Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.57669 | 0.411662 | 5.64921 | 0.553221 | |
| 10day-seedling | 4.48018 | 0.517315 | 6.288335 | 0.335897 | |
| 14day-seedling | 4.24509 | 1.06935 | 5.42083 | 0.358004 | |
| 25day-aerial | 4.25086 | 0.189096 | 5.74393 | 0.333961 | |
| 35day-aerial | 4.34121 | 0.0631534 | 6.141065 | 0.0831098 | |
| 42day-rosette | 4.61689 | 0.13279 | 6.459605 | 0.146003 | 42day-stem | 4.55588 | 0.103895 | 6.279659 | 0.147949 |
| 53day-rosette | 4.68007 | 0.273772 | 6.52257 | 0.251318 | 53day-stem | 4.85764 | 0.528853 | 6.419052 | 0.115437 | 53day-inflores | 4.55734 | 0.340155 | 6.235917 | 0.0612587 | 53day-silique | 5.18386 | 0.247178 | 6.326453 | 0.2242 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 29.12 | 5.26 | 2.60e-2 | -1.58 | 5.18 | 2.76e-2 | -1.56 |
| 2 | 1.000 | 33.72 | 5.75 | 1.86e-2 | -1.73 | 5.88 | 1.70e-2 | -1.77 | |
| 3 | 1.000 | 27.45 | 3.17 | 1.11e-1 | -0.96 | 2.4 | 1.89e-1 | -0.72 | |
| 10day-seedling | 1 | 1.000 | 19.54 | 6.68 | 9.78e-3 | -2.01 | 1.18 | 4.41e-1 | -0.36 |
| 2 | 1.000 | 21.57 | 8.32 | 3.12e-3 | -2.51 | 2.51 | 1.76e-1 | -0.76 | |
| 3 | 1.000 | 21.48 | 10.11 | 9.03e-4 | -3.04 | 3.4 | 9.47e-2 | -1.02 | |
| 14day-seedling | 1 | 0.990 | 23.85 | 10.28 | 8.07e-4 | -3.09 | 3.63 | 8.07e-2 | -1.09 |
| 2 | 0.997 | 26.31 | 8.2 | 3.40e-3 | -2.47 | 5.3 | 2.54e-2 | -1.59 | |
| 3 | 0.999 | 23.96 | 6.17 | 1.39e-2 | -1.86 | 4.03 | 6.11e-2 | -1.21 | |
| 25day-aerial | 1 | 1.000 | 22.11 | 9.25 | 1.65e-3 | -2.78 | 3.49 | 8.91e-2 | -1.05 |
| 2 | 1.000 | 23.74 | 9.7 | 1.21e-3 | -2.92 | 3.45 | 9.14e-2 | -1.04 | |
| 3 | 1.000 | 22.33 | 8.45 | 2.85e-3 | -2.55 | 2.89 | 1.35e-1 | -0.87 | |
| 35day-aerial | 1 | 0.997 | 23.1 | 8.65 | 2.50e-3 | -2.6 | 3.11 | 1.16e-1 | -0.94 |
| 2 | 0.997 | 21.41 | 8.69 | 2.42e-3 | -2.62 | 2.56 | 1.70e-1 | -0.77 | |
| 3 | 0.992 | 22.27 | 9.07 | 1.86e-3 | -2.73 | 2.89 | 1.35e-1 | -0.87 | |
| 42day-rosette | 1 | 1.000 | 21.9 | 8.37 | 3.03e-3 | -2.52 | 1.26 | 4.17e-1 | -0.38 |
| 2 | 0.996 | 22.28 | 7.9 | 4.19e-3 | -2.38 | 2.21 | 2.16e-1 | -0.66 | |
| 3 | 1.000 | 21.01 | 7.48 | 5.59e-3 | -2.25 | 1.92 | 2.65e-1 | -0.58 | |
| 42day-stem | 1 | 0.999 | 19.44 | 7.74 | 4.66e-1 | -2.33 | 2.43 | 1.85e-1 | -0.73 |
| 2 | 0.999 | 20.95 | 8.46 | 2.95e-1 | -2.54 | 2.86 | 1.38e-1 | -0.86 | |
| 3 | 0.995 | 21.57 | 8.19 | 3.43e-1 | -2.47 | 1.88 | 2.71e-1 | -0.57 | |
| 53day-rosette | 1 | 1.000 | 20.72 | 6.68 | 9.79e-3 | -2.01 | 0.69 | 6.21e-1 | -0.21 |
| 2 | 1.000 | 20.34 | 8.05 | 3.77e-3 | -2.42 | 2.34 | 1.98e-1 | -0.7 | |
| 3 | 1.000 | 21.28 | 8.39 | 2.97e-3 | -2.53 | 1.74 | 3.00e-1 | -0.52 | |
| 53day-stem | 1 | 1.000 | 21.41 | 7.98 | 3.95e-3 | -2.4 | 1.46 | 3.62e-1 | -0.44 |
| 2 | 0.999 | 21.57 | 6.8 | 8.99e-3 | -2.05 | 2.14 | 2.26e-1 | -0.65 | |
| 3 | 0.997 | 22.69 | 5.08 | 2.95e-2 | -1.53 | 2.13 | 2.29e-1 | -0.64 | |
| 53day-inflores | 1 | 1.000 | 21.27 | 7.88 | 4.24e-3 | -2.37 | 2.73 | 1.51e-1 | -0.82 |
| 2 | 0.999 | 21.75 | 7.12 | 7.20e-3 | -2.14 | 2.56 | 1.69e-1 | -0.77 | |
| 3 | 1.000 | 20.74 | 6.91 | 8.29e-3 | -2.08 | 2.32 | 2.00e-1 | -0.7 | |
| 53day-silique | 1 | 1.000 | 26.39 | 6.54 | 1.07e-2 | -1.97 | 2.59 | 1.66e-1 | -0.78 |
| 2 | 1.000 | 24.38 | 5.09 | 2.94e-2 | -1.53 | 1.38 | 3.84e-1 | -0.42 | |
| 3 | 1.000 | 25.44 | 6.47 | 1.13e-2 | -1.95 | 2.74 | 1.50e-1 | -0.82 | |
NGS expression data
Expression of miR166a transcript in NGS dataSequences
miRNA transcript
AGGGGCUUUCUCUUUUGAGGGGACUGUUGUCUGGCUCGAGGACUCUGGCUCGCUCUAUUCAUGUUGGAUCUCUUUCGAUCUAACAAUCGAAUUGAACCUUCAGAUUUCAGAUUUGAUUAGGGUUUUAGCGUCUUCGGACCAGGCUUCAUUCCCCCCAAUUGUUGCUCCCUGUUUACUCCAU
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGGACCAGGCUUCAUUCCCC | miRNA | 134 | 154 | |
CTGGCTCGAGGACTCTGG | primer | 5p | 31 | 48 |
TGGAGTAAACAGGGAGCAACA | primer | 3p | 160 | 180 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR166a found in PubMed |