Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.2505 | 0.688345 | 4.32301 | 0.71972 | |
| 10day-seedling | 2.44311 | 0.182281 | 4.25127 | 0.368545 | |
| 14day-seedling | 2.2648 | 0 | 3.17132 | 0 | |
| 25day-aerial | 0.58434 | 1.01492 | 2.42131 | 1.07484 | |
| 35day-aerial | 1.84468 | 0.407996 | 3.48388 | 0.114193 | |
| 42day-rosette | 2.03614 | 0 | 4.57586 | 0 | 42day-stem | 1.69896 | 0.880556 | 3.12957 | 1.33593 |
| 53day-rosette | 1.72359 | 0.061409 | 3.5661 | 0.160226 | 53day-stem | 2.77331 | 0.782954 | 4.21639 | 0.36125 | 53day-inflores | 3.02899 | 0.840603 | 4.65809 | 0.904872 | 53day-silique | 3.87175 | 0.244965 | 5.01434 | 0.229339 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 35.6 | 11.74 | 2.93e-4 | -3.53 | 11.65 | 3.11e-4 | -3.51 |
| 2 | 1.000 | 35.44 | 7.46 | 5.68e-3 | -2.25 | 7.59 | 5.18e-3 | -2.29 | |
| 3 | 1.000 | 32.47 | 8.2 | 3.39e-3 | -2.47 | 7.43 | 5.79e-3 | -2.24 | |
| 10day-seedling | 1 | 1.000 | 28.32 | 15.46 | 2.21e-5 | -4.66 | 9.97 | 9.97e-4 | -3 |
| 2 | 1.000 | 28.76 | 15.51 | 2.14e-5 | -4.67 | 9.7 | 1.20e-3 | -2.92 | |
| 3 | 1.000 | 25.81 | 14.44 | 4.50e-5 | -4.35 | 7.73 | 4.72e-3 | -2.33 | |
| 14day-seedling | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.998 | 33.73 | 15.62 | 1.98e-5 | -4.7 | 12.72 | 1.48e-4 | -3.83 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 32.97 | 18.93 | 2.00e-6 | -5.7 | 12.69 | 1.52e-4 | -3.82 | |
| 3 | 1.000 | 37.18 | 23.3 | 9.70e-8 | -7.01 | 17.73 | 4.58e-6 | -5.34 | |
| 35day-aerial | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 0.998 | 30.8 | 18.08 | 3.61e-6 | -5.44 | 11.95 | 2.53e-4 | -3.6 | |
| 3 | 0.990 | 29.36 | 16.17 | 1.36e-5 | -4.87 | 9.98 | 9.90e-4 | -3 | |
| 42day-rosette | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.277 | 27.13 | 13.61 | 8.01e-5 | -4.1 | 8.04 | 3.80e-3 | -2.42 | |
| 42day-stem | 1 | 0.992 | 30.62 | 18.93 | 2.68e-6 | -5.7 | 13.62 | 7.96e-1 | -4.99 |
| 2 | 0.999 | 32.14 | 19.6 | 1.26e-1 | -5.91 | 14.58 | 5.89e-1 | -4.23 | |
| 3 | 0.996 | 27.63 | 14.25 | 5.13e-1 | -4.29 | 7.94 | 4.59e-1 | -2.39 | |
| 53day-rosette | 1 | 1.000 | 31.81 | 17.76 | 4.51e-6 | -5.35 | 11.77 | 2.86e-4 | -3.54 |
| 2 | 1.000 | 29.66 | 17.37 | 5.90e-6 | -5.23 | 11.66 | 3.10e-4 | -3.51 | |
| 3 | 1.000 | 30.34 | 17.45 | 5.57e-6 | -5.25 | 10.8 | 5.62e-4 | -3.25 | |
| 53day-stem | 1 | 0.996 | 29.06 | 15.64 | 1.96e-5 | -4.71 | 9.12 | 1.79e-3 | -2.75 |
| 2 | 1.000 | 29.93 | 15.16 | 2.74e-5 | -4.56 | 10.5 | 6.88e-4 | -3.16 | |
| 3 | 0.995 | 28.67 | 11.07 | 4.66e-4 | -3.33 | 8.11 | 3.61e-3 | -2.44 | |
| 53day-inflores | 1 | 0.988 | 29.75 | 16.36 | 1.19e-5 | -4.93 | 11.21 | 4.23e-4 | -3.37 |
| 2 | 1.000 | 25.72 | 11.1 | 4.57e-4 | -3.34 | 6.54 | 1.08e-2 | -1.97 | |
| 3 | 1.000 | 24.01 | 10.18 | 8.61e-4 | -3.06 | 5.59 | 2.07e-2 | -1.68 | |
| 53day-silique | 1 | 1.000 | 30.58 | 10.73 | 5.88e-4 | -3.23 | 6.78 | 9.09e-3 | -2.04 |
| 2 | 1.000 | 28.75 | 9.46 | 1.42e-3 | -2.85 | 5.76 | 1.85e-2 | -1.73 | |
| 3 | 1.000 | 29.95 | 10.98 | 4.95e-4 | -3.31 | 7.25 | 6.58e-3 | -2.18 | |
NGS expression data
Expression of miR166f transcript in NGS dataSequences
miRNA transcript
GCAUGAGGCCGUAACUAAAAGAAAGUUCAGAAGAGAACUUCUCCCUCUUAAUCAUCUCUCAUCUUUUUCUCAUUCUCUCUUCUCUCUCUGUCUUUCUGAGCCAAAAGUUCAGGUGAAUGAUGCCUGGCUCGAGACCAUUCAAUCUCAUGAUCUCAUGAUUAUAACGAUGAUGAUGAUGAUGUCGGACCAGGCUUCAUUCCCCUCAACUU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUUCCCC | miRNA | 182 | 202 | |
CATGAGGCCGTAACTAAAAGAAAGTT | primer | 5p | 2 | 27 |
TCTCGAGCCAGGCATCATTC | primer | 3p | 115 | 134 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR166f found in PubMed |