Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.06391 | 0.791993 | 5.19281 | 0.937263 | |
| 10day-seedling | 3.1613 | 0.225113 | 4.96945 | 0.0685368 | |
| 14day-seedling | 1.56804 | 1.35714 | 3.03096 | 1.4211 | |
| 25day-aerial | 2.57618 | 0.24316 | 4.35963 | 0.328448 | |
| 35day-aerial | 2.67106 | 0.137836 | 4.59848 | 0.166491 | |
| 42day-rosette | 2.8564 | 0.151763 | 4.69646 | 0.179577 | 42day-stem | 2.52556 | 0.454146 | 4.5426 | 0.22526 |
| 53day-rosette | 3.60794 | 0.810316 | 5.45045 | 0.764248 | 53day-stem | 3.15839 | 0.443964 | 4.83994 | 0.0467663 | 53day-inflores | 3.34156 | 0.09357 | 4.77668 | 0.114432 | 53day-silique | 3.71929 | 0.135654 | 4.87147 | 0.188028 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 32.15 | 8.29 | 3.19e-3 | -2.5 | 8.2 | 3.39e-3 | -2.47 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 28.84 | 4.57 | 4.21e-2 | -1.38 | 3.8 | 7.17e-2 | -1.14 | |
| 10day-seedling | 1 | 1.000 | 24.86 | 12 | 2.45e-4 | -3.61 | 6.5 | 1.10e-2 | -1.96 |
| 2 | 1.000 | 26.02 | 12.77 | 1.43e-4 | -3.84 | 6.95 | 8.06e-3 | -2.09 | |
| 3 | 1.000 | 24.86 | 13.49 | 8.68e-5 | -4.06 | 6.78 | 9.10e-3 | -2.04 | |
| 14day-seedling | 1 | 1.000 | 28.27 | 14.7 | 3.75e-5 | -4.43 | 8.06 | 3.75e-3 | -2.43 |
| 2 | 0.996 | 38.37 | 20.26 | 7.99e-7 | -6.1 | 17.35 | 5.98e-6 | -5.22 | |
| 3 | 0.999 | 34.05 | 16.26 | 1.27e-5 | -4.89 | 14.12 | 5.61e-5 | -4.25 | |
| 25day-aerial | 1 | 1.000 | 28.28 | 15.42 | 2.28e-5 | -4.64 | 9.66 | 1.23e-3 | -2.91 |
| 2 | 0.999 | 28.88 | 14.84 | 3.42e-5 | -4.47 | 8.59 | 2.59e-3 | -2.59 | |
| 3 | 0.999 | 27.22 | 13.34 | 9.64e-5 | -4.02 | 7.78 | 4.56e-3 | -2.34 | |
| 35day-aerial | 1 | 1.000 | 28.4 | 13.94 | 6.35e-5 | -4.2 | 8.41 | 2.95e-3 | -2.53 |
| 2 | 0.998 | 26.17 | 13.44 | 8.97e-5 | -4.05 | 7.31 | 6.29e-3 | -2.2 | |
| 3 | 0.999 | 27.53 | 14.34 | 4.82e-5 | -4.32 | 8.15 | 3.51e-3 | -2.45 | |
| 42day-rosette | 1 | 1.000 | 27.69 | 14.15 | 5.50e-5 | -4.26 | 7.04 | 7.57e-3 | -2.12 |
| 2 | 0.999 | 28.33 | 13.95 | 6.32e-5 | -4.2 | 8.26 | 3.27e-3 | -2.49 | |
| 3 | 1.000 | 26.72 | 13.19 | 1.07e-4 | -3.97 | 7.63 | 5.06e-3 | -2.3 | |
| 42day-stem | 1 | 1.000 | 25.83 | 14.14 | 5.53e-1 | -4.26 | 8.83 | 2.19e-1 | -2.66 |
| 2 | 0.987 | 26.39 | 13.86 | 6.77e-1 | -4.17 | 8.32 | 3.17e-1 | -2.5 | |
| 3 | 0.996 | 27 | 13.62 | 7.95e-1 | -5 | 7.31 | 6.29e-1 | -2.21 | |
| 53day-rosette | 1 | 1.000 | 22.25 | 8.21 | 3.39e-3 | -2.47 | 2.22 | 2.15e-1 | -0.67 |
| 2 | 1.000 | 24.63 | 12.34 | 1.92e-4 | -3.72 | 6.63 | 1.01e-2 | -1.99 | |
| 3 | 1.000 | 26.15 | 13.26 | 1.02e-4 | -3.99 | 6.6 | 1.03e-2 | -1.99 | |
| 53day-stem | 1 | 0.999 | 27.23 | 13.8 | 6.99e-5 | -4.16 | 7.29 | 6.41e-3 | -2.19 |
| 2 | 1.000 | 26.49 | 11.72 | 2.97e-4 | -3.53 | 7.07 | 7.46e-3 | -2.13 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 53day-inflores | 1 | 0.995 | 25.67 | 12.28 | 2.01e-4 | -3.7 | 7.13 | 7.15e-3 | -2.15 |
| 2 | 0.997 | 27.01 | 12.38 | 1.88e-4 | -3.73 | 7.82 | 4.42e-3 | -2.35 | |
| 3 | 9.999 | 25.63 | 11.8 | 2.81e-4 | -3.55 | 7.21 | 6.77e-3 | -2.17 | |
| 53day-silique | 1 | 1.000 | 30.43 | 10.58 | 6.53e-4 | -3.18 | 6.63 | 1.01e-2 | -2 |
| 2 | 1.000 | 30.51 | 11.22 | 4.20e-4 | -3.38 | 7.51 | 5.48e-3 | -2.26 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
NGS expression data
Expression of miR166c transcript in NGS dataSequences
miRNA transcript
UGCGAUUUAGUGUUGAGAGGAUUGUUGUCUGGCUCGAGGUCAUGAAGAAGAGAAUCACUCGAAUUAAUUUGGAAGAACAAAUUAAGAAAACCCUAGAUGAUUCUCGGACCAGGCUUCAUUCCCCCUAACCUACUUAUCGCUUUUCGCCCACUUUCUUUCUUUAGUCUUUUUUGUCUUAGUUUCAUAUUUGUUCUAUCUUGCGACCGA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUUCCCC | miRNA | 104 | 124 | |
TGCGATTTAGTGTTGAGAGGATTG | primer | 5p | 1 | 24 |
CGGTCGCAAGATAGAACAAATATGA | primer | 3p | 182 | 206 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR166c found in PubMed |