 0.000 | 2.09 | -11.33 | 2.58e+3 | 3.41 | -17.85 | 2.36e+5 | 5.37 |
| 2 | 0.999 | 35.89 | 21.12 | 4.39e-7 | -6.36 | 16.47 | 1.10e-5 | -4.96 | |
| 3 | 0.000 | 39.62 | 22.01 | 2.37e-7 | -6.63 | 19.06 | 1.83e-6 | -5.74 | |
| 53day-inflores | 1 | 0.983 | 38.47 | 25.08 | 2.82e-8 | -7.55 | 19.93 | 1.00e-6 | -6 |
| 2 | 1.000 | 31.77 | 17.15 | 6.90e-6 | -5.16 | 12.59 | 1.62e-4 | -3.79 | |
| 3 | 0.994 | 29.6 | 15.77 | 1.79e-5 | -4.75 | 11.18 | 4.31e-4 | -3.37 | |
| 53day-silique | 1 | 1.000 | 29.79 | 9.95 | 1.01e-3 | -2.99 | 6 | 1.57e-2 | -1.81 |
| 2 | 1.000 | 32.33 | 13.04 | 1.19e-4 | -3.93 | 9.34 | 1.55e-3 | -2.81 | |
| 3 | 1.000 | 32.83 | 13.86 | 6.71e-5 | -4.17 | 10.13 | 8.92e-4 | -3.05 | |
NGS expression data
Expression of miR169f transcript in NGS dataSequences
miRNA transcript
CACAAGAGUCACGAAAGAGGUAACACAUUUGAAAAUAAGUAGCUCGUGAUCGAAAGAAUAUUGUAAUGGGAGUCUUUGGUUGUAUCAGAGGGUCUUGCAUGAAGGAAUAACGAAUGGAAUUGAGCCAAGGAUGACUUGCCGGUUUAAACCCAACCGGUUUAUGACCAUUGAUUUGGUCUCAUUCACAAUCUGUUGAUUCGUGUCUGGCAAGUUGACCUUGGCUCUGCUUCGUUCUCUAUUCUUCCAUGUUAGAUUC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGAGCCAAGGAUGACUUGCCG | miRNA | 121 | 141 | |
ACAAGAGTCACGAAAGAGGTAACACAT | primer | 5p | 2 | 28 |
ATCAACAGATTGTGAATGAGACCAAA | primer | 3p | 172 | 197 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169f found in PubMed |