Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.21624 | 0.986376 | 4.28876 | 1.12574 | |
| 10day-seedling | 3.13961 | 0.457527 | 4.94777 | 0.269708 | |
| 14day-seedling | 3.76408 | 0.601123 | 4.77537 | 0.329364 | |
| 25day-aerial | 3.22714 | 0.305147 | 4.98967 | 0.200562 | |
| 35day-aerial | 3.53823 | 0.124128 | 5.32362 | 0.0535236 | |
| 42day-rosette | 3.70617 | 0.47825 | 5.49259 | 0.124504 | 42day-stem | 3.37284 | 0.044189 | 4.89013 | 0.379134 |
| 53day-rosette | 4.22405 | 0.224691 | 6.066552 | 0.188851 | 53day-stem | 4.20547 | 0.874155 | 5.62371 | 0.363899 | 53day-inflores | 3.27994 | 0.237419 | 4.72604 | 0.157658 | 53day-silique | 3.91083 | 0.372701 | 5.05342 | 0.412009 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.86 | 11.01 | 4.86e-4 | -3.31 | 10.92 | 5.16e-4 | -3.29 |
| 2 | 1.000 | 39.25 | 11.27 | 4.05e-4 | -3.39 | 11.4 | 3.69e-4 | -3.43 | |
| 3 | 1.000 | 29.74 | 5.47 | 2.26e-2 | -1.65 | 4.7 | 3.85e-2 | -1.41 | |
| 10day-seedling | 1 | 1.000 | 24.31 | 11.45 | 3.58e-4 | -3.45 | 5.95 | 1.61e-2 | -1.79 |
| 2 | 1.000 | 25.82 | 12.57 | 1.65e-4 | -3.78 | 6.76 | 9.25e-3 | -2.03 | |
| 3 | 1.000 | 25.82 | 14.46 | 4.45e-5 | -4.35 | 7.74 | 4.67e-3 | -2.33 | |
| 14day-seedling | 1 | 0.999 | 26.48 | 12.91 | 1.30e-4 | -3.88 | 6.26 | 1.30e-2 | -1.88 |
| 2 | 0.999 | 28.48 | 10.37 | 7.58e-4 | -3.12 | 7.46 | 5.67e-3 | -2.25 | |
| 3 | 1.000 | 26.75 | 8.96 | 2.00e-3 | -2.7 | 6.82 | 8.83e-3 | -2.05 | |
| 25day-aerial | 1 | 0.998 | 25 | 12.14 | 2.21e-4 | -3.66 | 6.39 | 1.20e-2 | -1.92 |
| 2 | 0.998 | 27.73 | 13.68 | 7.60e-5 | -4.12 | 7.44 | 5.76e-3 | -2.24 | |
| 3 | 0.999 | 25.65 | 11.77 | 2.86e-4 | -3.54 | 6.21 | 1.35e-2 | -1.87 | |
| 35day-aerial | 1 | 0.999 | 25.42 | 10.96 | 5.01e-4 | -3.3 | 5.43 | 2.33e-2 | -1.63 |
| 2 | 0.999 | 24.62 | 11.9 | 2.62e-4 | -3.58 | 5.77 | 1.84e-2 | -1.74 | |
| 3 | 0.999 | 24.71 | 11.52 | 3.40e-4 | -3.47 | 5.33 | 2.48e-2 | -1.61 | |
| 42day-rosette | 1 | 0.998 | 25.87 | 12.33 | 1.94e-4 | -3.71 | 5.22 | 2.68e-2 | -1.57 |
| 2 | 0.999 | 23.59 | 9.21 | 1.69e-3 | -2.77 | 3.52 | 8.74e-2 | -1.06 | |
| 3 | 1.000 | 23.62 | 10.1 | 9.12e-4 | -3.04 | 4.53 | 4.33e-2 | -1.36 | |
| 42day-stem | 1 | 1.000 | 23.72 | 12.24 | 2.41e-1 | -3.62 | 6.71 | 9.53e-1 | -2.22 |
| 2 | 1.000 | 24.75 | 12.27 | 2.12e-1 | -3.67 | 6.66 | 9.93e-1 | -2.42 | |
| 3 | 1.000 | 25.3 | 11.92 | 2.59e-1 | -3.59 | 5.7 | 2.48e-1 | -1.69 | |
| 53day-rosette | 1 | 1.000 | 22.46 | 8.41 | 2.94e-3 | -2.53 | 2.42 | 1.87e-1 | -0.73 |
| 2 | 1.000 | 21.66 | 9.37 | 1.51e-3 | -2.82 | 3.66 | 7.92e-2 | -1.1 | |
| 3 | 1.000 | 22.77 | 9.88 | 1.06e-3 | -2.97 | 3.22 | 1.07e-1 | -0.97 | |
| 53day-stem | 1 | 1.000 | 25.25 | 11.83 | 2.75e-4 | -3.56 | 5.31 | 2.52e-2 | -1.6 |
| 2 | 0.999 | 24.64 | 9.87 | 1.07e-3 | -2.97 | 5.22 | 2.69e-2 | -1.57 | |
| 3 | 0.999 | 23.74 | 6.13 | 1.43e-2 | -1.85 | 3.18 | 1.11e-1 | -0.96 | |
| 53day-inflores | 1 | 0.999 | 26.64 | 13.25 | 1.03e-4 | -3.99 | 8.1 | 3.66e-3 | -2.44 |
| 2 | 1.000 | 26.7 | 12.07 | 2.32e-4 | -3.63 | 7.52 | 5.46e-3 | -2.26 | |
| 3 | 0.998 | 25.47 | 11.64 | 3.14e-4 | -3.5 | 7.05 | 7.55e-3 | -2.12 | |
| 53day-silique | 1 | 1.000 | 28.77 | 8.93 | 2.05e-3 | -2.69 | 4.98 | 3.18e-2 | -1.5 |
| 2 | 1.000 | 30.67 | 11.37 | 3.77e-4 | -3.42 | 7.67 | 4.91e-3 | -2.31 | |
| 3 | 1.000 | 29.46 | 10.49 | 6.98e-4 | -3.16 | 6.75 | 9.27e-3 | -2.03 | |
NGS expression data
Expression of miR168b transcript in NGS dataSequences
miRNA transcript
UUCUCCUCCUUAUUCUCUUUCACCUUUUUCUGAUGAGAGAGAGAUGCGAUGAUUGUUAAAGUUACCGGCGGUCUCGGAUUCGCUUGGUGCAGGUCGGGAACUGAUUGGCUGACACCGACACGUGUCUUGUCAUGGUUGGUUUGUGAGCUCCCGUCUUGUAUCAACUGAAUCGGAGUCCGAGGUGA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGCUUGGUGCAGGUCGGGAA | miRNA | 80 | 100 | |
TCTCCTCCTTATTCTCTTTCACCTTTT | primer | 5p | 2 | 28 |
GTGTCGGTGTCAGCCAATCA | primer | 3p | 102 | 121 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR168b found in PubMed |