Expression
Switch to: Actin
In most cases the level of expression of pre-miRNA is lower than reference genes. To avoid showing the expression level as a negative value, the graph below is rescaled and the zero value on the y-axis corresponds to minimal expression level in the whole experiment (-7).
The expression values are rescaled. 
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Expression Data
| Development stage | actin | PP2A | |||
|---|---|---|---|---|---|
| Log | SD | Log | SD | ||
| seed | 5.24251 | 0 | 5.47414 | 0 | |
| 10day-seedling | 4.37174 | 0.516167 | 6.1799 | 0.481167 | |
| 14day-seedling | 3.32264 | 0.698617 | 4.64464 | 1.65758 | |
| 25day-aerial | 3.69296 | 0.320343 | 5.45853 | 0.260352 | |
| 35day-aerial | 4.20177 | 0.326029 | 5.9883 | 0.260877 | |
| 42day-rosette | 3.80201 | 0.560738 | 5.75282 | 0.761979 | 42day-stem | 4.34603 | 0.15836 | 6.068514 | 0.253621 |
| 53day-rosette | 4.59066 | 0.187266 | 6.433168 | 0.136546 | 53day-stem | 4.75744 | 0.344288 | 6.173394 | 0.251896 | 53day-inflores | 4.49373 | 0.266164 | 5.91746 | 0.242631 | 53day-silique | 3.6178 | 0.371702 | 4.7604 | 0.400675 |
Raw Data
| Development stage | rep | R2 | Ct | actin | PP2A | ||||
|---|---|---|---|---|---|---|---|---|---|
| ΔCt | 2^(-ΔCt) | log10 | ΔCt | 2^(-ΔCt) | log10 | ||||
| seed | 1 | - | - | - | - | N/D | - | - | N/D |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 1.000 | 30.11 | 5.84 | 1.75e-2 | -1.76 | 5.07 | 2.98e-2 | -1.53 | |
| 10day-seedling | 1 | 1.000 | 19.77 | 6.91 | 8.29e-3 | -2.08 | 1.42 | 3.74e-1 | -0.43 |
| 2 | 1.000 | 23.57 | 10.32 | 7.82e-4 | -3.11 | 4.51 | 4.40e-2 | -1.36 | |
| 3 | 1.000 | 20.33 | 8.96 | 2.01e-3 | -2.7 | 2.25 | 2.11e-1 | -0.68 | |
| 14day-seedling | 1 | 1.000 | 24.15 | 10.57 | 6.56e-4 | -3.18 | 3.93 | 6.56e-2 | -1.18 |
| 2 | - | - | - | - | N/D | - | - | N/D | |
| 3 | 0.999 | 31.65 | 13.86 | 6.74e-5 | -4.17 | 11.72 | 2.97e-4 | -3.53 | |
| 25day-aerial | 1 | 1.000 | 24.53 | 11.67 | 3.07e-4 | -3.51 | 5.91 | 1.66e-2 | -1.78 |
| 2 | 0.995 | 25.54 | 11.5 | 3.46e-4 | -3.46 | 5.25 | 2.62e-2 | -1.58 | |
| 3 | 0.999 | 23.64 | 9.76 | 1.15e-3 | -2.94 | 4.2 | 5.45e-2 | -1.26 | |
| 35day-aerial | 1 | 1.000 | 22.88 | 8.43 | 2.90e-3 | -2.54 | 2.89 | 1.35e-1 | -0.87 |
| 2 | 0.997 | 21.67 | 8.95 | 2.03e-3 | -2.69 | 2.81 | 1.42e-1 | -0.85 | |
