Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.67944 | 0.629588 | 5.75196 | 0.497414 | |
| 10day-seedling | 1.85521 | 0.302234 | 3.66336 | 0.162064 | |
| 14day-seedling | 2.47614 | 1.22051 | 3.65396 | 0.525763 | |
| 25day-aerial | 1.93842 | 0.194461 | 3.73363 | 0.0834356 | |
| 35day-aerial | 2.02206 | 0.652103 | 3.61151 | 0.657118 | |
| 42day-rosette | 1.62904 | 0.845775 | 3.45259 | 0.617041 | 42day-stem | 2.14797 | 0.313335 | 3.94928 | 0.187203 |
| 53day-rosette | 2.46648 | 0.338464 | 4.30899 | 0.216032 | 53day-stem | 2.66866 | 0.952937 | 3.97781 | 0.670235 | 53day-inflores | 2.08606 | 0.656276 | 3.69703 | 0.759123 | 53day-silique | 4.33994 | 0.0302972 | 5.48254 | 0.0389343 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 28.34 | 4.48 | 4.47e-2 | -1.35 | 4.4 | 4.75e-2 | -1.32 |
| 2 | 1.000 | 30.23 | 2.25 | 2.10e-1 | -0.68 | 2.38 | 1.92e-1 | -0.72 | |
| 3 | 1.000 | 30.7 | 6.43 | 1.16e-2 | -1.94 | 5.66 | 1.98e-2 | -1.7 | |
| 10day-seedling | 1 | 1.000 | 28.84 | 15.98 | 1.54e-5 | -4.81 | 10.49 | 6.96e-4 | -3.16 |
| 2 | 1.000 | 30.6 | 17.35 | 5.99e-6 | -5.22 | 11.54 | 3.37e-4 | -3.47 | |
| 3 | 1.000 | 29.31 | 17.94 | 3.98e-6 | -5.4 | 11.23 | 4.17e-4 | -3.38 | |
| 14day-seedling | 1 | 1.000 | 30.48 | 16.91 | 8.12e-6 | -5.09 | 10.27 | 8.13e-4 | -3.09 |
| 2 | 0.000 | 30.58 | 12.47 | 1.76e-4 | -3.75 | 9.57 | 1.32e-3 | -2.88 | |
| 3 | 1.000 | 30.7 | 12.91 | 1.30e-4 | -3.89 | 10.77 | 5.72e-4 | -3.24 | |
| 25day-aerial | 1 | 0.997 | 29.51 | 16.65 | 9.73e-6 | -5.01 | 10.89 | 5.27e-4 | -3.28 |
| 2 | 0.997 | 31.39 | 17.35 | 6.00e-6 | -5.22 | 11.1 | 4.54e-4 | -3.34 | |
| 3 | 0.999 | 29.98 | 16.1 | 1.43e-5 | -4.85 | 10.53 | 6.75e-4 | -3.17 | |
| 35day-aerial | 1 | 0.995 | 30.18 | 15.73 | 1.84e-5 | -4.73 | 10.19 | 8.55e-4 | -3.07 |
| 2 | 0.999 | 27.61 | 14.89 | 3.30e-5 | -4.48 | 8.76 | 2.31e-3 | -2.64 | |
| 3 | 0.998 | 32.16 | 18.97 | 1.95e-6 | -5.71 | 12.78 | 1.42e-4 | -3.85 | |
| 42day-rosette | 1 | 0.999 | 34.45 | 20.91 | 5.07e-7 | -6.3 | 13.81 | 6.98e-5 | -4.16 |
| 2 | 0.999 | 29.78 | 15.4 | 2.32e-5 | -4.64 | 9.71 | 1.20e-3 | -2.92 | |
| 3 | 0.999 | 30.74 | 17.22 | 6.57e-6 | -5.18 | 11.65 | 3.12e-4 | -3.51 | |
| 42day-stem | 1 | 0.998 | 27.59 | 15.9 | 1.64e-1 | -4.79 | 1.59 | 6.57e-1 | -3.19 |
| 2 | 0.999 | 27.52 | 14.97 | 3.17e-1 | -4.58 | 9.43 | 1.45e-1 | -2.84 | |
| 3 | 1.000 | 30.06 | 16.67 | 9.56e-6 | -5.19 | 1.37 | 7.57e-1 | -3.13 | |
| 53day-rosette | 1 | 1.000 | 28.29 | 14.24 | 5.16e-5 | -4.29 | 8.25 | 3.28e-3 | -2.48 |
| 2 | 1.000 | 26.88 | 14.59 | 4.04e-5 | -4.39 | 8.88 | 2.12e-3 | -2.67 | |
| 3 | 1.000 | 29.23 | 16.34 | 1.20e-5 | -4.92 | 9.69 | 1.21e-3 | -2.92 | |
| 53day-stem | 1 | 0.998 | 26.9 | 13.48 | 8.75e-5 | -4.06 | 6.96 | 8.02e-3 | -2.1 |
| 2 | 1.000 | 31.73 | 16.95 | 7.89e-6 | -5.1 | 12.3 | 1.98e-4 | -3.7 | |
| 3 | 1.000 | 28.49 | 10.88 | 5.29e-4 | -3.28 | 7.93 | 4.10e-3 | -2.39 | |
| 53day-inflores | 1 | 0.998 | 32.23 | 18.84 | 2.13e-6 | -5.67 | 13.69 | 7.58e-5 | -4.12 |
| 2 | 0.999 | 29.69 | 15.06 | 2.92e-5 | -4.53 | 10.51 | 6.87e-4 | -3.16 | |
| 3 | 0.988 | 27.13 | 13.31 | 9.88e-5 | -4.01 | 8.72 | 2.38e-3 | -2.62 | |
| 53day-silique | 1 | 1.000 | 28.74 | 8.9 | 2.10e-3 | -2.68 | 4.95 | 3.24e-2 | -1.49 |
| 2 | 1.000 | 28.18 | 8.89 | 2.10e-3 | -2.68 | 5.19 | 2.74e-2 | -1.56 | |
| 3 | 1.000 | 27.69 | 8.72 | 2.37e-3 | -2.63 | 4.99 | 3.15e-2 | -1.5 | |
NGS expression data
Expression of miR169d transcript in NGS dataSequences
miRNA transcript
UUCAUCAUGAGUUAGGGUUUUGCCAUAACAAUAUCACAAGAAAAUAUGAGAGGUGCAAAAAAAAACAAGAUGAUAAUGAGAGUCACUAAUUGUAUCAUAGAGUCUUGCAUGGAAAAAUUAAAGAAUGAGAUUGAGCCAAGGAUGACUUGCCGAUGUUAUCAACAAAUCUUAACUGAUUUUGGUGUCCGGCAAGUUGACCUUGGCUCUGUUUCCUUCUUUUCUUUUCAAUGUCAAACUCUAGAUAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGAGCCAAGGAUGACUUGCCG | miRNA | 132 | 152 | |
TCATCATGAGTTAGGGTTTTGC | primer | 5p | 2 | 23 |
AGGAAACAGAGCCAAGGTCA | primer | 3p | 195 | 214 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169d found in PubMed |