Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.91145 | 0.246925 | 4.98396 | 0.353645 | |
| 10day-seedling | 3.88672 | 0.316969 | 5.69488 | 0.144016 | |
| 14day-seedling | 4.87618 | 0.474226 | 6.04888 | 0.265664 | |
| 25day-aerial | 3.99831 | 0.160796 | 5.55022 | 0.266573 | |
| 35day-aerial | 3.85528 | 0.593716 | 5.9547 | 0.216796 | |
| 42day-rosette | 3.49058 | 0.223542 | 5.20762 | 0.620225 | 42day-stem | 3.62204 | 0.543066 | 5.33839 | 0.665075 |
| 53day-rosette | 2.72652 | 0.212613 | 4.56902 | 0.199481 | 53day-stem | 2.91418 | 0.670387 | 4.47262 | 0.343772 | 53day-inflores | 2.91236 | 0.318448 | 4.44453 | 0.18245 | 53day-silique | 4.18382 | 0.176698 | 5.32641 | 0.209337 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 30.26 | 6.4 | 1.18e-2 | -1.93 | 6.31 | 1.26e-2 | -1.9 |
| 2 | 1.000 | 35.86 | 7.88 | 4.24e-3 | -2.37 | 8.02 | 3.86e-3 | -2.41 | |
| 3 | 1.000 | 30.8 | 6.53 | 1.08e-2 | -1.97 | 5.76 | 1.84e-2 | -1.73 | |
| 10day-seedling | 1 | 1.000 | 22.14 | 9.28 | 1.61e-3 | -2.79 | 3.79 | 7.24e-2 | -1.14 |
| 2 | 1.000 | 23.6 | 10.36 | 7.63e-4 | -3.12 | 4.54 | 4.29e-2 | -1.37 | |
| 3 | 1.000 | 22.76 | 11.39 | 3.73e-4 | -3.43 | 4.68 | 3.91e-2 | -1.41 | |
| 14day-seedling | 1 | 0.997 | 22.45 | 8.87 | 2.13e-3 | -2.67 | 2.23 | 2.13e-1 | -0.67 |
| 2 | 0.000 | 24.28 | 6.17 | 1.39e-2 | -1.86 | 3.26 | 1.04e-1 | -0.98 | |
| 3 | 0.999 | 23.91 | 6.12 | 1.43e-2 | -1.84 | 3.99 | 6.31e-2 | -1.2 | |
| 25day-aerial | 1 | 0.999 | 23.18 | 10.33 | 7.79e-4 | -3.11 | 4.57 | 4.22e-2 | -1.37 |
| 2 | 1.000 | 23.63 | 9.59 | 1.30e-3 | -2.89 | 3.35 | 9.83e-2 | -1.01 | |
| 3 | 1.000 | 23.52 | 9.64 | 1.25e-3 | -2.9 | 4.08 | 5.91e-2 | -1.23 | |
| 35day-aerial | 1 | 0.998 | 22.64 | 8.18 | 3.44e-3 | -2.46 | 2.65 | 1.60e-1 | -0.8 |
| 2 | 0.999 | 22.83 | 10.1 | 9.08e-4 | -3.04 | 3.97 | 6.37e-2 | -1.2 | |
| 3 | 0.930 | 23.18 | 9.98 | 9.87e-4 | -3.01 | 3.8 | 7.20e-2 | -1.14 | |
| 42day-rosette | 1 | 0.999 | 24.56 | 11.02 | 4.81e-4 | -3.32 | 3.92 | 6.62e-2 | -1.18 |
| 2 | 0.999 | 26.86 | 12.47 | 1.76e-4 | -3.76 | 6.78 | 9.08e-3 | -2.04 | |
| 3 | 0.999 | 25 | 11.48 | 3.51e-4 | -3.45 | 5.91 | 1.67e-2 | -1.78 | |
| 42day-stem | 1 | 0.998 | 23.05 | 11.36 | 3.89e-1 | -3.42 | 6.49 | 1.51e-1 | -1.83 |
| 2 | 1.000 | 25.5 | 12.95 | 1.26e-1 | -3.9 | 7.43 | 5.97e-1 | -2.23 | |
| 3 | 0.999 | 22.69 | 9.37 | 1.58e-1 | -2.82 | 3 | 1.25e-1 | -0.93 | |
| 53day-rosette | 1 | 1.000 | 27.45 | 13.4 | 9.26e-5 | -4.03 | 7.41 | 5.88e-3 | -2.23 |
| 2 | 1.000 | 26.74 | 14.45 | 4.47e-5 | -4.35 | 8.73 | 2.35e-3 | -2.63 | |
| 3 | 1.000 | 27.63 | 14.74 | 3.65e-5 | -4.44 | 8.08 | 3.69e-3 | -2.43 | |
| 53day-stem | 1 | 0.999 | 29.37 | 15.95 | 1.59e-5 | -4.8 | 9.43 | 1.45e-3 | -2.84 |
| 2 | 1.000 | 28.02 | 13.25 | 1.03e-4 | -3.99 | 8.59 | 2.59e-3 | -2.59 | |
| 3 | 0.999 | 27.73 | 10.12 | 8.97e-4 | -3.05 | 7.17 | 6.95e-3 | -2.16 | |
| 53day-inflores | 1 | 1.000 | 27.38 | 13.99 | 6.14e-5 | -4.21 | 8.84 | 2.19e-3 | -2.66 |
| 2 | 1.000 | 28.02 | 13.4 | 9.28e-5 | -4.03 | 8.84 | 2.18e-3 | -2.66 | |
| 3 | 1.000 | 26.21 | 12.38 | 1.88e-4 | -3.73 | 7.79 | 4.53e-3 | -2.34 | |
| 53day-silique | 1 | 1.000 | 28.64 | 8.8 | 2.25e-3 | -2.65 | 4.85 | 3.48e-2 | -1.46 |
| 2 | 1.000 | 28.6 | 9.3 | 1.58e-3 | -2.8 | 5.6 | 2.06e-2 | -1.69 | |
| 3 | 1.000 | 28.94 | 9.97 | 1.00e-3 | -3 | 6.23 | 1.33e-2 | -1.88 | |
NGS expression data
Expression of miR169a transcript in NGS dataSequences
miRNA transcript
GUGACGAAAGUAGUGUGCAGCCAAGGAUGACUUGCCGAUUUAAAUGAUCUUUCUUUAUACUCUAUUAAGACAAUUUAGUUUCAAACUUUUUUUUUUUUUUUUUUUUGAAGGAUUCAGGAAGAAAUUAGGAUAUAUUAUUCCGUAUAAAAUACAAGAUAUAUAAAACCAAAAAGAAAAAGUAACAUGAUCGGCAAGUUGUCCUUGGCUACACGUUACUUUGUGUCGCCACGUUACUUUGUGUCGCGCAUAGGUGUUAUAUGGUAAAGAUCGCAAGAACUCAAGCUAAGGAACAAUGCAGAAAGAGGUAACUAAUU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
CAGCCAAGGAUGACUUGCCGA | miRNA | 18 | 38 | |
AAAGTAACATGATCGGCAAGTTGTC | primer | 5p | 176 | 200 |
ATTAGTTACCTCTTTCTGCATTGTTCCT | primer | 3p | 286 | 313 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR169a found in PubMed |