Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
Read more
Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.50412 | 0.595201 | 5.57664 | 0.570525 | |
| 10day-seedling | 2.94201 | 0.487111 | 4.75017 | 0.336645 | |
| 14day-seedling | 2.44264 | 0.463158 | 3.87988 | 0.332825 | |
| 25day-aerial | 2.41314 | 0.583578 | 4.20118 | 0.491738 | |
| 35day-aerial | 2.95142 | 0.68023 | 5.01447 | 0.224445 | |
| 42day-rosette | 3.17159 | 0.704002 | 5.01403 | 0.770957 | 42day-stem | 3.0948 | 0.601182 | 4.6791 | 0.45653 |
| 53day-rosette | 3.21124 | 0.731035 | 5.05374 | 0.58604 | 53day-stem | 4.68933 | 1.52203 | 6.103678 | 1.24536 | 53day-inflores | 3.86897 | 0.238699 | 5.30387 | 0.208502 | 53day-silique | 5.75997 | 0.102908 | 6.9025647 | 0.107024 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | 1.000 | 30.73 | 6.88 | 8.50e-3 | -2.07 | 6.79 | 9.03e-3 | -2.04 |
| 2 | 1.000 | 30.91 | 2.93 | 1.31e-1 | -0.88 | 3.06 | 1.20e-1 | -0.92 | |
| 3 | 1.000 | 29.37 | 5.1 | 2.92e-2 | -1.54 | 4.33 | 4.97e-2 | -1.3 | |
| 10day-seedling | 1 | 1.000 | 24.54 | 11.68 | 3.05e-4 | -3.52 | 6.18 | 1.38e-2 | -1.86 |
| 2 | 1.000 | 27.21 | 13.96 | 6.28e-5 | -4.2 | 8.15 | 3.53e-3 | -2.45 | |
| 3 | 1.000 | 26.17 | 14.81 | 3.49e-5 | -4.46 | 8.09 | 3.66e-3 | -2.44 | |
| 14day-seedling | 1 | 0.997 | 29.8 | 16.23 | 1.30e-5 | -4.88 | 9.58 | 1.30e-3 | -2.88 |
| 2 | 0.980 | 32.16 | 14.05 | 5.89e-5 | -4.23 | 11.15 | 4.41e-4 | -3.36 | |
| 3 | - | - | - | - | N/D | - | - | N/D | |
| 25day-aerial | 1 | 1.000 | 29.16 | 16.31 | 1.23e-5 | -4.91 | 10.55 | 6.69e-4 | -3.17 |
| 2 | 0.998 | 30.18 | 16.14 | 1.39e-5 | -4.86 | 9.9 | 1.05e-3 | -2.98 | |
| 3 | 1.000 | 26.89 | 13.01 | 1.21e-4 | -3.92 | 7.45 | 5.72e-3 | -2.24 | |
| 35day-aerial | 1 | 0.999 | 26.44 | 11.99 | 2.47e-4 | -3.61 | 6.45 | 1.15e-2 | -1.94 |
| 2 | 0.998 | 24.79 | 12.07 | 2.33e-4 | -3.63 | 5.93 | 1.63e-2 | -1.79 | |
| 3 | 0.985 | 26.76 | 13.56 | 8.25e-5 | -4.08 | 7.38 | 6.02e-3 | -2.22 | |
| 42day-rosette | 1 | 1.000 | 25.82 | 12.28 | 2.00e-4 | -3.7 | 5.18 | 2.76e-2 | -1.56 |
| 2 | 0.996 | 29.62 | 15.24 | 2.58e-5 | -4.59 | 9.55 | 1.33e-3 | -2.88 | |
| 3 | 1.000 | 24.15 | 10.63 | 6.33e-4 | -3.2 | 5.06 | 3.00e-2 | -1.52 | |
| 42day-stem | 1 | 0.999 | 23.78 | 12.89 | 2.30e-1 | -3.64 | 6.78 | 9.18e-1 | -2.46 |
| 2 | 0.998 | 24.11 | 11.57 | 3.29e-1 | -3.48 | 6.2 | 1.54e-1 | -1.81 | |
| 3 | 0.999 | 28.64 | 15.26 | 2.55e-1 | -4.59 | 8.95 | 2.20e-1 | -2.69 | |
| 53day-rosette | 1 | 1.000 | 26.22 | 12.17 | 2.16e-4 | -3.66 | 6.18 | 1.37e-2 | -1.86 |
| 2 | 1.000 | 22.68 | 10.39 | 7.45e-4 | -3.13 | 4.67 | 3.92e-2 | -1.41 | |
| 3 | 1.000 | 28.09 | 15.19 | 2.67e-5 | -4.57 | 8.54 | 2.69e-3 | -2.57 | |
| 53day-stem | 1 | 0.999 | 22.34 | 8.91 | 2.07e-3 | -2.68 | 2.4 | 1.90e-1 | -0.72 |
| 2 | 1.000 | 26.77 | 12 | 2.45e-4 | -3.61 | 7.34 | 6.15e-3 | -2.21 | |
| 3 | 0.996 | 19.72 | 2.12 | 2.31e-1 | -0.64 | -0.84 | 1.79e+0 | 0.25 | |
| 53day-inflores | 1 | 0.992 | 24.28 | 10.89 | 5.27e-4 | -3.28 | 5.74 | 1.88e-2 | -1.73 |
| 2 | 1.000 | 25.46 | 10.83 | 5.51e-4 | -3.26 | 6.27 | 1.30e-2 | -1.89 | |
| 3 | 1.000 | 23.32 | 9.49 | 1.39e-3 | -2.86 | 4.9 | 3.36e-2 | -1.47 | |
| 53day-silique | 1 | 1.000 | 23.96 | 4.12 | 5.77e-2 | -1.24 | 0.17 | 8.92e-1 | -0.05 |
| 2 | 1.000 | 23.07 | 3.78 | 7.28e-2 | -1.14 | 0.07 | 9.50e-1 | -0.02 | |
| 3 | 1.000 | 23.43 | 4.46 | 4.54e-2 | -1.34 | 0.73 | 6.03e-1 | -0.22 | |
NGS expression data
Expression of miR162b transcript in NGS dataSequences
miRNA transcript
GGAAAGAGUGAAGUCGCUGGAGGCAGCGGUUCAUCGAUCAAUUCCUGUGAAUAUUUAUUUUUGUUUACAAAAGCAAGAAUCGAUCGAUAAACCUCUGCAUCCAGCGCUGCUUGCUCUUCAUGACUUUUGUUUAAUUGAUAUCUAUUUGAGUAUUGCUGAGAAGCAGGACAAUGGAUUCGAUA
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UCGAUAAACCUCUGCAUCCAG | miRNA | 84 | 104 | |
GAAAGAGTGAAGTCGCTGGAG | primer | 5p | 2 | 22 |
ATCGAATCCATTGTCCTGCT | primer | 3p | 162 | 181 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR162b found in PubMed |