Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 1.73863 | 1.03104 | 1.83431 | 0.838784 | |
| 10day-seedling | 2.74737 | 0.114357 | 4.55552 | 0.294425 | |
| 14day-seedling | 3.18777 | 0.414377 | 4.08578 | 0.841567 | |
| 25day-aerial | 1.94394 | 0.150344 | 3.71747 | 0.178307 | |
| 35day-aerial | 1.62453 | 0.197214 | 3.41305 | 0.284253 | |
| 42day-rosette | 0.60418 | 1.05996 | 2.80243 | 1.79857 | 42day-stem | 1.41443 | 0.422565 | 3.37213 | 0.485285 |
| 53day-rosette | 0.92808 | 0 | 2.64877 | 0 | 53day-stem | 1.63221 | 0.132652 | 3.05812 | 0.8915 | 53day-inflores | 2.11122 | 0.212203 | 3.54518 | 0.116112 | 53day-silique | 3.40529 | 0 | 4.59451 | 0 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 43.03 | 15.06 | 2.94e-5 | -4.53 | 15.19 | 2.68e-5 | -4.57 | |
| 3 | 1.000 | 44.17 | 19.9 | 1.02e-6 | -5.99 | 19.13 | 1.74e-6 | -5.76 | |
| 10day-seedling | 1 | 1.000 | 27.09 | 14.23 | 5.20e-5 | -4.28 | 8.74 | 2.34e-3 | -2.63 |
| 2 | 1.000 | 27.69 | 14.44 | 4.49e-5 | -4.35 | 8.63 | 2.52e-3 | -2.6 | |
| 3 | 1.000 | 25.07 | 13.71 | 7.49e-5 | -4.13 | 6.99 | 7.85e-3 | -2.11 | |
| 14day-seedling | 1 | 0.999 | 27.46 | 13.89 | 6.60e-5 | -4.18 | 7.24 | 6.60e-3 | -2.18 |
| 2 | 0.981 | 31.01 | 12.9 | 1.31e-4 | -3.88 | 9.99 | 9.81e-4 | -3.01 | |
| 3 | 0.999 | 28.97 | 11.19 | 4.29e-4 | -3.37 | 9.05 | 1.89e-3 | -2.72 | |
| 25day-aerial | 1 | 1.000 | 30.14 | 17.28 | 6.28e-6 | -5.2 | 11.52 | 3.40e-4 | -3.47 |
| 2 | 1.000 | 30.63 | 16.58 | 1.02e-5 | -4.99 | 10.34 | 7.71e-4 | -3.11 | |
| 3 | 1.000 | 30.29 | 16.41 | 1.15e-5 | -4.94 | 10.85 | 5.42e-4 | -3.27 | |
| 35day-aerial | 1 | 0.999 | 32.31 | 17.86 | 4.21e-6 | -5.38 | 12.32 | 1.96e-4 | -3.71 |
| 2 | 0.999 | 29.69 | 16.97 | 7.80e-6 | -5.11 | 10.83 | 5.48e-4 | -3.26 | |
| 3 | 0.999 | 31.7 | 18.51 | 2.68e-6 | -5.57 | 12.32 | 1.95e-4 | -3.71 | |
| 42day-rosette | 1 | 1.000 | 30.36 | 16.82 | 8.62e-6 | -5.06 | 9.72 | 1.19e-3 | -2.93 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.999 | 37.26 | 23.74 | 7.16e-8 | -7.15 | 18.17 | 3.39e-6 | -5.47 | |
| 42day-stem | 1 | 0.995 | 28.61 | 16.92 | 8.54e-1 | -5.95 | 11.61 | 3.20e-1 | -3.5 |
| 2 | 0.999 | 31.43 | 18.89 | 2.56e-1 | -5.69 | 13.34 | 9.63e-1 | -4.17 | |
| 3 | 1.000 | 30.39 | 17.12 | 7.57e-6 | -5.12 | 1.75 | 5.99e-1 | -3.22 | |
| 53day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 32.46 | 20.17 | 8.47e-7 | -6.07 | 14.45 | 4.45e-5 | -4.35 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | 0.998 | 30.94 | 17.52 | 5.32e-6 | -5.27 | 11 | 4.88e-4 | -3.31 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 35.75 | 18.14 | 3.46e-6 | -5.46 | 15.19 | 2.68e-5 | -4.57 | |
| 53day-inflores | 1 | 0.999 | 30.4 | 17.01 | 7.58e-6 | -5.12 | 11.86 | 2.70e-4 | -3.57 |
| 2 | 0.999 | 30.67 | 16.04 | 1.48e-5 | -4.83 | 11.49 | 3.48e-4 | -3.46 | |
| 3 | 0.998 | 29.48 | 15.65 | 1.94e-5 | -4.71 | 11.06 | 4.67e-4 | -3.33 | |
| 53day-silique | 1 | 1.000 | 31.79 | 11.94 | 2.54e-4 | -3.59 | 7.99 | 3.93e-3 | -2.41 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
NGS expression data
Expression of miR169b transcript in NGS dataSequences
miRNA transcript
GCCCAACGGAGUAGAAUUGCAUGAAGUGGAGUAGAGUAUAAUGCAGCCAAGGAUGACUUGCCGGAACGUUGUUAACCAUGCAUAUGAAUAAUGUGAUGAUUAAUUAUGUGAUGAACAUAUUUCUGGCAAGUUGUCCUUCGGCUACAUUUUGCUCUCUUCUUCUCAUGCAAACUUUCCUUGGG
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CAGCCAAGGAUGACUUGCCGG | miRNA | 44 | 64 | |
CCCAACGGAGTAGAATTGCATGAAGTG | primer | 5p | 2 | 28 |
GCCGAAGGACAACTTGCCAGAAATATG | primer | 3p | 116 | 142 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169b found in PubMed |