Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 3.93453 | 0.417836 | 4.00704 | 0.308658 | |
| 10day-seedling | 0.96231 | 0.394302 | 2.77047 | 0.285362 | |
| 14day-seedling | 2.85092 | 0.236945 | 4.25605 | 0.604579 | |
| 25day-aerial | 0.7804 | 0.768174 | 2.44284 | 1.07384 | |
| 35day-aerial | 0.65371 | 0.852862 | 2.45153 | 1.86725 | |
| 42day-rosette | 3.21579 | 2.11499 | 5.24896 | 1.97728 | 42day-stem | 1.7993 | 0.0463388 | 3.43708 | 0.000895243 |
| 53day-rosette | 1.07197 | 0.755051 | 2.83375 | 0.696941 | 53day-stem | 2.77254 | 0.783607 | 3.91363 | 0.427444 | 53day-inflores | 2.30948 | 0.270499 | 3.7444 | 0.245484 | 53day-silique | 3.67235 | 0.703761 | 4.81495 | 0.744114 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 34.49 | 10.64 | 6.27e-4 | -3.2 | 10.55 | 6.66e-4 | -3.18 |
| 2 | 1.000 | 36.6 | 8.62 | 2.53e-3 | -2.6 | 8.76 | 2.31e-3 | -2.64 | |
| 3 | 1.000 | 35.56 | 11.29 | 4.00e-4 | -3.4 | 10.52 | 6.82e-4 | -3.17 | |
| 10day-seedling | 1 | 1.000 | 31.41 | 18.55 | 2.61e-6 | -5.58 | 13.05 | 1.18e-4 | -3.93 |
| 2 | 1.000 | 34 | 20.75 | 5.67e-7 | -6.25 | 14.94 | 3.19e-5 | -4.5 | |
| 3 | 1.000 | 32.24 | 20.87 | 5.21e-7 | -6.28 | 14.16 | 5.46e-5 | -4.26 | |
| 14day-seedling | 1 | 0.999 | 27.91 | 14.34 | 4.82e-5 | -4.32 | 7.7 | 4.83e-3 | -2.32 |
| 2 | 1.000 | 31.34 | 13.23 | 1.04e-4 | -3.98 | 10.32 | 7.81e-4 | -3.11 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.704 | 32.9 | 18.86 | 2.11e-6 | -5.68 | 12.61 | 1.60e-4 | -3.8 | |
| 3 | 0.999 | 36.16 | 22.28 | 1.97e-7 | -6.71 | 16.71 | 9.30e-6 | -5.03 | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 29.58 | 16.86 | 8.43e-6 | -5.07 | 10.72 | 5.91e-4 | -3.23 | |
| 3 | 0.998 | 36.28 | 23.09 | 1.12e-7 | -6.95 | 16.9 | 8.19e-6 | -5.09 | |
| 42day-rosette | 1 | 0.995 | 31.08 | 17.54 | 5.25e-6 | -5.28 | 10.43 | 7.23e-4 | -3.14 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.861 | 20.26 | 6.74 | 9.36e-3 | -2.03 | 1.17 | 4.44e-1 | -0.35 | |
| 42day-stem | 1 | 0.998 | 28.84 | 17.14 | 6.96e-6 | -5.17 | 11.83 | 2.74e-1 | -3.56 |
| 2 | 1.000 | 29.93 | 17.39 | 5.84e-1 | -5.23 | 11.84 | 2.73e-1 | -3.56 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-rosette | 1 | 1.000 | 35.52 | 21.47 | 3.45e-7 | -6.46 | 15.48 | 2.19e-5 | -4.66 |
| 2 | 1.000 | 30.21 | 17.92 | 4.04e-6 | -5.39 | 12.2 | 2.12e-4 | -3.67 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-stem | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.999 | 30.66 | 15.88 | 1.65e-5 | -4.78 | 11.23 | 4.16e-4 | -3.38 | |
| 3 | 0.981 | 29.81 | 12.2 | 2.12e-4 | -3.67 | 9.25 | 1.64e-3 | -2.78 | |
| 53day-inflores | 1 | 0.991 | 29.46 | 16.07 | 1.46e-5 | -4.84 | 10.91 | 5.19e-4 | -3.29 |
| 2 | 0.993 | 30.76 | 16.13 | 1.39e-5 | -4.86 | 11.58 | 3.27e-4 | -3.48 | |
| 3 | 1.000 | 28.37 | 14.54 | 4.18e-5 | -4.38 | 9.95 | 1.01e-3 | -3 | |
| 53day-silique | 1 | 1.000 | 28.26 | 8.42 | 2.93e-3 | -2.53 | 4.46 | 4.53e-2 | -1.34 |
| 2 | 1.000 | 32.16 | 12.87 | 1.34e-4 | -3.87 | 9.16 | 1.75e-3 | -2.76 | |
| 3 | 1.000 | 30.85 | 11.88 | 2.65e-4 | -3.58 | 8.15 | 3.52e-3 | -2.45 | |
NGS expression data
Expression of miR166g transcript in NGS dataSequences
miRNA transcript
GUCUCUUUGCCCUGUCACAUGCUUCCUUCUUCUUCUUCUUUUUUACUUACAUAAAGUUGCUUACAUUCUUGUCCUCUUCAUCUUAUUCUUCUUAUAGUGAAGCGAUUUAGGGUUUAGAGGAAUGUUGUUUGGCUCGAGGUCAUGGAGAGUAAUUCGUUAACCCAACUCAAAACUCUAAAUGAUUCUCGGACCAGGCUUCAUUCCCCUCAACCUAUUUUAUCGC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUUCCCC | miRNA | 186 | 206 | |
TCTCTTTGCCCTGTCACATGCT | primer | 5p | 2 | 23 |
TCCTCTAAACCCTAAATCGCTTCAC | primer | 3p | 97 | 121 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR166g found in PubMed |