Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.1088 | 0.590939 | 5.18132 | 0.458209 | |
| 10day-seedling | 1.60436 | 0.430361 | 3.41251 | 0.279354 | |
| 14day-seedling | 1.86067 | 0.633579 | 2.8196 | 1.17335 | |
| 25day-aerial | 1.56801 | 0.0676036 | 3.27893 | 0.105392 | |
| 35day-aerial | 1.50226 | 0.477995 | 3.20634 | 0.942007 | |
| 42day-rosette | 1.33811 | 1.32344 | 3.22071 | 1.39013 | 42day-stem | 1.96368 | 0.231374 | 3.6836 | 0.385498 |
| 53day-rosette | 1.74716 | 0.0165439 | 3.50894 | 0.0415669 | 53day-stem | 2.62662 | 1.34542 | 4.04388 | 0.921748 | 53day-inflores | 2.65133 | 0.412767 | 3.80701 | 0.726308 | 53day-silique | 3.48169 | 0.646977 | 4.62429 | 0.686065 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 28.86 | 5 | 3.12e-2 | -1.51 | 4.91 | 3.32e-2 | -1.48 |
| 2 | 1.000 | 33.28 | 5.3 | 2.53e-2 | -1.6 | 5.44 | 2.31e-2 | -1.64 | |
| 3 | 1.000 | 32.81 | 8.54 | 2.68e-3 | -2.57 | 7.77 | 4.57e-3 | -2.34 | |
| 10day-seedling | 1 | 1.000 | 29.2 | 16.34 | 1.20e-5 | -4.92 | 10.85 | 5.43e-4 | -3.27 |
| 2 | 1.000 | 31.56 | 18.31 | 3.08e-6 | -5.51 | 12.5 | 1.73e-4 | -3.76 | |
| 3 | 1.000 | 30.49 | 19.12 | 1.75e-6 | -5.76 | 12.41 | 1.84e-4 | -3.74 | |
| 14day-seedling | 1 | 0.988 | 29.63 | 16.06 | 1.46e-5 | -4.84 | 9.42 | 1.46e-3 | -2.84 |
| 2 | 0.997 | 37.6 | 19.49 | 1.36e-6 | -5.87 | 16.59 | 1.02e-5 | -4.99 | |
| 3 | 0.993 | 33.45 | 15.66 | 1.93e-5 | -4.71 | 13.52 | 8.49e-5 | -4.07 | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.997 | 32.25 | 18.2 | 3.31e-6 | -5.48 | 11.96 | 2.51e-4 | -3.6 | |
| 3 | 0.997 | 31.77 | 17.89 | 4.13e-6 | -5.38 | 12.32 | 1.95e-4 | -3.71 | |
| 35day-aerial | 1 | 0.996 | 31.28 | 16.83 | 8.59e-6 | -5.07 | 11.29 | 3.99e-4 | -3.4 |
| 2 | 0.999 | 29.2 | 16.47 | 1.10e-5 | -4.96 | 10.34 | 7.71e-4 | -3.11 | |
| 3 | 0.998 | 32.7 | 19.5 | 1.35e-6 | -5.87 | 13.32 | 9.81e-5 | -4.01 | |
| 42day-rosette | 1 | 0.997 | 29.72 | 16.19 | 1.34e-5 | -4.87 | 9.08 | 1.85e-3 | -2.73 |
| 2 | 0.998 | 37.7 | 23.32 | 9.57e-8 | -7.02 | 17.62 | 4.95e-6 | -5.31 | |
| 3 | 1.000 | 29.88 | 16.36 | 1.19e-5 | -4.92 | 10.79 | 5.65e-4 | -3.25 | |
| 42day-stem | 1 | 0.985 | 28.65 | 16.95 | 7.87e-6 | -5.14 | 11.64 | 3.12e-1 | -3.55 |
| 2 | 0.996 | 29.79 | 17.24 | 6.44e-1 | -5.2 | 11.69 | 3.17e-1 | -3.52 | |
| 3 | 0.998 | 29.23 | 15.85 | 1.70e-1 | -4.77 | 9.54 | 1.34e-1 | -2.87 | |
| 53day-rosette | 1 | 1.000 | 31.54 | 17.49 | 5.44e-6 | -5.26 | 11.5 | 3.45e-4 | -3.46 |
| 2 | 1.000 | 29.7 | 17.41 | 5.74e-6 | -5.24 | 11.69 | 3.02e-4 | -3.52 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | 0.999 | 30.57 | 17.14 | 6.91e-6 | -5.16 | 10.62 | 6.33e-4 | -3.2 |
| 2 | 1.000 | 31.83 | 17.05 | 7.36e-6 | -5.13 | 12.4 | 1.85e-4 | -3.73 | |
| 3 | 1.000 | 26.97 | 9.37 | 1.51e-3 | -2.82 | 6.41 | 1.17e-2 | -1.93 | |
| 53day-inflores | 1 | 0.993 | 28.82 | 15.43 | 2.26e-5 | -4.65 | 10.28 | 8.05e-4 | -3.09 |
| 2 | 0.996 | 29.59 | 14.96 | 3.15e-5 | -4.5 | 10.4 | 7.40e-4 | -3.13 | |
| 3 | 0.996 | 26.71 | 12.88 | 1.33e-4 | -3.88 | 8.29 | 3.19e-3 | -2.5 | |
| 53day-silique | 1 | 1.000 | 29.09 | 9.25 | 1.65e-3 | -2.78 | 5.29 | 2.55e-2 | -1.59 |
| 2 | 1.000 | 31.82 | 12.53 | 1.70e-4 | -3.77 | 8.82 | 2.21e-3 | -2.66 | |
| 3 | 1.000 | 32.26 | 13.29 | 9.98e-5 | -4 | 9.56 | 1.33e-3 | -2.88 | |
NGS expression data
Expression of miR169c transcript in NGS dataSequences
miRNA transcript
UCGUCCAUUAUGAGUAUUAAUUAUGGUUAGGGAAUCUUACAGAAUGAAAAUGAAGGUGUGAAUGGAUUGUCUCAUCUAAAGCCUUGAAUGUGGGAAAAAGGCCAUUGUUGUUCAGCCAAGGAUGACUUGCCGGUAGCUUGUAUUAUGAUUACUCUAUAUUCGAUUUAUAUUAUGGAGAUGAUGGUUUAUAUAUAUUUACUUAUCUACAUAGUUUUAGUUGAUUUUUUUUCGUACGUAAUAUAAUACGAAAAAGUAUUUACUUAUUUAUAUAUGUGUGUUGGGGCAAGAAGUGUAACCAAGCUAGCCCGGCAAGUCAUCUAUGGCUAUGCAACUGUCUCUUCCUCUCAUUCUAGGCUUACGAUGACACGUAAAAAAUCCCAAAUAUCACUAAUAUGAUAUGAAUAUGGAUGA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CAGCCAAGGAUGACUUGCCGG | miRNA | 113 | 133 | |
TTCGATTTATATTATGGAGATGATGG | primer | 5p | 159 | 184 |
TTCTTGCCCCAACACACATA | primer | 3p | 270 | 289 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169c found in PubMed |