| 3 | 0.993 | 23.7 | 10.51 | 6.86e-4 | -3.16 | 4.32 | 5.00e-2 | -1.3 | |
| 42day-rosette | 1 | 0.997 | 22.09 | 8.55 | 2.67e-3 | -2.57 | 1.44 | 3.68e-1 | -0.43 |
| 2 | 1.000 | 26.54 | 12.15 | 2.20e-4 | -3.66 | 6.46 | 1.13e-2 | -1.95 | |
| 3 | 1.000 | 23.61 | 10.09 | 9.20e-4 | -3.04 | 4.52 | 4.36e-2 | -1.36 | |
| 42day-stem | 1 | 0.998 | 20.21 | 8.52 | 2.73e-1 | -2.56 | 3.27 | 1.83e-1 | -0.97 |
| 2 | 0.997 | 21.97 | 9.42 | 1.46e-1 | -2.84 | 3.87 | 6.82e-1 | -1.17 | |
| 3 | 0.999 | 21.89 | 8.58 | 2.75e-1 | -2.56 | 2.21 | 2.17e-1 | -0.66 | |
| 53day-rosette | 1 | 1.000 | 21.43 | 7.38 | 6.00e-3 | -2.22 | 1.39 | 3.81e-1 | -0.42 |
| 2 | 1.000 | 20.29 | 8 | 3.90e-3 | -2.41 | 2.29 | 2.05e-1 | -0.69 | |
| 3 | 1.000 | 21.52 | 8.63 | 2.53e-3 | -2.6 | 1.97 | 2.55e-1 | -0.59 | |
| 53day-stem | 1 | 1.000 | 22.07 | 8.65 | 2.49e-3 | -2.6 | 2.13 | 2.29e-1 | -0.64 |
| 2 | 1.000 | 21.81 | 7.04 | 7.62e-3 | -2.12 | 2.38 | 1.92e-1 | -0.72 | |
| 3 | 0.999 | 23.92 | 6.31 | 1.26e-2 | -1.9 | 3.36 | 9.74e-2 | -1.01 | |
| 53day-inflores | 1 | 1.000 | 22.21 | 8.82 | 2.21e-3 | -2.66 | 3.67 | 7.88e-2 | -1.1 |
| 2 | 0.999 | 23.48 | 8.85 | 2.16e-3 | -2.66 | 4.3 | 5.09e-2 | -1.29 | |
| 3 | 0.998 | 21.13 | 7.3 | 6.32e-3 | -2.2 | 2.71 | 1.52e-1 | -0.82 | |
| 53day-silique | 1 | 1.000 | 30.21 | 10.37 | 7.57e-4 | -3.12 | 6.42 | 1.17e-2 | -1.93 |
| 2 | 1.000 | 31.94 | 12.65 | 1.56e-4 | -3.81 | 8.94 | 2.03e-3 | -2.69 | |
| 3 | 1.000 | 29.66 | 10.69 | 6.05e-4 | -3.22 | 6.96 | 8.04e-3 | -2.09 | |
NGS expression data
Expression of miR160c transcript in NGS dataSequences
miRNA transcript
GUUAUGCCUGGCUCCCUGUAUGCCACGAGUGGAUACCGAUUUUGGUUUUAAAAUCGGCUGCCGGUGGCGUACAAGGAGUCAAGCAUGACCAGAAGCAUAUGGUAACUCAAUUGCAUCUACAACUAAAUAUGAAAUAUCUAGUUAUGUAUAUGGUUUGUGUUCUAUAAGAUGUUUUCUUCGAAUUUUGAUUUUUGUGUUCCAUUUGAAUAAGCAAACAGAAAUAAAACCAAGUUUUGUAGUUUUUUGCUUUCUUUCUGAUUUGGUC
Short sequences
| sequence | type | arm | start | end |
|---|---|---|---|---|
UGCCUGGCUCCCUGUAUGCCA | miRNA | 5 | 25 | |
TGGCGTACAAGGAGTCAAGCA | primer | 5p | 65 | 85 |
ACCAAATCAGAAAGAAAGCAAAAAAC | primer | 3p | 239 | 264 |
For experimental procedure see:Materials and Methods
Cross-references
| miRBase | miRBase record |
| ASRP | ASRP record |
| PMRD | PMRD record |
| TAIR | TAIR record |
| PubMed Entrez | The most up-to-date article(s) about miR160c found in PubMed |