Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
Read more
Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 4.24326 | 0.562724 | 4.33894 | 0.754982 | |
| 10day-seedling | 3.1486 | 0.217685 | 4.95676 | 0.0274382 | |
| 14day-seedling | 2.67542 | 0.599906 | 4.11181 | 1.04756 | |
| 25day-aerial | 2.66257 | 0.291839 | 4.49671 | 0.263362 | |
| 35day-aerial | 2.77639 | 0.37234 | 4.54865 | 0.30219 | |
| 42day-rosette | 1.94637 | 1.47293 | 4.00851 | 1.21512 | 42day-stem | 2.56891 | 0.287967 | 4.501 | 0.314346 |
| 53day-rosette | 3.68415 | 0.326073 | 5.52666 | 0.277857 | 53day-stem | 3.10985 | 0.722625 | 4.5372 | 0.218668 | 53day-inflores | 2.63111 | 0.248957 | 4.05214 | 0.171898 | 53day-silique | 3.73928 | 0.47703 | 4.88188 | 0.517007 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | 1.000 | 38.46 | 10.48 | 7.00e-4 | -3.15 | 10.61 | 6.38e-4 | -3.19 | |
| 3 | 1.000 | 32.11 | 7.84 | 4.38e-3 | -2.36 | 7.07 | 7.46e-3 | -2.13 | |
| 10day-seedling | 1 | 1.000 | 25.07 | 12.21 | 2.11e-4 | -3.67 | 6.71 | 9.53e-3 | -2.02 |
| 2 | 1.000 | 25.82 | 12.57 | 1.64e-4 | -3.78 | 6.76 | 9.23e-3 | -2.04 | |
| 3 | 1.000 | 24.97 | 13.6 | 8.04e-5 | -4.09 | 6.89 | 8.44e-3 | -2.07 | |
| 14day-seedling | 1 | 1.000 | 25.8 | 12.22 | 2.09e-4 | -3.68 | 5.58 | 2.09e-2 | -1.68 |
| 2 | 0.995 | 32.78 | 14.67 | 3.84e-5 | -4.42 | 11.76 | 2.88e-4 | -3.54 | |
| 3 | 0.999 | 31.37 | 13.58 | 8.18e-5 | -4.09 | 11.44 | 3.60e-4 | -3.44 | |
| 25day-aerial | 1 | 0.999 | 26.2 | 13.34 | 9.66e-5 | -4.01 | 7.58 | 5.23e-3 | -2.28 |
| 2 | 1.000 | 29.57 | 15.53 | 2.12e-5 | -4.67 | 9.28 | 1.61e-3 | -2.79 | |
| 3 | 1.000 | 27.53 | 13.65 | 7.78e-5 | -4.11 | 8.09 | 3.68e-3 | -2.43 | |
| 35day-aerial | 1 | 0.999 | 27.18 | 12.72 | 1.48e-4 | -3.83 | 7.18 | 6.88e-3 | -2.16 |
| 2 | 1.000 | 26.92 | 14.19 | 5.34e-5 | -4.27 | 8.06 | 3.75e-3 | -2.43 | |
| 3 | 0.997 | 28.37 | 15.18 | 2.70e-5 | -4.57 | 8.99 | 1.97e-3 | -2.71 | |
| 42day-rosette | 1 | 0.999 | 28.29 | 14.75 | 3.63e-5 | -4.44 | 7.64 | 5.00e-3 | -2.3 |
| 2 | 0.997 | 27.61 | 13.22 | 1.04e-4 | -3.98 | 7.53 | 5.40e-3 | -2.27 | |
| 3 | 0.998 | 33.69 | 20.17 | 8.50e-7 | -6.07 | 14.6 | 4.03e-5 | -4.39 | |
| 42day-stem | 1 | 1.000 | 25.4 | 13.77 | 7.48e-1 | -4.13 | 8.4 | 2.97e-1 | -2.53 |
| 2 | 0.998 | 27.39 | 14.84 | 3.42e-1 | -4.47 | 9.29 | 1.59e-1 | -2.8 | |
| 3 | 0.999 | 26.9 | 13.52 | 8.51e-1 | -4.7 | 7.21 | 6.74e-1 | -2.17 | |
| 53day-rosette | 1 | 1.000 | 23.88 | 9.83 | 1.10e-3 | -2.96 | 3.84 | 6.99e-2 | -1.16 |
| 2 | 1.000 | 23.55 | 11.27 | 4.06e-4 | -3.39 | 5.55 | 2.13e-2 | -1.67 | |
| 3 | 1.000 | 24.84 | 11.95 | 2.53e-4 | -3.6 | 5.29 | 2.55e-2 | -1.59 | |
| 53day-stem | 1 | 1.000 | 28.65 | 15.23 | 2.61e-5 | -4.58 | 8.71 | 2.39e-3 | -2.62 |
| 2 | 1.000 | 27.88 | 13.11 | 1.13e-4 | -3.95 | 8.46 | 2.85e-3 | -2.55 | |
| 3 | 0.996 | 27.92 | 10.31 | 7.87e-4 | -3.1 | 7.36 | 6.10e-3 | -2.21 | |
| 53day-inflores | 1 | 0.997 | 28.86 | 15.47 | 2.21e-5 | -4.66 | 10.31 | 7.86e-4 | -3.1 |
| 2 | 0.993 | 28.63 | 14 | 6.09e-5 | -4.22 | 9.45 | 1.43e-3 | -2.84 | |
| 3 | 0.986 | 27.9 | 14.07 | 5.82e-5 | -4.24 | 9.48 | 1.40e-3 | -2.85 | |
| 53day-silique | 1 | 1.000 | 28.85 | 9.01 | 1.94e-3 | -2.71 | 5.06 | 3.00e-2 | -1.52 |
| 2 | 1.000 | 30.91 | 11.62 | 3.18e-4 | -3.5 | 7.91 | 4.14e-3 | -2.38 | |
| 3 | 1.000 | 30.84 | 11.87 | 2.67e-4 | -3.57 | 8.14 | 3.55e-3 | -2.45 | |
NGS expression data
Expression of miR166e transcript in NGS dataSequences
miRNA transcript
UGCUUUCUUGUCUCCUCCUCUUUCAGCUUUUAUUUCUUUAUAAAUUAAGCUCAUCUCUCUCUCUCUCUCUGUUAAAAGCUAGGGUUUUCUUAUAUGGAGUCCAUGUUCUUCAGUGAAGCUUUUCCUUUUGAGGGGAAUGUUGUCUGGCACGAGGCCCUUAACUUAGAUCUAUAUUUGAUUAUAUAUAUAUGUCUCUUCUUUAUUCAUUAGUCUAUACAUGAAUGAUCAUUUUACGGUUAAUGACGUCGGACCAGGCUUCAUUCCCCUCAA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUUCCCC | miRNA | 246 | 266 | |
GCTTTCTTGTCTCCTCCTCTTTCAG | primer | 5p | 2 | 26 |
GCCAGACAACATTCCCCTCAA | primer | 3p | 128 | 148 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR166e found in PubMed |