Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.00906 | 0.46194 | 5.08157 | 0.595499 | |
| 10day-seedling | 4.09712 | 0.597858 | 5.90528 | 0.447608 | |
| 14day-seedling | 3.32372 | 0.559409 | 4.49621 | 0.86707 | |
| 25day-aerial | 3.6493 | 0.638778 | 5.41183 | 0.593149 | |
| 35day-aerial | 3.61022 | 0.367889 | 5.37973 | 0.229482 | |
| 42day-rosette | 3.67283 | 0.318714 | 5.53315 | 0.506924 | 42day-stem | 3.6284 | 0.528591 | 5.62505 | 0.262373 |
| 53day-rosette | 4.0756 | 0.689775 | 5.91811 | 0.62696 | 53day-stem | 4.4366 | 0.38031 | 5.84033 | 0.177014 | 53day-inflores | 4.55435 | 0.130654 | 5.78834 | 0.261725 | 53day-silique | 5.03728 | 0.0517401 | 6.179873 | 0.048392 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 30.29 | 6.43 | 1.16e-2 | -1.94 | 6.35 | 1.23e-2 | -1.91 |
| 2 | 1.000 | 36.21 | 8.23 | 3.33e-3 | -2.48 | 8.36 | 3.03e-3 | -2.52 | |
| 3 | 1.000 | 29.45 | 5.18 | 2.76e-2 | -1.56 | 4.41 | 4.71e-2 | -1.33 | |
| 10day-seedling | 1 | 1.000 | 20.27 | 7.41 | 5.86e-3 | -2.23 | 1.92 | 2.64e-1 | -0.58 |
| 2 | 1.000 | 23.54 | 10.29 | 7.97e-4 | -3.1 | 4.48 | 4.48e-2 | -1.35 | |
| 3 | 1.000 | 22.59 | 11.22 | 4.18e-4 | -3.38 | 4.51 | 4.39e-2 | -1.36 | |
| 14day-seedling | 1 | 0.998 | 25.67 | 12.1 | 2.27e-4 | -3.64 | 5.46 | 2.28e-2 | -1.64 |
| 2 | 0.996 | 32.23 | 14.12 | 5.60e-5 | -4.25 | 11.22 | 4.20e-4 | -3.38 | |
| 3 | 0.979 | 28.2 | 10.41 | 7.34e-4 | -3.13 | 8.27 | 3.24e-3 | -2.49 | |
| 25day-aerial | 1 | 0.998 | 25.76 | 12.9 | 1.31e-4 | -3.88 | 7.14 | 7.08e-3 | -2.15 |
| 2 | 0.999 | 25.76 | 11.71 | 2.98e-4 | -3.53 | 5.47 | 2.26e-2 | -1.65 | |
| 3 | 0.970 | 22.66 | 8.78 | 2.28e-3 | -2.64 | 3.22 | 1.08e-1 | -0.97 | |
| 35day-aerial | 1 | 0.995 | 24.34 | 9.88 | 1.06e-3 | -2.97 | 4.34 | 4.93e-2 | -1.31 |
| 2 | 0.988 | 24.93 | 12.2 | 2.12e-4 | -3.67 | 6.07 | 1.49e-2 | -1.83 | |
| 3 | 0.998 | 24.89 | 11.7 | 3.01e-4 | -3.52 | 5.51 | 2.19e-2 | -1.66 | |
| 42day-rosette | 1 | 0.999 | 23.86 | 10.32 | 7.84e-4 | -3.11 | 3.21 | 1.08e-1 | -0.97 |
| 2 | 0.997 | 26.66 | 12.27 | 2.02e-4 | -3.69 | 6.58 | 1.04e-2 | -1.98 | |
| 3 | 0.999 | 23.91 | 10.39 | 7.46e-4 | -3.13 | 4.82 | 3.54e-2 | -1.45 | |
| 42day-stem | 1 | 0.999 | 21.18 | 9.48 | 1.40e-1 | -2.86 | 4.17 | 5.54e-1 | -1.26 |
| 2 | 0.586 | 23.66 | 11.12 | 4.57e-1 | -3.35 | 5.57 | 2.11e-1 | -1.68 | |
| 3 | 0.994 | 23.65 | 1.27 | 8.15e-1 | -3.91 | 3.96 | 6.42e-1 | -1.19 | |
| 53day-rosette | 1 | 1.000 | 21.24 | 7.19 | 6.84e-3 | -2.16 | 1.2 | 4.35e-1 | -0.36 |
| 2 | 1.000 | 22.57 | 10.29 | 8.00e-4 | -3.1 | 4.57 | 4.21e-2 | -1.38 | |
| 3 | 1.000 | 24.56 | 11.67 | 3.08e-4 | -3.51 | 5.01 | 3.11e-2 | -1.51 | |
| 53day-stem | 1 | 0.996 | 23.23 | 9.81 | 1.11e-3 | -2.95 | 3.29 | 1.02e-1 | -0.99 |
| 2 | 1.000 | 23.22 | 8.45 | 2.86e-3 | -2.54 | 3.79 | 7.21e-2 | -1.14 | |
| 3 | 0.982 | 24.89 | 7.29 | 6.40e-3 | -2.19 | 4.33 | 4.96e-2 | -1.3 | |
| 53day-inflores | 1 | 0.999 | 21.97 | 8.57 | 2.62e-3 | -2.58 | 3.42 | 9.35e-2 | -1.03 |
| 2 | 0.999 | 22.65 | 8.02 | 3.86e-3 | -2.41 | 3.46 | 9.07e-2 | -1.04 | |
| 3 | 0.958 | 21.47 | 7.64 | 5.01e-3 | -2.3 | 3.05 | 1.21e-1 | -0.92 | |
| 53day-silique | 1 | 1.000 | 26.44 | 6.6 | 1.03e-2 | -1.99 | 2.64 | 1.60e-1 | -0.8 |
| 2 | 1.000 | 25.62 | 6.32 | 1.25e-2 | -1.9 | 2.62 | 1.63e-1 | -0.79 | |
| 3 | 1.000 | 25.61 | 6.64 | 1.00e-2 | -2 | 2.91 | 1.33e-1 | -0.88 | |
NGS expression data
Expression of miR166b transcript in NGS dataSequences
miRNA transcript
UUUUCUUUUGAGGGGACUGUUGUCUGGCUCGAGGACUCUUAUUCUAAUACAAUCUCAUUUGAAUACAUUCAGAUCUGAUGAUUGAUUAGGGUUUUAGUGUCGUCGGACCAGGCUUCAUUCCCCCCAAUUAUCACUCCCUCACAAUCCGAAUU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUUCCCC | miRNA | 103 | 123 | |
TTTCTTTTGAGGGGACTGTTG | primer | 5p | 2 | 22 |
ATTCGGATTGTGAGGGAGTG | primer | 3p | 132 | 151 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR166b found in PubMed |