Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.51058 | 0.501843 | 3.58309 | 0.50139 | |
| 10day-seedling | 2.94834 | 0.202786 | 4.75649 | 0.208514 | |
| 14day-seedling | 4.10375 | 0.465281 | 5.16316 | 0.514958 | |
| 25day-aerial | 3.67423 | 0.352226 | 5.43915 | 0.265958 | |
| 35day-aerial | 3.3895 | 0.338909 | 5.16319 | 0.318406 | |
| 42day-rosette | 2.70613 | 0.597153 | 4.41766 | 0.170622 | 42day-stem | 2.31796 | 0.251449 | 3.71039 | 0.468165 |
| 53day-rosette | 2.12263 | 0.142043 | 3.96514 | 0.0342746 | 53day-stem | 2.7081 | 1.45991 | 3.85149 | 1.09612 | 53day-inflores | 2.17343 | 0.667773 | 3.60636 | 0.588051 | 53day-silique | 4.35823 | 0.21826 | 5.50082 | 0.247185 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 33.54 | 9.69 | 1.21e-3 | -2.92 | 9.6 | 1.29e-3 | -2.89 |
| 2 | 1.000 | 40.76 | 12.78 | 1.42e-4 | -3.85 | 12.91 | 1.30e-4 | -3.89 | |
| 3 | 1.000 | 36.58 | 12.31 | 1.97e-4 | -3.71 | 11.54 | 3.36e-4 | -3.47 | |
| 10day-seedling | 1 | 1.000 | 26.58 | 13.72 | 7.42e-5 | -4.13 | 8.22 | 3.35e-3 | -2.48 |
| 2 | 1.000 | 25.94 | 12.69 | 1.51e-4 | -3.82 | 6.88 | 8.48e-3 | -2.07 | |
| 3 | 1.000 | 25.33 | 13.97 | 6.25e-5 | -4.2 | 7.25 | 6.56e-3 | -2.18 | |
| 14day-seedling | 1 | 0.996 | 24.71 | 11.14 | 4.43e-4 | -3.35 | 4.5 | 4.43e-2 | -1.35 |
| 2 | 0.996 | 27.78 | 9.67 | 1.22e-3 | -2.91 | 6.77 | 9.17e-3 | -2.04 | |
| 3 | 0.988 | 25.84 | 8.05 | 3.78e-3 | -2.42 | 5.91 | 1.66e-2 | -1.78 | |
| 25day-aerial | 1 | 1.000 | 24.2 | 11.34 | 3.86e-4 | -3.41 | 5.58 | 2.09e-2 | -1.68 |
| 2 | 0.976 | 26.09 | 12.04 | 2.37e-4 | -3.63 | 5.8 | 1.79e-2 | -1.75 | |
| 3 | 1.000 | 23.62 | 9.74 | 1.17e-3 | -2.93 | 4.17 | 5.54e-2 | -1.26 | |
| 35day-aerial | 1 | 1.000 | 25.98 | 11.53 | 3.39e-4 | -3.47 | 5.99 | 1.57e-2 | -1.8 |
| 2 | 1.000 | 26 | 13.28 | 1.01e-4 | -4 | 7.15 | 7.06e-3 | -2.15 | |
| 3 | 0.998 | 24.37 | 11.18 | 4.32e-4 | -3.36 | 4.99 | 3.15e-2 | -1.5 | |
| 42day-rosette | 1 | 1.000 | 29.73 | 16.19 | 1.34e-5 | -4.87 | 9.08 | 1.85e-3 | -2.73 |
| 2 | 0.999 | 28.76 | 14.38 | 4.69e-5 | -4.33 | 8.69 | 2.42e-3 | -2.62 | |
| 3 | 1.000 | 25.75 | 12.22 | 2.09e-4 | -3.68 | 6.66 | 9.91e-3 | -2 | |
| 42day-stem | 1 | 1.000 | 27.19 | 15.51 | 2.16e-1 | -4.67 | 1.19 | 8.57e-1 | -3.67 |
| 2 | 1.000 | 27.29 | 14.75 | 3.64e-1 | -4.44 | 9.2 | 1.74e-1 | -2.77 | |
| 3 | 1.000 | 29.8 | 16.41 | 1.15e-1 | -4.94 | 1.18 | 9.67e-1 | -3.43 | |
| 53day-rosette | 1 | 1.000 | 30.25 | 16.2 | 1.33e-5 | -4.88 | 10.21 | 8.42e-4 | -3.07 |
| 2 | 1.000 | 28.02 | 15.73 | 1.84e-5 | -4.74 | 10.01 | 9.67e-4 | -3.01 | |
| 3 | 1.000 | 29.57 | 16.67 | 9.56e-6 | -5.02 | 10.02 | 9.65e-4 | -3.02 | |
| 53day-stem | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 32.46 | 17.69 | 4.74e-6 | -5.32 | 13.03 | 1.19e-4 | -3.92 | |
| 3 | 0.999 | 28.43 | 10.83 | 5.50e-4 | -3.26 | 7.87 | 4.26e-3 | -2.37 | |
| 53day-inflores | 1 | 1.000 | 31.6 | 18.21 | 3.30e-6 | -5.48 | 13.05 | 1.18e-4 | -3.93 |
| 2 | 1.000 | 30.75 | 16.12 | 1.41e-5 | -4.85 | 11.56 | 3.31e-4 | -3.48 | |
| 3 | 0.999 | 27.6 | 13.77 | 7.14e-5 | -4.15 | 9.18 | 1.72e-3 | -2.76 | |
| 53day-silique | 1 | 1.000 | 28 | 8.16 | 3.50e-3 | -2.46 | 4.21 | 5.42e-2 | -1.27 |
| 2 | 1.000 | 27.89 | 8.6 | 2.58e-3 | -2.59 | 4.89 | 3.37e-2 | -1.47 | |
| 3 | 1.000 | 28.54 | 9.57 | 1.31e-3 | -2.88 | 5.84 | 1.74e-2 | -1.76 | |
NGS expression data
Expression of miR169e transcript in NGS dataSequences
miRNA transcript
UGAUGAUGAUGAUGAGUCACUAAUUAAUUGUAUCAUAGAGUCUUGCAUGGAAAAAUAGAAAAUGAGAUUGAGCCAAGGAUGACUUGCCGAUUUUCUCAACGAAUCUUACUGAUUAUGGUAUCCGGCAAGUUGACUUUGGCUCUGUUUCCUUCCCUUCUUUUCGAUGUCAAACUCUAGAUACCUAACCACAUAUCAUAUAUAUCAUCAUCAUUCAUCAAUCAUCAUGAUUAGGGGUUGCUUUGUAGUAUCGUUGA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGAGCCAAGGAUGACUUGCCG | miRNA | 69 | 89 | |
CAAGTTGACTTTGGCTCTGTTTCC | primer | 5p | 126 | 149 |
CAACGATACTACAAAGCAACCCCTAAT | primer | 3p | 227 | 253 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169e found in PubMed |